Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to <i>Meloidogyne incognita</i>
<i>M. incognita</i> is a major parasitic plant disease in watermelon production, causing serious economic losses. Although there are many studies on root-knot nematode, the resistance mechanism is still unclear. In this study, in order to fully understand the mechanism of watermelon resi...
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MDPI AG
2022-07-01
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| author | Yingchun Zhu Gaopeng Yuan Renzong Zhao Guolin An Weihua Li Wenjing Si Junpu Liu Dexi Sun |
| author_facet | Yingchun Zhu Gaopeng Yuan Renzong Zhao Guolin An Weihua Li Wenjing Si Junpu Liu Dexi Sun |
| author_sort | Yingchun Zhu |
| collection | DOAJ |
| description | <i>M. incognita</i> is a major parasitic plant disease in watermelon production, causing serious economic losses. Although there are many studies on root-knot nematode, the resistance mechanism is still unclear. In this study, in order to fully understand the mechanism of watermelon resistance to root-knot nematode, the relatively strongly resistant ‘Hongzi watermelon’ variety and the susceptible ‘M16’ watermelon variety were used as materials, combined with RNA sequencing (RNA-seq), to analyze the expression abundance of resistant and susceptible varieties at 0, 2, 8 and 15 days post-infection (DPI) by <i>M. incognita.</i> The number of differentially expressed genes (DEGs) in the four comparison groups (A0_B0, A1_B1, A2_B2 and A3_B3) was 3645, 2306, 4449 and 2362, respectively, and there were 835 shared DEGs among them. GO annotation and KEGG pathway enrichment analysis showed that 835 DEGs were mainly involved in phenylpropane biosynthesis and carbon metabolism. Furthermore, lignin-biosynthesis-related genes (<i>4CL</i> (4-coumaric acid-CoA ligase), <i>C3H</i> (coumaric acid 3-hydroxylase), <i>CSE</i> (caffeoyl shikimate esterase), <i>COMT</i> (caffeic acid-O-methyltransferase), <i>CCR</i> (cinnamyl CoA reductase) and <i>PRX</i> (peroxidase)), defense-related proteins (UDP-glucoronosyl/UDP-glucosyl transferase, UGT84A13; salicylic acid binding protein, SABP2) and some transcription factors (TFs) were highlighted, which may be potential candidate genes for further analysis in the infection process of <i>M. incognita</i>. These results suggest that watermelon can achieve resistance to <i>M. incognita</i> by increasing the content of lignin and phenols in root or improving ROS level. These RNA-seq data provide new knowledge for future functional studies and will be helpful to further elucidate the molecular mechanism of resistance to <i>M. incognita</i> in watermelon. |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
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| spelling | doaj.art-3f209703954a4b2cb839fdf0412a86982023-11-30T21:17:57ZengMDPI AGLife2075-17292022-07-01127100310.3390/life12071003Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to <i>Meloidogyne incognita</i>Yingchun Zhu0Gaopeng Yuan1Renzong Zhao2Guolin An3Weihua Li4Wenjing Si5Junpu Liu6Dexi Sun7Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China<i>M. incognita</i> is a major parasitic plant disease in watermelon production, causing serious economic losses. Although there are many studies on root-knot nematode, the resistance mechanism is still unclear. In this study, in order to fully understand the mechanism of watermelon resistance to root-knot nematode, the relatively strongly resistant ‘Hongzi watermelon’ variety and the susceptible ‘M16’ watermelon variety were used as materials, combined with RNA sequencing (RNA-seq), to analyze the expression abundance of resistant and susceptible varieties at 0, 2, 8 and 15 days post-infection (DPI) by <i>M. incognita.</i> The number of differentially expressed genes (DEGs) in the four comparison groups (A0_B0, A1_B1, A2_B2 and A3_B3) was 3645, 2306, 4449 and 2362, respectively, and there were 835 shared DEGs among them. GO annotation and KEGG pathway enrichment analysis showed that 835 DEGs were mainly involved in phenylpropane biosynthesis and carbon metabolism. Furthermore, lignin-biosynthesis-related genes (<i>4CL</i> (4-coumaric acid-CoA ligase), <i>C3H</i> (coumaric acid 3-hydroxylase), <i>CSE</i> (caffeoyl shikimate esterase), <i>COMT</i> (caffeic acid-O-methyltransferase), <i>CCR</i> (cinnamyl CoA reductase) and <i>PRX</i> (peroxidase)), defense-related proteins (UDP-glucoronosyl/UDP-glucosyl transferase, UGT84A13; salicylic acid binding protein, SABP2) and some transcription factors (TFs) were highlighted, which may be potential candidate genes for further analysis in the infection process of <i>M. incognita</i>. These results suggest that watermelon can achieve resistance to <i>M. incognita</i> by increasing the content of lignin and phenols in root or improving ROS level. These RNA-seq data provide new knowledge for future functional studies and will be helpful to further elucidate the molecular mechanism of resistance to <i>M. incognita</i> in watermelon.https://www.mdpi.com/2075-1729/12/7/1003watermelonresistantsusceptiblecomparative transcriptome<i>Meloidogyne incognita</i>molecular mechanism |
| spellingShingle | Yingchun Zhu Gaopeng Yuan Renzong Zhao Guolin An Weihua Li Wenjing Si Junpu Liu Dexi Sun Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to <i>Meloidogyne incognita</i> Life watermelon resistant susceptible comparative transcriptome <i>Meloidogyne incognita</i> molecular mechanism |
| title | Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to <i>Meloidogyne incognita</i> |
| title_full | Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to <i>Meloidogyne incognita</i> |
| title_fullStr | Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to <i>Meloidogyne incognita</i> |
| title_full_unstemmed | Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to <i>Meloidogyne incognita</i> |
| title_short | Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to <i>Meloidogyne incognita</i> |
| title_sort | comparative transcriptome analysis reveals differential gene expression in resistant and susceptible watermelon varieties in response to i meloidogyne incognita i |
| topic | watermelon resistant susceptible comparative transcriptome <i>Meloidogyne incognita</i> molecular mechanism |
| url | https://www.mdpi.com/2075-1729/12/7/1003 |
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