Piceatannol Ameliorates Hepatic Oxidative Damage and Mitochondrial Dysfunction of Weaned Piglets Challenged with Diquat
The liver is an organ that produces large amounts of reactive oxygen species (ROS). Human infants or piglets are prone to oxidative damage due to their uncompleted development of the antioxidant system, causing liver disease. Piceatannol (PIC) has been found to have significant antioxidant effects....
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/7/1239 |
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| author | Peilu Jia Shuli Ji Hao Zhang Yanan Chen Tian Wang |
| author_facet | Peilu Jia Shuli Ji Hao Zhang Yanan Chen Tian Wang |
| author_sort | Peilu Jia |
| collection | DOAJ |
| description | The liver is an organ that produces large amounts of reactive oxygen species (ROS). Human infants or piglets are prone to oxidative damage due to their uncompleted development of the antioxidant system, causing liver disease. Piceatannol (PIC) has been found to have significant antioxidant effects. The aim of this experiment was to investigate the effects of PIC on the liver in piglets experiencing oxidative stress caused by diquat (DQ). After weaning, 54 male piglets (Duroc × [Landrace × Yorkshire]) were selected and randomly divided into three treatment groups: the CON group, the DQ-CON group, and the DQ-PIC group. The two challenged groups were injected with DQ and then orally administrated either PIC or another vehicle solution, while the control group was given sterile saline injections and an orally administrated vehicle solution. Compared to the results of the CON group, DQ increased the percentage of apoptosis cells in the liver, also decreased the amount of reduced glutathione (GSH) and increased the concentration of malondialdehyde (MDA). In addition, the adenosine triphosphate (ATP) production, activities of mitochondrial complex I, II, III, and V, and the protein expression level of sirtuin 1 (SIRT1) were inhibited by DQ. Furthermore, PIC supplementation inhibited the apoptosis of hepatic cells caused by DQ. PIC also decreased MDA levels and increased the amount of GSH. Piglets given PIC supplementation exhibited increased activities of mitochondrial complex I, II, III, and V, the protein expression level of SIRT1, and the ATP production in the liver. In conclusion, PIC affected the liver of piglets by improving redox status, preserving mitochondrial function, and preventing excessive apoptosis. |
| first_indexed | 2024-03-10T18:19:41Z |
| format | Article |
| id | doaj.art-3f1d3e5459c845408236327f19b499aa |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T18:19:41Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-3f1d3e5459c845408236327f19b499aa2023-11-20T07:26:16ZengMDPI AGAnimals2076-26152020-07-01107123910.3390/ani10071239Piceatannol Ameliorates Hepatic Oxidative Damage and Mitochondrial Dysfunction of Weaned Piglets Challenged with DiquatPeilu Jia0Shuli Ji1Hao Zhang2Yanan Chen3Tian Wang4College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, ChinaThe liver is an organ that produces large amounts of reactive oxygen species (ROS). Human infants or piglets are prone to oxidative damage due to their uncompleted development of the antioxidant system, causing liver disease. Piceatannol (PIC) has been found to have significant antioxidant effects. The aim of this experiment was to investigate the effects of PIC on the liver in piglets experiencing oxidative stress caused by diquat (DQ). After weaning, 54 male piglets (Duroc × [Landrace × Yorkshire]) were selected and randomly divided into three treatment groups: the CON group, the DQ-CON group, and the DQ-PIC group. The two challenged groups were injected with DQ and then orally administrated either PIC or another vehicle solution, while the control group was given sterile saline injections and an orally administrated vehicle solution. Compared to the results of the CON group, DQ increased the percentage of apoptosis cells in the liver, also decreased the amount of reduced glutathione (GSH) and increased the concentration of malondialdehyde (MDA). In addition, the adenosine triphosphate (ATP) production, activities of mitochondrial complex I, II, III, and V, and the protein expression level of sirtuin 1 (SIRT1) were inhibited by DQ. Furthermore, PIC supplementation inhibited the apoptosis of hepatic cells caused by DQ. PIC also decreased MDA levels and increased the amount of GSH. Piglets given PIC supplementation exhibited increased activities of mitochondrial complex I, II, III, and V, the protein expression level of SIRT1, and the ATP production in the liver. In conclusion, PIC affected the liver of piglets by improving redox status, preserving mitochondrial function, and preventing excessive apoptosis.https://www.mdpi.com/2076-2615/10/7/1239piceatannoldiquatoxidative stressmitochondrial functionapoptosispiglet |
| spellingShingle | Peilu Jia Shuli Ji Hao Zhang Yanan Chen Tian Wang Piceatannol Ameliorates Hepatic Oxidative Damage and Mitochondrial Dysfunction of Weaned Piglets Challenged with Diquat Animals piceatannol diquat oxidative stress mitochondrial function apoptosis piglet |
| title | Piceatannol Ameliorates Hepatic Oxidative Damage and Mitochondrial Dysfunction of Weaned Piglets Challenged with Diquat |
| title_full | Piceatannol Ameliorates Hepatic Oxidative Damage and Mitochondrial Dysfunction of Weaned Piglets Challenged with Diquat |
| title_fullStr | Piceatannol Ameliorates Hepatic Oxidative Damage and Mitochondrial Dysfunction of Weaned Piglets Challenged with Diquat |
| title_full_unstemmed | Piceatannol Ameliorates Hepatic Oxidative Damage and Mitochondrial Dysfunction of Weaned Piglets Challenged with Diquat |
| title_short | Piceatannol Ameliorates Hepatic Oxidative Damage and Mitochondrial Dysfunction of Weaned Piglets Challenged with Diquat |
| title_sort | piceatannol ameliorates hepatic oxidative damage and mitochondrial dysfunction of weaned piglets challenged with diquat |
| topic | piceatannol diquat oxidative stress mitochondrial function apoptosis piglet |
| url | https://www.mdpi.com/2076-2615/10/7/1239 |
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