Staphylococcus aureus produces membrane-derived vesicles that induce host cell death.
Gram-negative bacteria produce outer membrane vesicles that play a role in the delivery of virulence factors to host cells. However, little is known about the membrane-derived vesicles (MVs) produced by gram-positive bacteria. The present study examined the production of MVs from Staphylococcus aure...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3218073?pdf=render |
| _version_ | 1818160462345273344 |
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| author | Mamata Gurung Dong Chan Moon Chi Won Choi Jung Hwa Lee Yong Chul Bae Jungmin Kim Yoo Chul Lee Sung Yong Seol Dong Taek Cho Seung Il Kim Je Chul Lee |
| author_facet | Mamata Gurung Dong Chan Moon Chi Won Choi Jung Hwa Lee Yong Chul Bae Jungmin Kim Yoo Chul Lee Sung Yong Seol Dong Taek Cho Seung Il Kim Je Chul Lee |
| author_sort | Mamata Gurung |
| collection | DOAJ |
| description | Gram-negative bacteria produce outer membrane vesicles that play a role in the delivery of virulence factors to host cells. However, little is known about the membrane-derived vesicles (MVs) produced by gram-positive bacteria. The present study examined the production of MVs from Staphylococcus aureus and investigated the delivery of MVs to host cells and subsequent cytotoxicity. Four S. aureus strains tested, two type strains and two clinical isolates, produced spherical nanovesicles during in vitro culture. MVs were also produced during in vivo infection of a clinical S. aureus isolate in a mouse pneumonia model. Proteomic analysis showed that 143 different proteins were identified in the S. aureus-derived MVs. S. aureus MVs were interacted with the plasma membrane of host cells via a cholesterol-rich membrane microdomain and then delivered their component protein A to host cells within 30 min. Intact S. aureus MVs induced apoptosis of HEp-2 cells in a dose-dependent manner, whereas lysed MVs neither delivered their component into the cytosol of host cells nor induced cytotoxicity. In conclusion, this study is the first report that S. aureus MVs are an important vehicle for delivery of bacterial effector molecules to host cells. |
| first_indexed | 2024-12-11T16:02:16Z |
| format | Article |
| id | doaj.art-3f0551a37a9a4eb6b45c5e9250dc3717 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T16:02:16Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3f0551a37a9a4eb6b45c5e9250dc37172022-12-22T00:59:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01611e2795810.1371/journal.pone.0027958Staphylococcus aureus produces membrane-derived vesicles that induce host cell death.Mamata GurungDong Chan MoonChi Won ChoiJung Hwa LeeYong Chul BaeJungmin KimYoo Chul LeeSung Yong SeolDong Taek ChoSeung Il KimJe Chul LeeGram-negative bacteria produce outer membrane vesicles that play a role in the delivery of virulence factors to host cells. However, little is known about the membrane-derived vesicles (MVs) produced by gram-positive bacteria. The present study examined the production of MVs from Staphylococcus aureus and investigated the delivery of MVs to host cells and subsequent cytotoxicity. Four S. aureus strains tested, two type strains and two clinical isolates, produced spherical nanovesicles during in vitro culture. MVs were also produced during in vivo infection of a clinical S. aureus isolate in a mouse pneumonia model. Proteomic analysis showed that 143 different proteins were identified in the S. aureus-derived MVs. S. aureus MVs were interacted with the plasma membrane of host cells via a cholesterol-rich membrane microdomain and then delivered their component protein A to host cells within 30 min. Intact S. aureus MVs induced apoptosis of HEp-2 cells in a dose-dependent manner, whereas lysed MVs neither delivered their component into the cytosol of host cells nor induced cytotoxicity. In conclusion, this study is the first report that S. aureus MVs are an important vehicle for delivery of bacterial effector molecules to host cells.http://europepmc.org/articles/PMC3218073?pdf=render |
| spellingShingle | Mamata Gurung Dong Chan Moon Chi Won Choi Jung Hwa Lee Yong Chul Bae Jungmin Kim Yoo Chul Lee Sung Yong Seol Dong Taek Cho Seung Il Kim Je Chul Lee Staphylococcus aureus produces membrane-derived vesicles that induce host cell death. PLoS ONE |
| title | Staphylococcus aureus produces membrane-derived vesicles that induce host cell death. |
| title_full | Staphylococcus aureus produces membrane-derived vesicles that induce host cell death. |
| title_fullStr | Staphylococcus aureus produces membrane-derived vesicles that induce host cell death. |
| title_full_unstemmed | Staphylococcus aureus produces membrane-derived vesicles that induce host cell death. |
| title_short | Staphylococcus aureus produces membrane-derived vesicles that induce host cell death. |
| title_sort | staphylococcus aureus produces membrane derived vesicles that induce host cell death |
| url | http://europepmc.org/articles/PMC3218073?pdf=render |
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