Oxidative Stress in Far Eastern Mussel <i>Mytilus trossulus</i> (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs)
The ingress of nanoparticles of metal oxides and microfragments of synthetic polymers (microplastics) into a marine environment causes unpredictable consequences. The effects of such particles cannot be predicted due to a lack of ecotoxicological information. In this research, a series of laboratory...
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MDPI AG
2022-05-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/10/5/707 |
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| author | Nadezda Vladimirovna Dovzhenko Victor Pavlovich Chelomin Andrey Alexandrovich Mazur Sergey Petrovich Kukla Valentina Vladimirovna Slobodskova Aleksandra Anatolievna Istomina Avianna Fayazovna Zhukovskaya |
| author_facet | Nadezda Vladimirovna Dovzhenko Victor Pavlovich Chelomin Andrey Alexandrovich Mazur Sergey Petrovich Kukla Valentina Vladimirovna Slobodskova Aleksandra Anatolievna Istomina Avianna Fayazovna Zhukovskaya |
| author_sort | Nadezda Vladimirovna Dovzhenko |
| collection | DOAJ |
| description | The ingress of nanoparticles of metal oxides and microfragments of synthetic polymers (microplastics) into a marine environment causes unpredictable consequences. The effects of such particles cannot be predicted due to a lack of ecotoxicological information. In this research, a series of laboratory experiments were conducted on the combined effects of CuO-nanoparticles (CuO-NPs) and polystyrene microspheres (µPSs) on the development of oxidative stress processes in the marine filter-feeder mollusk <i>Mytilus trossulus</i>. Biomarkers of oxidative stress, including the lysosome membrane stability of hematocytes (LMS), the index of antioxidant activity (IAA), the levels of malonaldehyde (MDA) and protein carbonyls (PCs), and DNA damage in digestive gland cells, were measured after 5 days of exposure. Based on a battery of biochemical markers, it was shown that oxidative stress was induced at varying degrees in the experimental mollusks when exposed to CuO-NPs and µPSs both separately and in combination. In contrast, the single-treatment effect on the lysosomal membrane was enhanced by the combined CuO-NPs and µPSs (from 77.14 ± 8.56 to 42 ± 4.26 min). In addition, exposure to both the compounds alone and in combination decreased the IAA (from 22.87 ± 1.25, to 19.55 ± 0.21, 10.73 ± 0.53, and 12.06 ± 1.62 nM/mg protein, respectively). The PC level significantly increased only after CuO-NP exposure (from 0.496 ± 0.02 to 0.838 ± 0.03 μM/mg protein). Furthermore, the results showed that the investigated particles, both alone and in combination, promoted DNA damage in digestive gland cells (from 2.02 ± 0.52 to 5.15 ± 0.37, 18.29 ± 2.14, and 10.72 ± 2.53%, respectively), indicating that these compounds are genotoxic. Overall, the results obtained suggest that oxidative stress is the leading factor in the negative effects of CuO-NPs and µPSs. Considering the exceptional role of genome integrity in the functioning of biological systems, the revealed damages in the DNA molecule structure should be attributed to the most important manifestations of the toxicity of these two forms of marine pollution. |
| first_indexed | 2024-03-10T03:37:26Z |
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| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-10T03:37:26Z |
| publishDate | 2022-05-01 |
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| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-3f2080f51d0d4e8696dcc2a514a93d742023-11-23T11:40:40ZengMDPI AGJournal of Marine Science and Engineering2077-13122022-05-0110570710.3390/jmse10050707Oxidative Stress in Far Eastern Mussel <i>Mytilus trossulus</i> (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs)Nadezda Vladimirovna Dovzhenko0Victor Pavlovich Chelomin1Andrey Alexandrovich Mazur2Sergey Petrovich Kukla3Valentina Vladimirovna Slobodskova4Aleksandra Anatolievna Istomina5Avianna Fayazovna Zhukovskaya6Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaThe ingress of nanoparticles of metal oxides and microfragments of synthetic polymers (microplastics) into a marine environment causes unpredictable consequences. The effects of such particles cannot be predicted due to a lack of ecotoxicological information. In this research, a series of laboratory experiments were conducted on the combined effects of CuO-nanoparticles (CuO-NPs) and polystyrene microspheres (µPSs) on the development of oxidative stress processes in the marine filter-feeder mollusk <i>Mytilus trossulus</i>. Biomarkers of oxidative stress, including the lysosome membrane stability of hematocytes (LMS), the index of antioxidant activity (IAA), the levels of malonaldehyde (MDA) and protein carbonyls (PCs), and DNA damage in digestive gland cells, were measured after 5 days of exposure. Based on a battery of biochemical markers, it was shown that oxidative stress was induced at varying degrees in the experimental mollusks when exposed to CuO-NPs and µPSs both separately and in combination. In contrast, the single-treatment effect on the lysosomal membrane was enhanced by the combined CuO-NPs and µPSs (from 77.14 ± 8.56 to 42 ± 4.26 min). In addition, exposure to both the compounds alone and in combination decreased the IAA (from 22.87 ± 1.25, to 19.55 ± 0.21, 10.73 ± 0.53, and 12.06 ± 1.62 nM/mg protein, respectively). The PC level significantly increased only after CuO-NP exposure (from 0.496 ± 0.02 to 0.838 ± 0.03 μM/mg protein). Furthermore, the results showed that the investigated particles, both alone and in combination, promoted DNA damage in digestive gland cells (from 2.02 ± 0.52 to 5.15 ± 0.37, 18.29 ± 2.14, and 10.72 ± 2.53%, respectively), indicating that these compounds are genotoxic. Overall, the results obtained suggest that oxidative stress is the leading factor in the negative effects of CuO-NPs and µPSs. Considering the exceptional role of genome integrity in the functioning of biological systems, the revealed damages in the DNA molecule structure should be attributed to the most important manifestations of the toxicity of these two forms of marine pollution.https://www.mdpi.com/2077-1312/10/5/707microplasticsnanoparticlesgenotoxicitybivalveoxidative stress |
| spellingShingle | Nadezda Vladimirovna Dovzhenko Victor Pavlovich Chelomin Andrey Alexandrovich Mazur Sergey Petrovich Kukla Valentina Vladimirovna Slobodskova Aleksandra Anatolievna Istomina Avianna Fayazovna Zhukovskaya Oxidative Stress in Far Eastern Mussel <i>Mytilus trossulus</i> (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs) Journal of Marine Science and Engineering microplastics nanoparticles genotoxicity bivalve oxidative stress |
| title | Oxidative Stress in Far Eastern Mussel <i>Mytilus trossulus</i> (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs) |
| title_full | Oxidative Stress in Far Eastern Mussel <i>Mytilus trossulus</i> (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs) |
| title_fullStr | Oxidative Stress in Far Eastern Mussel <i>Mytilus trossulus</i> (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs) |
| title_full_unstemmed | Oxidative Stress in Far Eastern Mussel <i>Mytilus trossulus</i> (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs) |
| title_short | Oxidative Stress in Far Eastern Mussel <i>Mytilus trossulus</i> (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs) |
| title_sort | oxidative stress in far eastern mussel i mytilus trossulus i gould 1850 exposed to combined polystyrene microspheres µpss and cuo nanoparticles cuo nps |
| topic | microplastics nanoparticles genotoxicity bivalve oxidative stress |
| url | https://www.mdpi.com/2077-1312/10/5/707 |
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