Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis Plants
Soybean (<i>Glycine max</i>) is one of the important oil crops worldwide. In recent years, environmental stresses such as drought and soil salinization have severely deteriorated soybean yield and quality. We investigated the overexpression of the transcription factor GmTGA15 in response...
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MDPI AG
2021-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/1/170 |
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| author | Zhanyu Chen Xiaokun Fang Xueshun Yuan Yingying Zhang Huiying Li Ying Zhou Xiyan Cui |
| author_facet | Zhanyu Chen Xiaokun Fang Xueshun Yuan Yingying Zhang Huiying Li Ying Zhou Xiyan Cui |
| author_sort | Zhanyu Chen |
| collection | DOAJ |
| description | Soybean (<i>Glycine max</i>) is one of the important oil crops worldwide. In recent years, environmental stresses such as drought and soil salinization have severely deteriorated soybean yield and quality. We investigated the overexpression of the transcription factor GmTGA15 in response to drought stress in transgenic soybean hairy roots and Arabidopsis plants. The results of quantitative real time polymerase chain reaction (qRT-PCR) analyses showed that <i>GmTGA15</i> was greatly induced by salt, PEG6000, salicylic acid (SA), gibberellic acid (GA), abscisic acid (ABA), and methyl jasmonate (MeJA) in soybean. In response to drought stress, the contents of both chlorophyll and proline were significantly increased, while the content of malondialdehyde (MDA) was significantly decreased in the soybean hairy roots with the overexpression of <i>GmTGA15</i> in comparison to wild type (WT). Under the simulated drought conditions, the transgenic Arabidopsis plants showed significantly longer roots and lower mortality than that of the wild type. These results suggest that GmTGA15 promotes tolerance to drought stress in both soybean and Arabidopsis plants. This study provides the scientific evidence for further functional analysis of soybean TGA transcription factors in drought stress and the breeding of drought-resistance crops. |
| first_indexed | 2024-03-09T04:30:42Z |
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| id | doaj.art-3f1c443383d0437ba94ceb9c9721dc46 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T04:30:42Z |
| publishDate | 2021-01-01 |
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| series | Agronomy |
| spelling | doaj.art-3f1c443383d0437ba94ceb9c9721dc462023-12-03T13:36:00ZengMDPI AGAgronomy2073-43952021-01-0111117010.3390/agronomy11010170Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis PlantsZhanyu Chen0Xiaokun Fang1Xueshun Yuan2Yingying Zhang3Huiying Li4Ying Zhou5Xiyan Cui6College of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaSoybean (<i>Glycine max</i>) is one of the important oil crops worldwide. In recent years, environmental stresses such as drought and soil salinization have severely deteriorated soybean yield and quality. We investigated the overexpression of the transcription factor GmTGA15 in response to drought stress in transgenic soybean hairy roots and Arabidopsis plants. The results of quantitative real time polymerase chain reaction (qRT-PCR) analyses showed that <i>GmTGA15</i> was greatly induced by salt, PEG6000, salicylic acid (SA), gibberellic acid (GA), abscisic acid (ABA), and methyl jasmonate (MeJA) in soybean. In response to drought stress, the contents of both chlorophyll and proline were significantly increased, while the content of malondialdehyde (MDA) was significantly decreased in the soybean hairy roots with the overexpression of <i>GmTGA15</i> in comparison to wild type (WT). Under the simulated drought conditions, the transgenic Arabidopsis plants showed significantly longer roots and lower mortality than that of the wild type. These results suggest that GmTGA15 promotes tolerance to drought stress in both soybean and Arabidopsis plants. This study provides the scientific evidence for further functional analysis of soybean TGA transcription factors in drought stress and the breeding of drought-resistance crops.https://www.mdpi.com/2073-4395/11/1/170soybean hairy roots<i>Arabidopsis</i>TGA transcription factorabiotic stressdrought tolerance |
| spellingShingle | Zhanyu Chen Xiaokun Fang Xueshun Yuan Yingying Zhang Huiying Li Ying Zhou Xiyan Cui Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis Plants Agronomy soybean hairy roots <i>Arabidopsis</i> TGA transcription factor abiotic stress drought tolerance |
| title | Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis Plants |
| title_full | Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis Plants |
| title_fullStr | Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis Plants |
| title_full_unstemmed | Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis Plants |
| title_short | Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis Plants |
| title_sort | overexpression of transcription factor gmtga15 enhances drought tolerance in transgenic soybean hairy roots and arabidopsis plants |
| topic | soybean hairy roots <i>Arabidopsis</i> TGA transcription factor abiotic stress drought tolerance |
| url | https://www.mdpi.com/2073-4395/11/1/170 |
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