Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis
Patients with rheumatoid arthritis (RA) show autoantibodies against post-translational protein modifications (PTMs), such as anti-citrullinated protein antibodies. However, the range of recognized PTMs is unknown. Here, we addressed four PTMs: chlorination, non-enzymatic glycation, nitration, and ho...
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MDPI AG
2022-01-01
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| author | Lorena Rodríguez-Martínez Cristina Regueiro Sámer Amhaz-Escanlar Carmen Pena Paloma Herbello-Hermelo Antonio Moreda-Piñeiro Javier Rodriguez-Garcia Antonio Mera-Varela Eva Pérez-Pampín Antonio González |
| author_facet | Lorena Rodríguez-Martínez Cristina Regueiro Sámer Amhaz-Escanlar Carmen Pena Paloma Herbello-Hermelo Antonio Moreda-Piñeiro Javier Rodriguez-Garcia Antonio Mera-Varela Eva Pérez-Pampín Antonio González |
| author_sort | Lorena Rodríguez-Martínez |
| collection | DOAJ |
| description | Patients with rheumatoid arthritis (RA) show autoantibodies against post-translational protein modifications (PTMs), such as anti-citrullinated protein antibodies. However, the range of recognized PTMs is unknown. Here, we addressed four PTMs: chlorination, non-enzymatic glycation, nitration, and homocysteinylation, identified as targets of atypical RA autoantibodies in studies whose protocols we have followed. The modified antigens included collagen type II, an extract of synovial proteins and a selection of peptides. We interpreted the results according to the optical density (OD) obtained in an enzyme-linked immunosorbent assay ( ELISA) with the modified antigen and the corrected OD obtained after subtracting the reactivity against the unmodified antigen. The results showed evidence of specific antibodies against glycated collagen type II, as the corrected ODs were higher in the 182 patients with RA than in the 164 healthy controls (<i>p</i> = 0.0003). However, the relevance of these antibodies was doubtful because the magnitude of the specific signal was small (median OD = 0.072 vs. 0.027, respectively). There were no specific antibodies against any of the other three PTMs. Therefore, our results showed that the four PTMs are not inducing a significant autoantibody response in patients with RA. These results indicated that the repertoire of PTM autoantigens in RA is restricted. |
| first_indexed | 2024-03-09T22:11:12Z |
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| last_indexed | 2024-03-09T22:11:12Z |
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| series | Diagnostics |
| spelling | doaj.art-3f09cc9b925e4015b598fe39b05e58482023-11-23T19:30:38ZengMDPI AGDiagnostics2075-44182022-01-0112235210.3390/diagnostics12020352Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid ArthritisLorena Rodríguez-Martínez0Cristina Regueiro1Sámer Amhaz-Escanlar2Carmen Pena3Paloma Herbello-Hermelo4Antonio Moreda-Piñeiro5Javier Rodriguez-Garcia6Antonio Mera-Varela7Eva Pérez-Pampín8Antonio González9Experimental and Observational Rheumatology and Rheumatology Unit, Instituto de Investigacion Sanitaria-Hospital Clínico Universitario de Santiago (IDIS), 15706 Santiago de Compostela, SpainExperimental and Observational Rheumatology and Rheumatology Unit, Instituto de Investigacion Sanitaria-Hospital Clínico Universitario de Santiago (IDIS), 15706 Santiago de Compostela, SpainDepartment of Orthopedic Surgery and Traumatology, Instituto de Investigación Sanitaria-Hospital Clínico Universitario de Santiago (IDIS), 15706 Santiago de Compostela, SpainExperimental and Observational Rheumatology and Rheumatology Unit, Instituto de Investigacion Sanitaria-Hospital Clínico Universitario de Santiago (IDIS), 15706 Santiago de Compostela, SpainTrace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainTrace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainDepartment of Clinical Analysis, Instituto de Investigación Sanitaria-Hospital Clínico Universitario de Santiago (IDIS), 15706 Santiago de Compostela, SpainExperimental and Observational Rheumatology and Rheumatology Unit, Instituto de Investigacion Sanitaria-Hospital Clínico Universitario de Santiago (IDIS), 15706 Santiago de Compostela, SpainExperimental and Observational Rheumatology and Rheumatology Unit, Instituto de Investigacion Sanitaria-Hospital Clínico Universitario de Santiago (IDIS), 15706 Santiago de Compostela, SpainExperimental and Observational Rheumatology and Rheumatology Unit, Instituto de Investigacion Sanitaria-Hospital Clínico Universitario de Santiago (IDIS), 15706 Santiago de Compostela, SpainPatients with rheumatoid arthritis (RA) show autoantibodies against post-translational protein modifications (PTMs), such as anti-citrullinated protein antibodies. However, the range of recognized PTMs is unknown. Here, we addressed four PTMs: chlorination, non-enzymatic glycation, nitration, and homocysteinylation, identified as targets of atypical RA autoantibodies in studies whose protocols we have followed. The modified antigens included collagen type II, an extract of synovial proteins and a selection of peptides. We interpreted the results according to the optical density (OD) obtained in an enzyme-linked immunosorbent assay ( ELISA) with the modified antigen and the corrected OD obtained after subtracting the reactivity against the unmodified antigen. The results showed evidence of specific antibodies against glycated collagen type II, as the corrected ODs were higher in the 182 patients with RA than in the 164 healthy controls (<i>p</i> = 0.0003). However, the relevance of these antibodies was doubtful because the magnitude of the specific signal was small (median OD = 0.072 vs. 0.027, respectively). There were no specific antibodies against any of the other three PTMs. Therefore, our results showed that the four PTMs are not inducing a significant autoantibody response in patients with RA. These results indicated that the repertoire of PTM autoantigens in RA is restricted.https://www.mdpi.com/2075-4418/12/2/352rheumatoid arthritisautoantibodiespost-translational protein modificationschlorinationnon-enzymatic glycationnitration |
| spellingShingle | Lorena Rodríguez-Martínez Cristina Regueiro Sámer Amhaz-Escanlar Carmen Pena Paloma Herbello-Hermelo Antonio Moreda-Piñeiro Javier Rodriguez-Garcia Antonio Mera-Varela Eva Pérez-Pampín Antonio González Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis Diagnostics rheumatoid arthritis autoantibodies post-translational protein modifications chlorination non-enzymatic glycation nitration |
| title | Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis |
| title_full | Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis |
| title_fullStr | Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis |
| title_full_unstemmed | Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis |
| title_short | Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis |
| title_sort | antibodies against 4 atypical post translational protein modifications in patients with rheumatoid arthritis |
| topic | rheumatoid arthritis autoantibodies post-translational protein modifications chlorination non-enzymatic glycation nitration |
| url | https://www.mdpi.com/2075-4418/12/2/352 |
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