Molecular Epidemiology of Stenotrophomonas maltophilia Strains Isolated from Bacteremia in Hospitalized Children
Objectives: The aim of this study was to determine the phenotypic and genotypic characteristics of Stenotrophomonas maltophilia isolates obtained from blood culture samples of pediatric patients hospitalized in Borujerd and Hamadan hospitals in western Iran. Methods: Oxidase-negative isolates were c...
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| Format: | Article |
| Language: | English |
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Oman Medical Specialty Board
2023-05-01
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| Series: | Oman Medical Journal |
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| Online Access: | https://omjournal.org/articleDetails.aspx?coType=1&aId=3422 |
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| author | Hadis Divakan Mohammad Yousef Alikhani Leili Shokoohizadeh |
| author_facet | Hadis Divakan Mohammad Yousef Alikhani Leili Shokoohizadeh |
| author_sort | Hadis Divakan |
| collection | DOAJ |
| description | Objectives: The aim of this study was to determine the phenotypic and genotypic characteristics of Stenotrophomonas maltophilia isolates obtained from blood culture samples of pediatric patients hospitalized in Borujerd and Hamadan hospitals in western Iran. Methods: Oxidase-negative isolates were collected from the blood cultures of pediatric patients. S. maltophilia isolates were identified and confirmed by routine microbiological and molecular testing. Antibiotic susceptibility of the isolates was determined. The phenotypic and genotypic biofilm-forming ability of the isolates were investigated. Molecular typing of all isolates was performed by repetitive element sequence-based polymerase chain reaction. Results: Out of 450 oxidase-negative bacilli, 72 (16.0%) were identified as S. maltophilia isolates. Biofilm assay results showed strong biofilm formation in 19 (26.4%) isolates, moderate in 38 (52.8%), weak in 10 (13.9%), and no biofilm formation in five (6.9%) isolates. Biofilm-associated genes rmlA, rpfF, and spgM were detected respectively in 59 (81.9%), 54 (75.0%), and 72 (100%) of isolates. Antimicrobial susceptibility testing showed that 67 (93.1%) isolates were sensitive to trimethoprim-sulfamethoxazole. All isolates were sensitive to levofloxacin and resistant to ceftazidime. The S. maltophilia isolates were grouped into 14 different types of repetitive sequence by repetitive element sequence-based polymerase chain reaction analysis. Conclusions: The results of this study indicate that S. maltophilia should be considered an important opportunistic pathogen in pediatric units. Different genotypes of S. maltophilia with the ability to form a biofilm (an important virulence factor) were circulating in the hospitals investigated. Levofloxacin and trimethoprim-sulfamethoxazole are recommended to treat S. maltophilia infections. |
| first_indexed | 2024-03-13T04:23:41Z |
| format | Article |
| id | doaj.art-3f204e3d493546a081179541ea33ca6e |
| institution | Directory Open Access Journal |
| issn | 1999-768X 2070-5204 |
| language | English |
| last_indexed | 2024-03-13T04:23:41Z |
| publishDate | 2023-05-01 |
| publisher | Oman Medical Specialty Board |
| record_format | Article |
| series | Oman Medical Journal |
| spelling | doaj.art-3f204e3d493546a081179541ea33ca6e2023-06-20T07:57:35ZengOman Medical Specialty BoardOman Medical Journal1999-768X2070-52042023-05-01383e505e50510.5001/omj.2023.76Molecular Epidemiology of Stenotrophomonas maltophilia Strains Isolated from Bacteremia in Hospitalized ChildrenHadis Divakan0Mohammad Yousef Alikhani1Leili Shokoohizadeh2Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IranInfectious Disease Research Center, Hamadan University of Medical Sciences, Hamadan, IranInfectious Disease Research Center, Hamadan University of Medical Sciences, Hamadan, IranObjectives: The aim of this study was to determine the phenotypic and genotypic characteristics of Stenotrophomonas maltophilia isolates obtained from blood culture samples of pediatric patients hospitalized in Borujerd and Hamadan hospitals in western Iran. Methods: Oxidase-negative isolates were collected from the blood cultures of pediatric patients. S. maltophilia isolates were identified and confirmed by routine microbiological and molecular testing. Antibiotic susceptibility of the isolates was determined. The phenotypic and genotypic biofilm-forming ability of the isolates were investigated. Molecular typing of all isolates was performed by repetitive element sequence-based polymerase chain reaction. Results: Out of 450 oxidase-negative bacilli, 72 (16.0%) were identified as S. maltophilia isolates. Biofilm assay results showed strong biofilm formation in 19 (26.4%) isolates, moderate in 38 (52.8%), weak in 10 (13.9%), and no biofilm formation in five (6.9%) isolates. Biofilm-associated genes rmlA, rpfF, and spgM were detected respectively in 59 (81.9%), 54 (75.0%), and 72 (100%) of isolates. Antimicrobial susceptibility testing showed that 67 (93.1%) isolates were sensitive to trimethoprim-sulfamethoxazole. All isolates were sensitive to levofloxacin and resistant to ceftazidime. The S. maltophilia isolates were grouped into 14 different types of repetitive sequence by repetitive element sequence-based polymerase chain reaction analysis. Conclusions: The results of this study indicate that S. maltophilia should be considered an important opportunistic pathogen in pediatric units. Different genotypes of S. maltophilia with the ability to form a biofilm (an important virulence factor) were circulating in the hospitals investigated. Levofloxacin and trimethoprim-sulfamethoxazole are recommended to treat S. maltophilia infections.https://omjournal.org/articleDetails.aspx?coType=1&aId=3422stenotrophomonas maltophiliapediatricsbacteremiaantibiotic resistancebiofilmmolecular typingiran |
| spellingShingle | Hadis Divakan Mohammad Yousef Alikhani Leili Shokoohizadeh Molecular Epidemiology of Stenotrophomonas maltophilia Strains Isolated from Bacteremia in Hospitalized Children Oman Medical Journal stenotrophomonas maltophilia pediatrics bacteremia antibiotic resistance biofilm molecular typing iran |
| title | Molecular Epidemiology of Stenotrophomonas maltophilia Strains Isolated from Bacteremia in Hospitalized Children |
| title_full | Molecular Epidemiology of Stenotrophomonas maltophilia Strains Isolated from Bacteremia in Hospitalized Children |
| title_fullStr | Molecular Epidemiology of Stenotrophomonas maltophilia Strains Isolated from Bacteremia in Hospitalized Children |
| title_full_unstemmed | Molecular Epidemiology of Stenotrophomonas maltophilia Strains Isolated from Bacteremia in Hospitalized Children |
| title_short | Molecular Epidemiology of Stenotrophomonas maltophilia Strains Isolated from Bacteremia in Hospitalized Children |
| title_sort | molecular epidemiology of stenotrophomonas maltophilia strains isolated from bacteremia in hospitalized children |
| topic | stenotrophomonas maltophilia pediatrics bacteremia antibiotic resistance biofilm molecular typing iran |
| url | https://omjournal.org/articleDetails.aspx?coType=1&aId=3422 |
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