Flavonols Induced by 5-Aminolevulinic Acid Are Involved in Regulation of Stomatal Opening in Apple Leaves
Flavonols, the main flavonoids in plant leaves, have newly been proposed as a H2O2 scavenger in guard cells. However, whether 5-aminolevulinic acid (5-ALA) is involved in regulating stomatal movement of apple (Malus × domestica Borkh. ‘Fuji’) leaves through flavonol accumulation remains unclear. In...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2016-11-01
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| Series: | Horticultural Plant Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014117300316 |
| _version_ | 1797203629071925248 |
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| author | Longbo LIU Lijun XIONG Yuyan AN Jie ZHENG Liangju WANG |
| author_facet | Longbo LIU Lijun XIONG Yuyan AN Jie ZHENG Liangju WANG |
| author_sort | Longbo LIU |
| collection | DOAJ |
| description | Flavonols, the main flavonoids in plant leaves, have newly been proposed as a H2O2 scavenger in guard cells. However, whether 5-aminolevulinic acid (5-ALA) is involved in regulating stomatal movement of apple (Malus × domestica Borkh. ‘Fuji’) leaves through flavonol accumulation remains unclear. In this study, using diphenylboric acid 2-amino ethyl ester (DPBA, a flavonol fluorescent dye) and a laser scanning confocal microscope, we observed that 5-ALA pretreatment significantly improved flavonol accumulation in guard cells of apple leaves, especially around the nucleus. Then we demonstrated that 5-ALA pretreatment inhibited ABA-induced stomatal closure via decreasing reaction oxygen species (ROS) accumulation in guard cells. Pretreatment with quercetin or kaempferol, two main kinds of flavonols in plants, also inhibited ABA-induced stomatal closure by decreasing ROS content in the guard cells. Furthermore, exogenous flavonols could suppress H2O2-induced stomatal closure in apple leaves. Taken together, we conclude that ALA-induced flavonol accumulation in guard cells is involved in the inhibitory effect of ALA on ABA-induced ROS accumulation and stomatal closure in apple leaves. |
| first_indexed | 2024-04-24T08:22:22Z |
| format | Article |
| id | doaj.art-3f1e64f0947a4e48b1733f1846fe97a6 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:22:22Z |
| publishDate | 2016-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-3f1e64f0947a4e48b1733f1846fe97a62024-04-17T00:32:33ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412016-11-012632333010.1016/j.hpj.2017.02.002Flavonols Induced by 5-Aminolevulinic Acid Are Involved in Regulation of Stomatal Opening in Apple LeavesLongbo LIULijun XIONGYuyan ANJie ZHENGLiangju WANGFlavonols, the main flavonoids in plant leaves, have newly been proposed as a H2O2 scavenger in guard cells. However, whether 5-aminolevulinic acid (5-ALA) is involved in regulating stomatal movement of apple (Malus × domestica Borkh. ‘Fuji’) leaves through flavonol accumulation remains unclear. In this study, using diphenylboric acid 2-amino ethyl ester (DPBA, a flavonol fluorescent dye) and a laser scanning confocal microscope, we observed that 5-ALA pretreatment significantly improved flavonol accumulation in guard cells of apple leaves, especially around the nucleus. Then we demonstrated that 5-ALA pretreatment inhibited ABA-induced stomatal closure via decreasing reaction oxygen species (ROS) accumulation in guard cells. Pretreatment with quercetin or kaempferol, two main kinds of flavonols in plants, also inhibited ABA-induced stomatal closure by decreasing ROS content in the guard cells. Furthermore, exogenous flavonols could suppress H2O2-induced stomatal closure in apple leaves. Taken together, we conclude that ALA-induced flavonol accumulation in guard cells is involved in the inhibitory effect of ALA on ABA-induced ROS accumulation and stomatal closure in apple leaves.http://www.sciencedirect.com/science/article/pii/S2468014117300316apple (Malus × domestica Borkh. ‘Fuji’)ABA5-ALAflavonolsstomatal apertureguard cell |
| spellingShingle | Longbo LIU Lijun XIONG Yuyan AN Jie ZHENG Liangju WANG Flavonols Induced by 5-Aminolevulinic Acid Are Involved in Regulation of Stomatal Opening in Apple Leaves Horticultural Plant Journal apple (Malus × domestica Borkh. ‘Fuji’) ABA 5-ALA flavonols stomatal aperture guard cell |
| title | Flavonols Induced by 5-Aminolevulinic Acid Are Involved in Regulation of Stomatal Opening in Apple Leaves |
| title_full | Flavonols Induced by 5-Aminolevulinic Acid Are Involved in Regulation of Stomatal Opening in Apple Leaves |
| title_fullStr | Flavonols Induced by 5-Aminolevulinic Acid Are Involved in Regulation of Stomatal Opening in Apple Leaves |
| title_full_unstemmed | Flavonols Induced by 5-Aminolevulinic Acid Are Involved in Regulation of Stomatal Opening in Apple Leaves |
| title_short | Flavonols Induced by 5-Aminolevulinic Acid Are Involved in Regulation of Stomatal Opening in Apple Leaves |
| title_sort | flavonols induced by 5 aminolevulinic acid are involved in regulation of stomatal opening in apple leaves |
| topic | apple (Malus × domestica Borkh. ‘Fuji’) ABA 5-ALA flavonols stomatal aperture guard cell |
| url | http://www.sciencedirect.com/science/article/pii/S2468014117300316 |
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