Biosynthesis and Optimization of PolyHydroxyalkanoate (PHA) Production by Acinetobacter lwoffii Isolate
Polyhydroxyalkanoate (PHA) is an eco-friendly polymer that has various important biomedical uses, as well as biodegradability, drug delivery, and medical implants. It is a kind of polyester synthesized by various microorganisms as energy reserve material under inappropriate conditions. This study fo...
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| Format: | Article |
| Language: | English |
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University of Technology, Baghdad
2023-03-01
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| Series: | Journal of Applied Sciences and Nanotechnology |
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| Online Access: | https://jasn.uotechnology.edu.iq/article_22932_5cfb2bc20b390a61d01867d469e5c1b9.pdf |
| _version_ | 1797871567930130432 |
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| author | Karrar Hameed Iman Gatea Amal Hussein |
| author_facet | Karrar Hameed Iman Gatea Amal Hussein |
| author_sort | Karrar Hameed |
| collection | DOAJ |
| description | Polyhydroxyalkanoate (PHA) is an eco-friendly polymer that has various important biomedical uses, as well as biodegradability, drug delivery, and medical implants. It is a kind of polyester synthesized by various microorganisms as energy reserve material under inappropriate conditions. This study focused on testing wastecooking oilas the source of carbon to optimize PHA production. The tests included the most important environmental components within the culture medium affecting the growth of the bacterial isolate under the experiment and its ability to produce a polymer. Results revealed that the cultivation of <em>Acinetobacter lwoffii</em>, pre-isolated from hydrocarbon contaminated soil, under optimum conditions showed the highest productivity after 72 hours. Corn oil waste as the carbon and urea as the source of nitrogen were discovered to be the best nutritive sources for concentrated PHA production<em>, </em>with, 2% and 0.5 g/L as the best carbon and nitrogen sources concentrations, respectively. Through the results, it was found that there is an important role for the change, both qualitatively and quantitatively, in the components of the nutrient medium and the surrounding condition in increasing the efficiency of isolation, as it had a significant role in increasing the efficiency of the isolate. There was a significant increase in the PHA content ranging from 10 to 75% and the production of biomass to 3.6 g/L. respectively. Finally, this study concluded that the use of bacterial isolate in the production of PHA can contribute to solving the critical problem of environmental pollution caused by the use of industrial plastic and replacing it with environmentally friendly and low-cost materials. |
| first_indexed | 2024-04-10T00:44:51Z |
| format | Article |
| id | doaj.art-3f0ac00bd92a42e8aff59b4c1c0aeeb0 |
| institution | Directory Open Access Journal |
| issn | 2788-6867 |
| language | English |
| last_indexed | 2024-04-10T00:44:51Z |
| publishDate | 2023-03-01 |
| publisher | University of Technology, Baghdad |
| record_format | Article |
| series | Journal of Applied Sciences and Nanotechnology |
| spelling | doaj.art-3f0ac00bd92a42e8aff59b4c1c0aeeb02023-03-13T22:31:29ZengUniversity of Technology, BaghdadJournal of Applied Sciences and Nanotechnology2788-68672023-03-01319010310.53293/jasn.2022.4970.116722932Biosynthesis and Optimization of PolyHydroxyalkanoate (PHA) Production by Acinetobacter lwoffii IsolateKarrar Hameed0Iman Gatea1Amal Hussein2Department of Applied Sciences, University of Technology – IraqEnvironment and Water Directorate, Ministry of Science and Technology – IraqDepartment of Applied Sciences, University of Technology – IraqPolyhydroxyalkanoate (PHA) is an eco-friendly polymer that has various important biomedical uses, as well as biodegradability, drug delivery, and medical implants. It is a kind of polyester synthesized by various microorganisms as energy reserve material under inappropriate conditions. This study focused on testing wastecooking oilas the source of carbon to optimize PHA production. The tests included the most important environmental components within the culture medium affecting the growth of the bacterial isolate under the experiment and its ability to produce a polymer. Results revealed that the cultivation of <em>Acinetobacter lwoffii</em>, pre-isolated from hydrocarbon contaminated soil, under optimum conditions showed the highest productivity after 72 hours. Corn oil waste as the carbon and urea as the source of nitrogen were discovered to be the best nutritive sources for concentrated PHA production<em>, </em>with, 2% and 0.5 g/L as the best carbon and nitrogen sources concentrations, respectively. Through the results, it was found that there is an important role for the change, both qualitatively and quantitatively, in the components of the nutrient medium and the surrounding condition in increasing the efficiency of isolation, as it had a significant role in increasing the efficiency of the isolate. There was a significant increase in the PHA content ranging from 10 to 75% and the production of biomass to 3.6 g/L. respectively. Finally, this study concluded that the use of bacterial isolate in the production of PHA can contribute to solving the critical problem of environmental pollution caused by the use of industrial plastic and replacing it with environmentally friendly and low-cost materials.https://jasn.uotechnology.edu.iq/article_22932_5cfb2bc20b390a61d01867d469e5c1b9.pdfacinetobacteroptimizationbioplasticoil wastepolyhydroxyalkanoate |
| spellingShingle | Karrar Hameed Iman Gatea Amal Hussein Biosynthesis and Optimization of PolyHydroxyalkanoate (PHA) Production by Acinetobacter lwoffii Isolate Journal of Applied Sciences and Nanotechnology acinetobacter optimization bioplastic oil waste polyhydroxyalkanoate |
| title | Biosynthesis and Optimization of PolyHydroxyalkanoate (PHA) Production by Acinetobacter lwoffii Isolate |
| title_full | Biosynthesis and Optimization of PolyHydroxyalkanoate (PHA) Production by Acinetobacter lwoffii Isolate |
| title_fullStr | Biosynthesis and Optimization of PolyHydroxyalkanoate (PHA) Production by Acinetobacter lwoffii Isolate |
| title_full_unstemmed | Biosynthesis and Optimization of PolyHydroxyalkanoate (PHA) Production by Acinetobacter lwoffii Isolate |
| title_short | Biosynthesis and Optimization of PolyHydroxyalkanoate (PHA) Production by Acinetobacter lwoffii Isolate |
| title_sort | biosynthesis and optimization of polyhydroxyalkanoate pha production by acinetobacter lwoffii isolate |
| topic | acinetobacter optimization bioplastic oil waste polyhydroxyalkanoate |
| url | https://jasn.uotechnology.edu.iq/article_22932_5cfb2bc20b390a61d01867d469e5c1b9.pdf |
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