Preliminary findings on the possible role of B-lymphocyte stimulator (BLyS) on diabetes-related periodontitis
Abstract The possible role of B-cell growth and differentiation-related cytokines on the pathogenesis of diabetes-related periodontitis has not been addressed so far. The aim of this study was to evaluate the effects of diabetes mellitus (DM) on the gene expression of proliferation-inducing ligand (...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Pesquisa Odontológica
2020-04-01
|
| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242020000100228&tlng=en |
| _version_ | 1818889314905358336 |
|---|---|
| author | Marx Haddley Ferreira DRUMOND Luciano Eduardo PUHL Poliana Mendes DUARTE Tamires Szeremeske de MIRANDA Juliana Trindade CLEMENTE-NAPIMOGA Daiane Cristina PERUZZO Elizabeth Ferreira MARTINEZ Marcelo Henrique NAPIMOGA |
| author_facet | Marx Haddley Ferreira DRUMOND Luciano Eduardo PUHL Poliana Mendes DUARTE Tamires Szeremeske de MIRANDA Juliana Trindade CLEMENTE-NAPIMOGA Daiane Cristina PERUZZO Elizabeth Ferreira MARTINEZ Marcelo Henrique NAPIMOGA |
| author_sort | Marx Haddley Ferreira DRUMOND |
| collection | DOAJ |
| description | Abstract The possible role of B-cell growth and differentiation-related cytokines on the pathogenesis of diabetes-related periodontitis has not been addressed so far. The aim of this study was to evaluate the effects of diabetes mellitus (DM) on the gene expression of proliferation-inducing ligand (APRIL) and B-lymphocyte stimulator (BLyS), two major cytokines associated to survival, differentiation and maturation of B cells in biopsies from gingival tissue with periodontitis. Gingival biopsies were obtained from subjects with periodontitis (n = 17), with periodontitis and DM (n = 19) as well as from periodontally and systemically healthy controls (n = 10). Gene expressions for APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were evaluated using qPCR. The expressions APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were all higher in both periodontitis groups when compared to the control group (p < 0.05). Furthermore, the expressions of BLyS, TRAP and RANKL were significantly higher in the subjects with periodontitis and DM when compared to those with periodontitis alone (p < 0.05). The mRNA levels of BLyS correlated positively with RANKL in the subjects with periodontitis and DM (p < 0.05). BLyS is overexpressed in periodontitis tissues of subjects with type 2 DM, suggesting a possible role of this cytokine on the pathogenesis DM-related periodontitis. |
| first_indexed | 2024-12-19T17:07:03Z |
| format | Article |
| id | doaj.art-3f24cbf3338d4d66995e2dae0b838f21 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-19T17:07:03Z |
| publishDate | 2020-04-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-3f24cbf3338d4d66995e2dae0b838f212022-12-21T20:13:08ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072020-04-013410.1590/1807-3107bor-2020.vol34.0038Preliminary findings on the possible role of B-lymphocyte stimulator (BLyS) on diabetes-related periodontitisMarx Haddley Ferreira DRUMONDhttps://orcid.org/0000-0001-7741-6301Luciano Eduardo PUHLhttps://orcid.org/0000-0003-0151-6861Poliana Mendes DUARTEhttps://orcid.org/0000-0001-8872-5943Tamires Szeremeske de MIRANDAhttps://orcid.org/0000-0003-2988-9205Juliana Trindade CLEMENTE-NAPIMOGAhttps://orcid.org/0000-0003-1068-3039Daiane Cristina PERUZZOhttps://orcid.org/0000-0002-4745-5526Elizabeth Ferreira MARTINEZhttps://orcid.org/0000-0002-4991-1185Marcelo Henrique NAPIMOGAhttps://orcid.org/0000-0003-4472-365XAbstract The possible role of B-cell growth and differentiation-related cytokines on the pathogenesis of diabetes-related periodontitis has not been addressed so far. The aim of this study was to evaluate the effects of diabetes mellitus (DM) on the gene expression of proliferation-inducing ligand (APRIL) and B-lymphocyte stimulator (BLyS), two major cytokines associated to survival, differentiation and maturation of B cells in biopsies from gingival tissue with periodontitis. Gingival biopsies were obtained from subjects with periodontitis (n = 17), with periodontitis and DM (n = 19) as well as from periodontally and systemically healthy controls (n = 10). Gene expressions for APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were evaluated using qPCR. The expressions APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were all higher in both periodontitis groups when compared to the control group (p < 0.05). Furthermore, the expressions of BLyS, TRAP and RANKL were significantly higher in the subjects with periodontitis and DM when compared to those with periodontitis alone (p < 0.05). The mRNA levels of BLyS correlated positively with RANKL in the subjects with periodontitis and DM (p < 0.05). BLyS is overexpressed in periodontitis tissues of subjects with type 2 DM, suggesting a possible role of this cytokine on the pathogenesis DM-related periodontitis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242020000100228&tlng=enPeriodontitisDiabetes MellitusInflammationCytokines |
| spellingShingle | Marx Haddley Ferreira DRUMOND Luciano Eduardo PUHL Poliana Mendes DUARTE Tamires Szeremeske de MIRANDA Juliana Trindade CLEMENTE-NAPIMOGA Daiane Cristina PERUZZO Elizabeth Ferreira MARTINEZ Marcelo Henrique NAPIMOGA Preliminary findings on the possible role of B-lymphocyte stimulator (BLyS) on diabetes-related periodontitis Brazilian Oral Research Periodontitis Diabetes Mellitus Inflammation Cytokines |
| title | Preliminary findings on the possible role of B-lymphocyte stimulator (BLyS) on diabetes-related periodontitis |
| title_full | Preliminary findings on the possible role of B-lymphocyte stimulator (BLyS) on diabetes-related periodontitis |
| title_fullStr | Preliminary findings on the possible role of B-lymphocyte stimulator (BLyS) on diabetes-related periodontitis |
| title_full_unstemmed | Preliminary findings on the possible role of B-lymphocyte stimulator (BLyS) on diabetes-related periodontitis |
| title_short | Preliminary findings on the possible role of B-lymphocyte stimulator (BLyS) on diabetes-related periodontitis |
| title_sort | preliminary findings on the possible role of b lymphocyte stimulator blys on diabetes related periodontitis |
| topic | Periodontitis Diabetes Mellitus Inflammation Cytokines |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242020000100228&tlng=en |
| work_keys_str_mv | AT marxhaddleyferreiradrumond preliminaryfindingsonthepossibleroleofblymphocytestimulatorblysondiabetesrelatedperiodontitis AT lucianoeduardopuhl preliminaryfindingsonthepossibleroleofblymphocytestimulatorblysondiabetesrelatedperiodontitis AT polianamendesduarte preliminaryfindingsonthepossibleroleofblymphocytestimulatorblysondiabetesrelatedperiodontitis AT tamiresszeremeskedemiranda preliminaryfindingsonthepossibleroleofblymphocytestimulatorblysondiabetesrelatedperiodontitis AT julianatrindadeclementenapimoga preliminaryfindingsonthepossibleroleofblymphocytestimulatorblysondiabetesrelatedperiodontitis AT daianecristinaperuzzo preliminaryfindingsonthepossibleroleofblymphocytestimulatorblysondiabetesrelatedperiodontitis AT elizabethferreiramartinez preliminaryfindingsonthepossibleroleofblymphocytestimulatorblysondiabetesrelatedperiodontitis AT marcelohenriquenapimoga preliminaryfindingsonthepossibleroleofblymphocytestimulatorblysondiabetesrelatedperiodontitis |