A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic Hordeum brevisubulatum
Halophytic Hordeum brevisubulatum is a perennial grass which has evolved many distinctive salt-adaptive mechanisms. Our previous studies indicated it could thrive under salt stress through maintaining better K+ and Na+ homeostasis. Stress-responsive HbCIPK2 can phosphorylate K+ channel HbVGKC1 and N...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.927253/full |
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| author | Ying Jiang Ying Jiang Haiwen Zhang Haiwen Zhang Yang Li Yang Li Congcong Chang Congcong Chang Yunxiao Wang Yunxiao Wang Hao Feng Hao Feng Ruifen Li Ruifen Li |
| author_facet | Ying Jiang Ying Jiang Haiwen Zhang Haiwen Zhang Yang Li Yang Li Congcong Chang Congcong Chang Yunxiao Wang Yunxiao Wang Hao Feng Hao Feng Ruifen Li Ruifen Li |
| author_sort | Ying Jiang |
| collection | DOAJ |
| description | Halophytic Hordeum brevisubulatum is a perennial grass which has evolved many distinctive salt-adaptive mechanisms. Our previous studies indicated it could thrive under salt stress through maintaining better K+ and Na+ homeostasis. Stress-responsive HbCIPK2 can phosphorylate K+ channel HbVGKC1 and Na+ transporter HbSOS1L to prevent Na+ accumulation and K+ reduction, hence pathway was not detected in glycophytic plants. In this study, we cloned the inducible promoter of HbCIPK2 by genome-walking, and identified a novel transcriptional regulator HbERF6 through yeast one-hybrid screening. HbERF6 functioned as a transcription factor which can bind to the GCC-box of the HbCIPK2 promoter to activate its expression. HbERF6 transgenic lines in Arabidopsis improved salt tolerance compared with wild type, and especially induced AtCIPK24 (SOS2) expression, resulting in K+/Na+ homeostasis to enhance salt tolerance. All the results confirmed the inducible function of HbERF6 for CIPK genes during salt tolerance. This regulatory network that integrates transcriptional regulation and post-translation modification will unravel a novel salt stress-responsive mechanism, highlighting the value and utilization of the halophytic resource. |
| first_indexed | 2024-04-13T14:38:42Z |
| format | Article |
| id | doaj.art-3f273dddb47847f1a9853b8c3ec6fb92 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T14:38:42Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3f273dddb47847f1a9853b8c3ec6fb922022-12-22T02:42:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.927253927253A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic Hordeum brevisubulatumYing Jiang0Ying Jiang1Haiwen Zhang2Haiwen Zhang3Yang Li4Yang Li5Congcong Chang6Congcong Chang7Yunxiao Wang8Yunxiao Wang9Hao Feng10Hao Feng11Ruifen Li12Ruifen Li13Agro-Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing, ChinaAgro-Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing, ChinaAgro-Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaCollege of Life Science, Hebei Normal University, Shijiazhuang, ChinaAgro-Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaCollege of Life Science, Hebei Normal University, Shijiazhuang, ChinaAgro-Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaCollege of Life Science, Hebei Normal University, Shijiazhuang, ChinaAgro-Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing, ChinaAgro-Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing, ChinaHalophytic Hordeum brevisubulatum is a perennial grass which has evolved many distinctive salt-adaptive mechanisms. Our previous studies indicated it could thrive under salt stress through maintaining better K+ and Na+ homeostasis. Stress-responsive HbCIPK2 can phosphorylate K+ channel HbVGKC1 and Na+ transporter HbSOS1L to prevent Na+ accumulation and K+ reduction, hence pathway was not detected in glycophytic plants. In this study, we cloned the inducible promoter of HbCIPK2 by genome-walking, and identified a novel transcriptional regulator HbERF6 through yeast one-hybrid screening. HbERF6 functioned as a transcription factor which can bind to the GCC-box of the HbCIPK2 promoter to activate its expression. HbERF6 transgenic lines in Arabidopsis improved salt tolerance compared with wild type, and especially induced AtCIPK24 (SOS2) expression, resulting in K+/Na+ homeostasis to enhance salt tolerance. All the results confirmed the inducible function of HbERF6 for CIPK genes during salt tolerance. This regulatory network that integrates transcriptional regulation and post-translation modification will unravel a novel salt stress-responsive mechanism, highlighting the value and utilization of the halophytic resource.https://www.frontiersin.org/articles/10.3389/fpls.2022.927253/fullHordeum brevisubulatumsalt tolerancetranscription factorCIPKhalophyte |
| spellingShingle | Ying Jiang Ying Jiang Haiwen Zhang Haiwen Zhang Yang Li Yang Li Congcong Chang Congcong Chang Yunxiao Wang Yunxiao Wang Hao Feng Hao Feng Ruifen Li Ruifen Li A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic Hordeum brevisubulatum Frontiers in Plant Science Hordeum brevisubulatum salt tolerance transcription factor CIPK halophyte |
| title | A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic Hordeum brevisubulatum |
| title_full | A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic Hordeum brevisubulatum |
| title_fullStr | A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic Hordeum brevisubulatum |
| title_full_unstemmed | A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic Hordeum brevisubulatum |
| title_short | A Novel Transcriptional Regulator HbERF6 Regulates the HbCIPK2-Coordinated Pathway Conferring Salt Tolerance in Halophytic Hordeum brevisubulatum |
| title_sort | novel transcriptional regulator hberf6 regulates the hbcipk2 coordinated pathway conferring salt tolerance in halophytic hordeum brevisubulatum |
| topic | Hordeum brevisubulatum salt tolerance transcription factor CIPK halophyte |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.927253/full |
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