Isolation and Characterization of the Primary Marmoset (<i>Callithrix jacchus</i>) Retinal Pigment Epithelial Cells
Marmosets have emerged as a valuable primate model in ophthalmic research due to their similarity to the human visual system and their potential for generating transgenic models to advance the development of therapies. In this study, we isolated and cultured primary retinal pigment epithelium (RPE)...
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MDPI AG
2023-06-01
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| Online Access: | https://www.mdpi.com/2073-4409/12/12/1644 |
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| author | Ha Young Jang Chang Sik Cho Young Mi Shin Jina Kwak Young Hoon Sung Byeong-Cheol Kang Jeong Hun Kim |
| author_facet | Ha Young Jang Chang Sik Cho Young Mi Shin Jina Kwak Young Hoon Sung Byeong-Cheol Kang Jeong Hun Kim |
| author_sort | Ha Young Jang |
| collection | DOAJ |
| description | Marmosets have emerged as a valuable primate model in ophthalmic research due to their similarity to the human visual system and their potential for generating transgenic models to advance the development of therapies. In this study, we isolated and cultured primary retinal pigment epithelium (RPE) cells from marmosets to investigate the mechanisms underlying RPE dysfunction in aging and age-related macular degeneration (AMD). We confirmed that our culture conditions and materials supported the formation of RPE monolayers with functional tight junctions that closely resembled the in vivo RPE. Since serum has been shown to induce epithelial–mesenchymal transition (EMT) in RPE cells, we compared the effects of fetal bovine serum (FBS) with serum-free supplements B27 on transepithelial electrical resistance (TER), cell proliferation, and morphological characteristics. Additionally, we assessed the age-related morphological changes of in vivo and primary RPE cells. Our results indicate that primary marmoset RPE cells exhibit in vivo-like characteristics, while cells obtained from an older donor show evidence of aging, including a failure to form a polarized monolayer, low TER, and delayed cell cycle. In conclusion, our primary marmoset RPE cells provide a reliable in vitro model for developing novel therapeutics for visual-threatening disorders such as AMD, which can be used before animal experiments using marmosets. |
| first_indexed | 2024-03-11T02:38:38Z |
| format | Article |
| id | doaj.art-3f24ae8daaf14efbb6a6d94828745712 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T02:38:38Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3f24ae8daaf14efbb6a6d948287457122023-11-18T09:43:52ZengMDPI AGCells2073-44092023-06-011212164410.3390/cells12121644Isolation and Characterization of the Primary Marmoset (<i>Callithrix jacchus</i>) Retinal Pigment Epithelial CellsHa Young Jang0Chang Sik Cho1Young Mi Shin2Jina Kwak3Young Hoon Sung4Byeong-Cheol Kang5Jeong Hun Kim6Fight Against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul 03082, Republic of KoreaFight Against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul 03082, Republic of KoreaFight Against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul 03082, Republic of KoreaGraduate School of Translational Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of KoreaAsan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of KoreaGraduate School of Translational Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of KoreaFight Against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul 03082, Republic of KoreaMarmosets have emerged as a valuable primate model in ophthalmic research due to their similarity to the human visual system and their potential for generating transgenic models to advance the development of therapies. In this study, we isolated and cultured primary retinal pigment epithelium (RPE) cells from marmosets to investigate the mechanisms underlying RPE dysfunction in aging and age-related macular degeneration (AMD). We confirmed that our culture conditions and materials supported the formation of RPE monolayers with functional tight junctions that closely resembled the in vivo RPE. Since serum has been shown to induce epithelial–mesenchymal transition (EMT) in RPE cells, we compared the effects of fetal bovine serum (FBS) with serum-free supplements B27 on transepithelial electrical resistance (TER), cell proliferation, and morphological characteristics. Additionally, we assessed the age-related morphological changes of in vivo and primary RPE cells. Our results indicate that primary marmoset RPE cells exhibit in vivo-like characteristics, while cells obtained from an older donor show evidence of aging, including a failure to form a polarized monolayer, low TER, and delayed cell cycle. In conclusion, our primary marmoset RPE cells provide a reliable in vitro model for developing novel therapeutics for visual-threatening disorders such as AMD, which can be used before animal experiments using marmosets.https://www.mdpi.com/2073-4409/12/12/1644marmosetretinal pigment epitheliumprimary cellsage-related macular degeneration |
| spellingShingle | Ha Young Jang Chang Sik Cho Young Mi Shin Jina Kwak Young Hoon Sung Byeong-Cheol Kang Jeong Hun Kim Isolation and Characterization of the Primary Marmoset (<i>Callithrix jacchus</i>) Retinal Pigment Epithelial Cells Cells marmoset retinal pigment epithelium primary cells age-related macular degeneration |
| title | Isolation and Characterization of the Primary Marmoset (<i>Callithrix jacchus</i>) Retinal Pigment Epithelial Cells |
| title_full | Isolation and Characterization of the Primary Marmoset (<i>Callithrix jacchus</i>) Retinal Pigment Epithelial Cells |
| title_fullStr | Isolation and Characterization of the Primary Marmoset (<i>Callithrix jacchus</i>) Retinal Pigment Epithelial Cells |
| title_full_unstemmed | Isolation and Characterization of the Primary Marmoset (<i>Callithrix jacchus</i>) Retinal Pigment Epithelial Cells |
| title_short | Isolation and Characterization of the Primary Marmoset (<i>Callithrix jacchus</i>) Retinal Pigment Epithelial Cells |
| title_sort | isolation and characterization of the primary marmoset i callithrix jacchus i retinal pigment epithelial cells |
| topic | marmoset retinal pigment epithelium primary cells age-related macular degeneration |
| url | https://www.mdpi.com/2073-4409/12/12/1644 |
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