LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer
ObjectivesColorectal cancer(CRC) is a common malignant tumor. Recent studies have found that lncRNAs play an important role in the occurrence and development of colorectal cancer.MethodsBased on high-throughput sequencing results of fresh CRC tissues and adjacent tissues, we identified lncRNA-ENST00...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.960481/full |
| _version_ | 1811339480662016000 |
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| author | Weimin Wang Weimin Wang Zhen Zhou Zhen Zhou Xiaojun Dai Haibo Wang Jun Jin Ke Min Yunfan Wang Mengying Lv Yanqing Liu Yanqing Liu Yan Zhou Yan Zhou |
| author_facet | Weimin Wang Weimin Wang Zhen Zhou Zhen Zhou Xiaojun Dai Haibo Wang Jun Jin Ke Min Yunfan Wang Mengying Lv Yanqing Liu Yanqing Liu Yan Zhou Yan Zhou |
| author_sort | Weimin Wang |
| collection | DOAJ |
| description | ObjectivesColorectal cancer(CRC) is a common malignant tumor. Recent studies have found that lncRNAs play an important role in the occurrence and development of colorectal cancer.MethodsBased on high-throughput sequencing results of fresh CRC tissues and adjacent tissues, we identified lncRNA-ENST00000543604 (lncRNA 604) as the research object by qRT-PCR in CRC tissues and cells. We explored the mechanism of lncRNA 604 action by using luciferin reporter, qRT-PCR and Western blot assays. Kaplan-Meier survival analysis and a Cox regression model were used to analyze the correlation of lncRNA 604 and its regulatory molecules with the prognosis of and chemotherapy efficacy in CRC patients.ResultsIn this study, we found that the expression levels of lncRNA 604 were increased in CRC. LncRNA 604 could promote CRC cell proliferation and metastasis through the miRNA 564/AEG-1 or ZNF326/EMT signaling axis in vivo and in vitro. LncRNA 604 could predict the prognosis of CRC and was an independent negative factor. LncRNA 604 exerted a synergistic effect with miRNA 564 or ZNF326 on the prognosis of CRC. LncRNA 604 could improve chemoresistance by increasing the expression of AEG-1, NF-κB, and ERCC1.ConclusionsOur study demonstrated that lncRNA 604 could promote the progression of CRC via the lncRNA 604/miRNA 564/AEG-1/EMT or lncRNA 604/ZNF326/EMT signaling axis. LncRNA 604 could improve chemoresistance by increasing drug resistance protein expression. |
| first_indexed | 2024-04-13T18:27:19Z |
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| language | English |
| last_indexed | 2024-04-13T18:27:19Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-3f12be67fb1b412db2dfd10e112948112022-12-22T02:35:12ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-08-011210.3389/fonc.2022.960481960481LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancerWeimin Wang0Weimin Wang1Zhen Zhou2Zhen Zhou3Xiaojun Dai4Haibo Wang5Jun Jin6Ke Min7Yunfan Wang8Mengying Lv9Yanqing Liu10Yanqing Liu11Yan Zhou12Yan Zhou13Institute of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Jiangsu, ChinaDepartment of Oncology, Yixing Hospital Affiliated to Medical College of Yangzhou University, Yangzhou University, Jiangsu, ChinaInstitute of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Jiangsu, ChinaDepartment of Chinese Medicine, Nanjing University of Chinese Medicine, Jiangsu, Nanjing, ChinaInstitute of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Jiangsu, ChinaInstitute of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Jiangsu, ChinaDepartment of Oncology, Yixing Hospital Affiliated to Medical College of Yangzhou University, Yangzhou University, Jiangsu, ChinaDepartment of Oncology, Yixing Hospital Affiliated to Medical College of Yangzhou University, Yangzhou University, Jiangsu, ChinaDepartment of Oncology, Yixing Hospital Affiliated to Medical College of Yangzhou University, Yangzhou University, Jiangsu, ChinaInstitute of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Jiangsu, ChinaInstitute of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Jiangsu, ChinaDepartment of Oncology, Yixing Hospital Affiliated to Medical College of Yangzhou University, Yangzhou University, Jiangsu, ChinaInstitute of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Jiangsu, ChinaDepartment of Oncology, Yixing Hospital Affiliated to Medical College of Yangzhou University, Yangzhou University, Jiangsu, ChinaObjectivesColorectal cancer(CRC) is a common malignant tumor. Recent studies have found that lncRNAs play an important role in the occurrence and development of colorectal cancer.MethodsBased on high-throughput sequencing results of fresh CRC tissues and adjacent tissues, we identified lncRNA-ENST00000543604 (lncRNA 604) as the research object by qRT-PCR in CRC tissues and cells. We explored the mechanism of lncRNA 604 action by using luciferin reporter, qRT-PCR and Western blot assays. Kaplan-Meier survival analysis and a Cox regression model were used to analyze the correlation of lncRNA 604 and its regulatory molecules with the prognosis of and chemotherapy efficacy in CRC patients.ResultsIn this study, we found that the expression levels of lncRNA 604 were increased in CRC. LncRNA 604 could promote CRC cell proliferation and metastasis through the miRNA 564/AEG-1 or ZNF326/EMT signaling axis in vivo and in vitro. LncRNA 604 could predict the prognosis of CRC and was an independent negative factor. LncRNA 604 exerted a synergistic effect with miRNA 564 or ZNF326 on the prognosis of CRC. LncRNA 604 could improve chemoresistance by increasing the expression of AEG-1, NF-κB, and ERCC1.ConclusionsOur study demonstrated that lncRNA 604 could promote the progression of CRC via the lncRNA 604/miRNA 564/AEG-1/EMT or lncRNA 604/ZNF326/EMT signaling axis. LncRNA 604 could improve chemoresistance by increasing drug resistance protein expression.https://www.frontiersin.org/articles/10.3389/fonc.2022.960481/fullENST00000543604miRNA 564AEG-1ZNF 326colorectal cancerchemotherapy |
| spellingShingle | Weimin Wang Weimin Wang Zhen Zhou Zhen Zhou Xiaojun Dai Haibo Wang Jun Jin Ke Min Yunfan Wang Mengying Lv Yanqing Liu Yanqing Liu Yan Zhou Yan Zhou LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer Frontiers in Oncology ENST00000543604 miRNA 564 AEG-1 ZNF 326 colorectal cancer chemotherapy |
| title | LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer |
| title_full | LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer |
| title_fullStr | LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer |
| title_full_unstemmed | LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer |
| title_short | LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer |
| title_sort | lncrna enst00000543604 exerts a tumor promoting effect via mirna 564 aeg 1 or znf326 emt and predicts the prognosis of and chemotherapeutic effect in colorectal cancer |
| topic | ENST00000543604 miRNA 564 AEG-1 ZNF 326 colorectal cancer chemotherapy |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.960481/full |
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