Comparative Study of the Convertibility of Agricultural Residues and Other Cellulose-Containing Materials in Hydrolysis with <i>Penicillium verruculosum</i> Cellulase Complex
Non-edible cellulose-containing biomass is a promising and abundant feedstock for simple sugar production. This study presents the results of different cellulose-containing materials (CCM) hydrolysis experiments with <i>P. verruculosum</i> enzyme complexes in laboratory conditions. Among...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/11/1712 |
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| author | Dmitrii O. Osipov Gleb S. Dotsenko Olga A. Sinitsyna Elena G. Kondratieva Ivan N. Zorov Igor A. Shashkov Aidar D. Satrutdinov Arkady P. Sinitsyn |
| author_facet | Dmitrii O. Osipov Gleb S. Dotsenko Olga A. Sinitsyna Elena G. Kondratieva Ivan N. Zorov Igor A. Shashkov Aidar D. Satrutdinov Arkady P. Sinitsyn |
| author_sort | Dmitrii O. Osipov |
| collection | DOAJ |
| description | Non-edible cellulose-containing biomass is a promising and abundant feedstock for simple sugar production. This study presents the results of different cellulose-containing materials (CCM) hydrolysis experiments with <i>P. verruculosum</i> enzyme complexes in laboratory conditions. Among the non-pretreated substrates, only a few had a relatively high convertibility—soy bean husks (31%) and sugar beat pulp (20%)—while wheat straw, oat husks, sunflower peals, and corn stalks had a low convertibility of 3% to 12%. This indicates that a major part of CCM needs pretreatment. Steam-exploded (with Ca(OH)<sub>2</sub>) soy bean and oat husks (76% and 58%), fine ball-milled aspen wood and nitric acid-pretreated aspen wood (62% and 78%), and steam-exploded (with sulfuric acid) corn stalks (55%) had a high convertibility. Woody biomass pretreated with pulp and paper mills also had a high convertibility (56–78%)—e.g., never dried kraft hardwood and softwood pulp (both bleached and unbleached). These results demonstrate that effective cellulose-containing material processing into simple sugars is possible. Simple sugars derived from CCM using <i>P. verruculosum</i> preparation are a promising feedstock for the microbiological production of biofuels (bioethanol and biobutanol), aminoacids, and organic acids (e.g., lactic acid for polylactic acid production). |
| first_indexed | 2024-03-10T15:05:57Z |
| format | Article |
| id | doaj.art-3f106c775b344480a9992cbe3800c8c8 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T15:05:57Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-3f106c775b344480a9992cbe3800c8c82023-11-20T19:46:59ZengMDPI AGAgronomy2073-43952020-11-011011171210.3390/agronomy10111712Comparative Study of the Convertibility of Agricultural Residues and Other Cellulose-Containing Materials in Hydrolysis with <i>Penicillium verruculosum</i> Cellulase ComplexDmitrii O. Osipov0Gleb S. Dotsenko1Olga A. Sinitsyna2Elena G. Kondratieva3Ivan N. Zorov4Igor A. Shashkov5Aidar D. Satrutdinov6Arkady P. Sinitsyn7Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaDepartment of Chemistry, M. V. Lomonosov Moscow State University, 119991 Moscow, RussiaDepartment of Chemistry, M. V. Lomonosov Moscow State University, 119991 Moscow, RussiaFederal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, RussiaNon-edible cellulose-containing biomass is a promising and abundant feedstock for simple sugar production. This study presents the results of different cellulose-containing materials (CCM) hydrolysis experiments with <i>P. verruculosum</i> enzyme complexes in laboratory conditions. Among the non-pretreated substrates, only a few had a relatively high convertibility—soy bean husks (31%) and sugar beat pulp (20%)—while wheat straw, oat husks, sunflower peals, and corn stalks had a low convertibility of 3% to 12%. This indicates that a major part of CCM needs pretreatment. Steam-exploded (with Ca(OH)<sub>2</sub>) soy bean and oat husks (76% and 58%), fine ball-milled aspen wood and nitric acid-pretreated aspen wood (62% and 78%), and steam-exploded (with sulfuric acid) corn stalks (55%) had a high convertibility. Woody biomass pretreated with pulp and paper mills also had a high convertibility (56–78%)—e.g., never dried kraft hardwood and softwood pulp (both bleached and unbleached). These results demonstrate that effective cellulose-containing material processing into simple sugars is possible. Simple sugars derived from CCM using <i>P. verruculosum</i> preparation are a promising feedstock for the microbiological production of biofuels (bioethanol and biobutanol), aminoacids, and organic acids (e.g., lactic acid for polylactic acid production).https://www.mdpi.com/2073-4395/10/11/1712cellulose-containing materials<i>Penicillium verruculosum</i>recombinant enzymespretreatment |
| spellingShingle | Dmitrii O. Osipov Gleb S. Dotsenko Olga A. Sinitsyna Elena G. Kondratieva Ivan N. Zorov Igor A. Shashkov Aidar D. Satrutdinov Arkady P. Sinitsyn Comparative Study of the Convertibility of Agricultural Residues and Other Cellulose-Containing Materials in Hydrolysis with <i>Penicillium verruculosum</i> Cellulase Complex Agronomy cellulose-containing materials <i>Penicillium verruculosum</i> recombinant enzymes pretreatment |
| title | Comparative Study of the Convertibility of Agricultural Residues and Other Cellulose-Containing Materials in Hydrolysis with <i>Penicillium verruculosum</i> Cellulase Complex |
| title_full | Comparative Study of the Convertibility of Agricultural Residues and Other Cellulose-Containing Materials in Hydrolysis with <i>Penicillium verruculosum</i> Cellulase Complex |
| title_fullStr | Comparative Study of the Convertibility of Agricultural Residues and Other Cellulose-Containing Materials in Hydrolysis with <i>Penicillium verruculosum</i> Cellulase Complex |
| title_full_unstemmed | Comparative Study of the Convertibility of Agricultural Residues and Other Cellulose-Containing Materials in Hydrolysis with <i>Penicillium verruculosum</i> Cellulase Complex |
| title_short | Comparative Study of the Convertibility of Agricultural Residues and Other Cellulose-Containing Materials in Hydrolysis with <i>Penicillium verruculosum</i> Cellulase Complex |
| title_sort | comparative study of the convertibility of agricultural residues and other cellulose containing materials in hydrolysis with i penicillium verruculosum i cellulase complex |
| topic | cellulose-containing materials <i>Penicillium verruculosum</i> recombinant enzymes pretreatment |
| url | https://www.mdpi.com/2073-4395/10/11/1712 |
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