Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietin
Mathematical modeling of metabolism meets several important applications in the context of bioprocess engineering, such as the interpretation of cell physiology. Metabolic Flux Analysis, one of the tools of this discipline, was used in the present work to characterize the biosynthesis of recombinant...
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| Format: | Article |
| Language: | English |
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Elfos Scientiae
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| Series: | Biotecnología Aplicada |
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| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522012000400004&lng=en&tlng=en |
| _version_ | 1819022502486081536 |
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| author | Osmán Fernández Julio C Dustet Ernesto Chico |
| author_facet | Osmán Fernández Julio C Dustet Ernesto Chico |
| author_sort | Osmán Fernández |
| collection | DOAJ |
| description | Mathematical modeling of metabolism meets several important applications in the context of bioprocess engineering, such as the interpretation of cell physiology. Metabolic Flux Analysis, one of the tools of this discipline, was used in the present work to characterize the biosynthesis of recombinant human erythropoietin in CHO cells. In order to apply this method, we built a matrix of stoichiometric numbers representing the major metabolic pathways for the generation of energy and the synthesis of essential precursors for product and biomass accumulation. Equations representing the biosynthesis of recombinant human erythropoietin and the growth of CHO were also derived, conferring an advantage to the proposed model over other existing designs. The dimensions of the obtained matrix were 47 × 44, with a rank of 44 and a condition number of 83; therefore, the model has a unique solution and is not sensitive. The metabolic flux map obtained by solving the mathematical model using experimental data showed results consistent with the known biochemistry of CHO cells and with the findings of other reports on this and other mammalian cell lines. The general steps of the methodology used to obtain the proposed mathematical model are also outlined. |
| first_indexed | 2024-12-21T04:24:01Z |
| format | Article |
| id | doaj.art-3f1f1c0286164af5b8a22bbd7538f2a3 |
| institution | Directory Open Access Journal |
| issn | 1027-2852 |
| language | English |
| last_indexed | 2024-12-21T04:24:01Z |
| publisher | Elfos Scientiae |
| record_format | Article |
| series | Biotecnología Aplicada |
| spelling | doaj.art-3f1f1c0286164af5b8a22bbd7538f2a32022-12-21T19:16:06ZengElfos ScientiaeBiotecnología Aplicada1027-2852294246252S1027-28522012000400004Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietinOsmán Fernández0Julio C Dustet1Ernesto Chico2Centro de Inmunología MolecularInstituto Superior Politécnico José Antonio EcheverríaCentro de Inmunología MolecularMathematical modeling of metabolism meets several important applications in the context of bioprocess engineering, such as the interpretation of cell physiology. Metabolic Flux Analysis, one of the tools of this discipline, was used in the present work to characterize the biosynthesis of recombinant human erythropoietin in CHO cells. In order to apply this method, we built a matrix of stoichiometric numbers representing the major metabolic pathways for the generation of energy and the synthesis of essential precursors for product and biomass accumulation. Equations representing the biosynthesis of recombinant human erythropoietin and the growth of CHO were also derived, conferring an advantage to the proposed model over other existing designs. The dimensions of the obtained matrix were 47 × 44, with a rank of 44 and a condition number of 83; therefore, the model has a unique solution and is not sensitive. The metabolic flux map obtained by solving the mathematical model using experimental data showed results consistent with the known biochemistry of CHO cells and with the findings of other reports on this and other mammalian cell lines. The general steps of the methodology used to obtain the proposed mathematical model are also outlined.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522012000400004&lng=en&tlng=enmetabolic flux analysismetabolismmathematical modelchoepo |
| spellingShingle | Osmán Fernández Julio C Dustet Ernesto Chico Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietin Biotecnología Aplicada metabolic flux analysis metabolism mathematical model cho epo |
| title | Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietin |
| title_full | Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietin |
| title_fullStr | Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietin |
| title_full_unstemmed | Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietin |
| title_short | Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietin |
| title_sort | mathematical model for the application of metabolic flux analysis to cho cells producing recombinant human erythropoietin |
| topic | metabolic flux analysis metabolism mathematical model cho epo |
| url | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522012000400004&lng=en&tlng=en |
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