PWRN1 Suppressed Cancer Cell Proliferation and Migration in Glioblastoma by Inversely Regulating hsa-miR-21-5p
Jianxin Jiang,* Xiaolin Wang,* Jun Lu Department of Neurosurgery, Taizhou People’s Hospital, Taizhou, Jiangsu Province 225300, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jun LuDepartment of Neurosurgery, Taizhou People’s Hos...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-07-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/pwrn1-suppressed-cancer-cell-proliferation-and-migration-in-glioblasto-peer-reviewed-article-CMAR |
| _version_ | 1811220264413822976 |
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| author | Jiang J Wang X Lu J |
| author_facet | Jiang J Wang X Lu J |
| author_sort | Jiang J |
| collection | DOAJ |
| description | Jianxin Jiang,* Xiaolin Wang,* Jun Lu Department of Neurosurgery, Taizhou People’s Hospital, Taizhou, Jiangsu Province 225300, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jun LuDepartment of Neurosurgery, Taizhou People’s Hospital, M.D 366 Taihu Road, Taizhou, Jiangsu Province 225300, People’s Republic of ChinaTel +86-523-89890321Email lujun83@aol.comObjective: To evaluate the expression and function of long noncoding RNA (lncRNA) Prader-Willi region non-protein coding RNA 1 (PWRN1) in human glioblastoma (GBM).Materials and Methods: QRT-PCR was applied to assess PWRN1 expression in human GBM tumors and GBM cell lines. PWRN1 was overexpressed by lentiviral infection in LN-229 and U-251 cells to evaluate its effect on GBM cell proliferation and migration in vitro, and xenograft in vivo. The endogenously competing target of PWRN1, human microRNA-21-5p (hsa-miR-21-5p) was evaluated by dual-luciferase activity assay and qRT-PCR. Also, hsa-miR-21-5p was upregulated in PWRN1-overexpressed GBM cells to evaluate the functional involvement of hsa-miR-21-5p in PWRN1-mediated GBM cell proliferation and migration.Results: PWRN1 was downregulated in both human GBM tumors and GBM cell lines. In LN-229 and U-251, PWRN1 overexpression suppressed cancer cell proliferation and migration in vitro, and xenograft growth in vivo. Hsa-miR-21-5p was demonstrated to be the downstream competing target of PWRN1 in GBM. Conversely, upregulating hsa-miR-21-5p in LN-229 and U-251 cells reversed the tumor-suppressing effects of PWRN1 overexpression.Conclusion: PWRN1 is markedly downregulated in GBM. Overexpressing PWRN1 has tumor inhibitory effect on GBM cells, likely via reversely suppressing the expression of tumor oncogenic factor of hsa-miR-21-5p.Keywords: GBM, lncRNAs, PWRN1, microRNA, hsa-miR-21-5p, migration |
| first_indexed | 2024-04-12T07:39:58Z |
| format | Article |
| id | doaj.art-3f11e085d900452291b4dff136858e39 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-04-12T07:39:58Z |
| publishDate | 2020-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-3f11e085d900452291b4dff136858e392022-12-22T03:41:51ZengDove Medical PressCancer Management and Research1179-13222020-07-01Volume 125313532255005PWRN1 Suppressed Cancer Cell Proliferation and Migration in Glioblastoma by Inversely Regulating hsa-miR-21-5pJiang JWang XLu JJianxin Jiang,* Xiaolin Wang,* Jun Lu Department of Neurosurgery, Taizhou People’s Hospital, Taizhou, Jiangsu Province 225300, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jun LuDepartment of Neurosurgery, Taizhou People’s Hospital, M.D 366 Taihu Road, Taizhou, Jiangsu Province 225300, People’s Republic of ChinaTel +86-523-89890321Email lujun83@aol.comObjective: To evaluate the expression and function of long noncoding RNA (lncRNA) Prader-Willi region non-protein coding RNA 1 (PWRN1) in human glioblastoma (GBM).Materials and Methods: QRT-PCR was applied to assess PWRN1 expression in human GBM tumors and GBM cell lines. PWRN1 was overexpressed by lentiviral infection in LN-229 and U-251 cells to evaluate its effect on GBM cell proliferation and migration in vitro, and xenograft in vivo. The endogenously competing target of PWRN1, human microRNA-21-5p (hsa-miR-21-5p) was evaluated by dual-luciferase activity assay and qRT-PCR. Also, hsa-miR-21-5p was upregulated in PWRN1-overexpressed GBM cells to evaluate the functional involvement of hsa-miR-21-5p in PWRN1-mediated GBM cell proliferation and migration.Results: PWRN1 was downregulated in both human GBM tumors and GBM cell lines. In LN-229 and U-251, PWRN1 overexpression suppressed cancer cell proliferation and migration in vitro, and xenograft growth in vivo. Hsa-miR-21-5p was demonstrated to be the downstream competing target of PWRN1 in GBM. Conversely, upregulating hsa-miR-21-5p in LN-229 and U-251 cells reversed the tumor-suppressing effects of PWRN1 overexpression.Conclusion: PWRN1 is markedly downregulated in GBM. Overexpressing PWRN1 has tumor inhibitory effect on GBM cells, likely via reversely suppressing the expression of tumor oncogenic factor of hsa-miR-21-5p.Keywords: GBM, lncRNAs, PWRN1, microRNA, hsa-miR-21-5p, migrationhttps://www.dovepress.com/pwrn1-suppressed-cancer-cell-proliferation-and-migration-in-glioblasto-peer-reviewed-article-CMARgbmlncrnaspwrn1micrornahsa-mir-21-5pmigration |
| spellingShingle | Jiang J Wang X Lu J PWRN1 Suppressed Cancer Cell Proliferation and Migration in Glioblastoma by Inversely Regulating hsa-miR-21-5p Cancer Management and Research gbm lncrnas pwrn1 microrna hsa-mir-21-5p migration |
| title | PWRN1 Suppressed Cancer Cell Proliferation and Migration in Glioblastoma by Inversely Regulating hsa-miR-21-5p |
| title_full | PWRN1 Suppressed Cancer Cell Proliferation and Migration in Glioblastoma by Inversely Regulating hsa-miR-21-5p |
| title_fullStr | PWRN1 Suppressed Cancer Cell Proliferation and Migration in Glioblastoma by Inversely Regulating hsa-miR-21-5p |
| title_full_unstemmed | PWRN1 Suppressed Cancer Cell Proliferation and Migration in Glioblastoma by Inversely Regulating hsa-miR-21-5p |
| title_short | PWRN1 Suppressed Cancer Cell Proliferation and Migration in Glioblastoma by Inversely Regulating hsa-miR-21-5p |
| title_sort | pwrn1 suppressed cancer cell proliferation and migration in glioblastoma by inversely regulating hsa mir 21 5p |
| topic | gbm lncrnas pwrn1 microrna hsa-mir-21-5p migration |
| url | https://www.dovepress.com/pwrn1-suppressed-cancer-cell-proliferation-and-migration-in-glioblasto-peer-reviewed-article-CMAR |
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