Skin and lung fibrosis induced by bleomycin in mice: a systematic review
Objective. Scleroderma, or systemic sclerosis (SSc), is a chronic autoimmune connective disease with an unknown etiology and poorly understood pathogenesis. The striking array of autoimmune, vascular, and fibrotic changes that develop in almost all patients makes SSc unique among connective tissue...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2024-03-01
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| Series: | Reumatismo |
| Subjects: | |
| Online Access: | https://www.reumatismo.org/reuma/article/view/1642 |
| _version_ | 1797248671434145792 |
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| author | S. Gülle A. Çelik M. Birlik O. Yılmaz |
| author_facet | S. Gülle A. Çelik M. Birlik O. Yılmaz |
| author_sort | S. Gülle |
| collection | DOAJ |
| description |
Objective. Scleroderma, or systemic sclerosis (SSc), is a chronic autoimmune connective disease with an unknown etiology and poorly understood pathogenesis. The striking array of autoimmune, vascular, and fibrotic changes that develop in almost all patients makes SSc unique among connective tissue diseases. Although no animal model developed for SSc to date fully represents all features of human disease, some animal models that demonstrate features of SSc may help to better understand the pathogenesis of the disease and to develop new therapeutic options. In this review, we aimed to evaluate skin fibrosis and lung involvement in a bleomycin (BLM)-induced mouse model and to evaluate the differences between studies.
Methods. A systematic literature review (PRISMA guideline) on PubMed and EMBASE (until May 2023, without limits) was performed. A primary literature search was conducted using the PubMed and EMBASE databases for all articles published from 1990 to May 2023. Review articles, human studies, and non-dermatological studies were excluded. Of the 38 non-duplicated studies, 20 articles were included.
Results. Among inducible animal models, the BLM-induced SSc is still the most widely used. In recent years, the measurement of tissue thickness between the epidermal-dermal junction and the dermal-adipose tissue junction (dermal layer) has become more widely accepted.
Conclusions. In animal studies, it is important to simultaneously evaluate lung tissues in addition to skin fibrosis induced in mice by subcutaneous BLM application, following the 3R (replacement, reduction, and refinement) principle to avoid cruelty to animals.
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| first_indexed | 2024-04-24T20:18:18Z |
| format | Article |
| id | doaj.art-3f03d72e557c4107acfffdacb90f2aae |
| institution | Directory Open Access Journal |
| issn | 0048-7449 2240-2683 |
| language | English |
| last_indexed | 2024-04-24T20:18:18Z |
| publishDate | 2024-03-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Reumatismo |
| spelling | doaj.art-3f03d72e557c4107acfffdacb90f2aae2024-03-22T13:03:50ZengPAGEPress PublicationsReumatismo0048-74492240-26832024-03-0176110.4081/reumatismo.2024.1642Skin and lung fibrosis induced by bleomycin in mice: a systematic reviewS. Gülle0A. Çelik1M. Birlik2O. Yılmaz3Division of Rheumatology, Department of Internal Medicine, Dokuz Eylul University School of Medicine, Izmir; Department of Laboratory Animal Science, Dokuz Eylul University School of Medicine, IzmirDepartment of Laboratory Animal Science, Dokuz Eylul University School of Medicine, IzmirDivision of Rheumatology, Department of Internal Medicine, Dokuz Eylul University School of Medicine, IzmirDepartment of Laboratory Animal Science, Dokuz Eylul University School of Medicine, Izmir Objective. Scleroderma, or systemic sclerosis (SSc), is a chronic autoimmune connective disease with an unknown etiology and poorly understood pathogenesis. The striking array of autoimmune, vascular, and fibrotic changes that develop in almost all patients makes SSc unique among connective tissue diseases. Although no animal model developed for SSc to date fully represents all features of human disease, some animal models that demonstrate features of SSc may help to better understand the pathogenesis of the disease and to develop new therapeutic options. In this review, we aimed to evaluate skin fibrosis and lung involvement in a bleomycin (BLM)-induced mouse model and to evaluate the differences between studies. Methods. A systematic literature review (PRISMA guideline) on PubMed and EMBASE (until May 2023, without limits) was performed. A primary literature search was conducted using the PubMed and EMBASE databases for all articles published from 1990 to May 2023. Review articles, human studies, and non-dermatological studies were excluded. Of the 38 non-duplicated studies, 20 articles were included. Results. Among inducible animal models, the BLM-induced SSc is still the most widely used. In recent years, the measurement of tissue thickness between the epidermal-dermal junction and the dermal-adipose tissue junction (dermal layer) has become more widely accepted. Conclusions. In animal studies, it is important to simultaneously evaluate lung tissues in addition to skin fibrosis induced in mice by subcutaneous BLM application, following the 3R (replacement, reduction, and refinement) principle to avoid cruelty to animals. https://www.reumatismo.org/reuma/article/view/1642Sclerodermamurinebleomycinskin thicknesspulmonary fibrosisanimal model |
| spellingShingle | S. Gülle A. Çelik M. Birlik O. Yılmaz Skin and lung fibrosis induced by bleomycin in mice: a systematic review Reumatismo Scleroderma murine bleomycin skin thickness pulmonary fibrosis animal model |
| title | Skin and lung fibrosis induced by bleomycin in mice: a systematic review |
| title_full | Skin and lung fibrosis induced by bleomycin in mice: a systematic review |
| title_fullStr | Skin and lung fibrosis induced by bleomycin in mice: a systematic review |
| title_full_unstemmed | Skin and lung fibrosis induced by bleomycin in mice: a systematic review |
| title_short | Skin and lung fibrosis induced by bleomycin in mice: a systematic review |
| title_sort | skin and lung fibrosis induced by bleomycin in mice a systematic review |
| topic | Scleroderma murine bleomycin skin thickness pulmonary fibrosis animal model |
| url | https://www.reumatismo.org/reuma/article/view/1642 |
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