Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its Application
Levofloxacin (LVX) and amphotericin B (AMB) have been widely used to treat bacterial and fungal infections in the clinic. Herein, we report, for the first time, chitosan films loaded with AMB and LVX as wound dressings to combat antimicrobial infections. Additionally, we developed and validated a hi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/11/2497 |
| _version_ | 1827643877075451904 |
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| author | Ke Peng Mingshan Li Achmad Himawan Juan Domínguez-Robles Lalitkumar K. Vora Ross Duncan Xianbing Dai Chunyang Zhang Li Zhao Luchi Li Eneko Larrañeta Ryan F. Donnelly |
| author_facet | Ke Peng Mingshan Li Achmad Himawan Juan Domínguez-Robles Lalitkumar K. Vora Ross Duncan Xianbing Dai Chunyang Zhang Li Zhao Luchi Li Eneko Larrañeta Ryan F. Donnelly |
| author_sort | Ke Peng |
| collection | DOAJ |
| description | Levofloxacin (LVX) and amphotericin B (AMB) have been widely used to treat bacterial and fungal infections in the clinic. Herein, we report, for the first time, chitosan films loaded with AMB and LVX as wound dressings to combat antimicrobial infections. Additionally, we developed and validated a high-performance liquid chromatography (HPLC) method coupled with a UV detector to simultaneously quantify both AMB and LVX. The method is easy, precise, accurate and linear for both drugs at a concentration range of 0.7–5 µg/mL. The validated method was used to analyse the drug release, ex vivo deposition and permeation from the chitosan films. LVX was released completely from the chitosan film after a week, while approximately 60% of the AMB was released. Ex vivo deposition study revealed that, after 24-hour application, 20.96 ± 13.54 µg of LVX and approximately 0.35 ± 0.04 µg of AMB was deposited in porcine skin. Approximately 0.58 ± 0.16 µg of LVX permeated through the skin. AMB was undetectable in the receptor compartment due to its poor solubility and permeability. Furthermore, chitosan films loaded with AMB and LVX were found to be able to inhibit the growth of both <i>Candida albicans</i> and <i>Staphylococcus aureus</i>, indicating their potential for antimicrobial applications. |
| first_indexed | 2024-03-09T18:03:50Z |
| format | Article |
| id | doaj.art-3f08c74fa70b45bd88796d9dbec3b2c6 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T18:03:50Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-3f08c74fa70b45bd88796d9dbec3b2c62023-11-24T09:36:43ZengMDPI AGPharmaceutics1999-49232022-11-011411249710.3390/pharmaceutics14112497Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its ApplicationKe Peng0Mingshan Li1Achmad Himawan2Juan Domínguez-Robles3Lalitkumar K. Vora4Ross Duncan5Xianbing Dai6Chunyang Zhang7Li Zhao8Luchi Li9Eneko Larrañeta10Ryan F. Donnelly11School of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKSchool of Pharmacy, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UKLevofloxacin (LVX) and amphotericin B (AMB) have been widely used to treat bacterial and fungal infections in the clinic. Herein, we report, for the first time, chitosan films loaded with AMB and LVX as wound dressings to combat antimicrobial infections. Additionally, we developed and validated a high-performance liquid chromatography (HPLC) method coupled with a UV detector to simultaneously quantify both AMB and LVX. The method is easy, precise, accurate and linear for both drugs at a concentration range of 0.7–5 µg/mL. The validated method was used to analyse the drug release, ex vivo deposition and permeation from the chitosan films. LVX was released completely from the chitosan film after a week, while approximately 60% of the AMB was released. Ex vivo deposition study revealed that, after 24-hour application, 20.96 ± 13.54 µg of LVX and approximately 0.35 ± 0.04 µg of AMB was deposited in porcine skin. Approximately 0.58 ± 0.16 µg of LVX permeated through the skin. AMB was undetectable in the receptor compartment due to its poor solubility and permeability. Furthermore, chitosan films loaded with AMB and LVX were found to be able to inhibit the growth of both <i>Candida albicans</i> and <i>Staphylococcus aureus</i>, indicating their potential for antimicrobial applications.https://www.mdpi.com/1999-4923/14/11/2497amphotericin Blevofloxacinchitosan filmwound dressingantimicrobial |
| spellingShingle | Ke Peng Mingshan Li Achmad Himawan Juan Domínguez-Robles Lalitkumar K. Vora Ross Duncan Xianbing Dai Chunyang Zhang Li Zhao Luchi Li Eneko Larrañeta Ryan F. Donnelly Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its Application Pharmaceutics amphotericin B levofloxacin chitosan film wound dressing antimicrobial |
| title | Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its Application |
| title_full | Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its Application |
| title_fullStr | Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its Application |
| title_full_unstemmed | Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its Application |
| title_short | Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its Application |
| title_sort | amphotericin b and levofloxacin loaded chitosan films for potential use in antimicrobial wound dressings analytical method development and its application |
| topic | amphotericin B levofloxacin chitosan film wound dressing antimicrobial |
| url | https://www.mdpi.com/1999-4923/14/11/2497 |
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