Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification
For the on-site detection of aflatoxin B1 (AFB1), a DNA hydrogel was prepared as a biosensor substrate, while an AFB1 aptamer was used as the recognition element. An AFB1-responsive aptamer-cross-linked hydrogel sensor was constructed using an enzyme-linked signal amplification strategy; AFB1 binds...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157522001936 |
| _version_ | 1811264385512898560 |
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| author | Mengyao Zheng Hongmei Liu Jin Ye Baoxia Ni Yanli Xie Songxue Wang |
| author_facet | Mengyao Zheng Hongmei Liu Jin Ye Baoxia Ni Yanli Xie Songxue Wang |
| author_sort | Mengyao Zheng |
| collection | DOAJ |
| description | For the on-site detection of aflatoxin B1 (AFB1), a DNA hydrogel was prepared as a biosensor substrate, while an AFB1 aptamer was used as the recognition element. An AFB1-responsive aptamer-cross-linked hydrogel sensor was constructed using an enzyme-linked signal amplification strategy; AFB1 binds competitively to the aptamer, causing the hydrogel to undergo cleavage and release horseradish peroxidase (HRP). The addition of exonuclease I (ExoI) to the hydrogel causes the release of AFB1 from the aptamer, promoting additional hydrogel cleavage to release more HRP, ultimately catalysing the reaction between 3,3′,5,5′-tetramethylbenzidine and H2O2. The hydrogel sensor exhibited an outstanding sensitivity (limit of detection, 4.93 nM; dynamic range, 0–500 nM), and its selectivity towards seven other mycotoxins was confirmed. The feasibility and reliability were verified by measuring the AFB1 levels in peanut oil (recoveries, 89.59–95.66 %; relative standard deviation, <7%); the obtained results were comparable to those obtained by UPLC-HRMS. |
| first_indexed | 2024-04-12T20:02:07Z |
| format | Article |
| id | doaj.art-3f120f2fecb04263be133d39263176ad |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-12T20:02:07Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-3f120f2fecb04263be133d39263176ad2022-12-22T03:18:29ZengElsevierFood Chemistry: X2590-15752022-10-0115100395Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplificationMengyao Zheng0Hongmei Liu1Jin Ye2Baoxia Ni3Yanli Xie4Songxue Wang5College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China; Academy of National Food and Strategic Reserves Administration, Beijing 102600, ChinaAcademy of National Food and Strategic Reserves Administration, Beijing 102600, ChinaAcademy of National Food and Strategic Reserves Administration, Beijing 102600, China; Key Laboratory of Grain Information Processing and Control (Henan University of Technology), Ministry of Education, Zhengzhou 450001, China; Henan Key Laboratory of Grain Photoelectric Detection and Control, (Henan University of Technology), Zhengzhou 450001, ChinaAcademy of National Food and Strategic Reserves Administration, Beijing 102600, ChinaCollege of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China; Academy of National Food and Strategic Reserves Administration, Beijing 102600, China; Corresponding author at: College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China.For the on-site detection of aflatoxin B1 (AFB1), a DNA hydrogel was prepared as a biosensor substrate, while an AFB1 aptamer was used as the recognition element. An AFB1-responsive aptamer-cross-linked hydrogel sensor was constructed using an enzyme-linked signal amplification strategy; AFB1 binds competitively to the aptamer, causing the hydrogel to undergo cleavage and release horseradish peroxidase (HRP). The addition of exonuclease I (ExoI) to the hydrogel causes the release of AFB1 from the aptamer, promoting additional hydrogel cleavage to release more HRP, ultimately catalysing the reaction between 3,3′,5,5′-tetramethylbenzidine and H2O2. The hydrogel sensor exhibited an outstanding sensitivity (limit of detection, 4.93 nM; dynamic range, 0–500 nM), and its selectivity towards seven other mycotoxins was confirmed. The feasibility and reliability were verified by measuring the AFB1 levels in peanut oil (recoveries, 89.59–95.66 %; relative standard deviation, <7%); the obtained results were comparable to those obtained by UPLC-HRMS.http://www.sciencedirect.com/science/article/pii/S2590157522001936HydrogelEnzyme cascadeAptamerAFB1Visual detection |
| spellingShingle | Mengyao Zheng Hongmei Liu Jin Ye Baoxia Ni Yanli Xie Songxue Wang Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification Food Chemistry: X Hydrogel Enzyme cascade Aptamer AFB1 Visual detection |
| title | Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification |
| title_full | Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification |
| title_fullStr | Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification |
| title_full_unstemmed | Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification |
| title_short | Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification |
| title_sort | target responsive aptamer cross linked hydrogel sensors for the visual quantitative detection of aflatoxin b1 using exonuclease i triggered target cyclic amplification |
| topic | Hydrogel Enzyme cascade Aptamer AFB1 Visual detection |
| url | http://www.sciencedirect.com/science/article/pii/S2590157522001936 |
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