Optimization Kerosene Bio-degradation by a Local Soil Bacterium Isolate Klebsiella pneumoniae Sp. pneumonia

Isolated Bacteria from the roots of barley were studied; two stages of processes Isolated and screening were applied in order to find the best bacteria to remove kerosene from soil. The active bacteria are isolated for kerosene degradation process. It has been found that Klebsiella pneumoniae sp....

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Main Authors: Mona Faiq Ali, Mohanad J. M-Ridha, Ahmed Hussein Taly
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2018-12-01
Series:Journal of Pure and Applied Microbiology
Subjects:
Online Access:https://microbiologyjournal.org/optimization-kerosene-bio-degradation-by-a-local-soil-bacterium-isolate-klebsiella-pneumoniae-sp-pneumonia/
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author Mona Faiq Ali
Mohanad J. M-Ridha
Ahmed Hussein Taly
author_facet Mona Faiq Ali
Mohanad J. M-Ridha
Ahmed Hussein Taly
author_sort Mona Faiq Ali
collection DOAJ
description Isolated Bacteria from the roots of barley were studied; two stages of processes Isolated and screening were applied in order to find the best bacteria to remove kerosene from soil. The active bacteria are isolated for kerosene degradation process. It has been found that Klebsiella pneumoniae sp. have the highest kerosene degradation which is 88.5%. The optimum conditions of kerosene degradation by Klebsiella pneumonia sp. are pH5, 48hr incubation period, 35°C temperature and 10000ppm the best kerosene concentration. The results 10000ppm showed that the maximum kerosene degradation can reach 99.58% after 48 h of incubation. Higher Kerosene degradation which was 99.83% was obtained at pH5. Kerosene degradation was found to be maximum at 35°C with 98.63%, where 10000ppm kerosene showed the highest degradation at 99.527%. The results indicate that the isolated Klebsiella pneumonia sp. is extremely efficient in degrading kerosene hydrocarbons.
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spelling doaj.art-3f2b730e380b42bb9bc96eca14ee01ee2022-12-21T23:24:47ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-12-011242049205710.22207/JPAM.12.4.41Optimization Kerosene Bio-degradation by a Local Soil Bacterium Isolate Klebsiella pneumoniae Sp. pneumoniaMona Faiq Ali0Mohanad J. M-Ridha1Ahmed Hussein Taly2Environmental Engineering college, University of Baghdad, Baghdad, Iraq. Environmental Engineering college, University of Baghdad, Baghdad, Iraq. College of Agriculture, University of Baghdad, Baghdad, Iraq.Isolated Bacteria from the roots of barley were studied; two stages of processes Isolated and screening were applied in order to find the best bacteria to remove kerosene from soil. The active bacteria are isolated for kerosene degradation process. It has been found that Klebsiella pneumoniae sp. have the highest kerosene degradation which is 88.5%. The optimum conditions of kerosene degradation by Klebsiella pneumonia sp. are pH5, 48hr incubation period, 35°C temperature and 10000ppm the best kerosene concentration. The results 10000ppm showed that the maximum kerosene degradation can reach 99.58% after 48 h of incubation. Higher Kerosene degradation which was 99.83% was obtained at pH5. Kerosene degradation was found to be maximum at 35°C with 98.63%, where 10000ppm kerosene showed the highest degradation at 99.527%. The results indicate that the isolated Klebsiella pneumonia sp. is extremely efficient in degrading kerosene hydrocarbons.https://microbiologyjournal.org/optimization-kerosene-bio-degradation-by-a-local-soil-bacterium-isolate-klebsiella-pneumoniae-sp-pneumonia/klebsiella pneumoniae sp.kerosene hydrocarbons degradationoptimum conditionsrhizodegradation
spellingShingle Mona Faiq Ali
Mohanad J. M-Ridha
Ahmed Hussein Taly
Optimization Kerosene Bio-degradation by a Local Soil Bacterium Isolate Klebsiella pneumoniae Sp. pneumonia
Journal of Pure and Applied Microbiology
klebsiella pneumoniae sp.
kerosene hydrocarbons degradation
optimum conditions
rhizodegradation
title Optimization Kerosene Bio-degradation by a Local Soil Bacterium Isolate Klebsiella pneumoniae Sp. pneumonia
title_full Optimization Kerosene Bio-degradation by a Local Soil Bacterium Isolate Klebsiella pneumoniae Sp. pneumonia
title_fullStr Optimization Kerosene Bio-degradation by a Local Soil Bacterium Isolate Klebsiella pneumoniae Sp. pneumonia
title_full_unstemmed Optimization Kerosene Bio-degradation by a Local Soil Bacterium Isolate Klebsiella pneumoniae Sp. pneumonia
title_short Optimization Kerosene Bio-degradation by a Local Soil Bacterium Isolate Klebsiella pneumoniae Sp. pneumonia
title_sort optimization kerosene bio degradation by a local soil bacterium isolate klebsiella pneumoniae sp pneumonia
topic klebsiella pneumoniae sp.
kerosene hydrocarbons degradation
optimum conditions
rhizodegradation
url https://microbiologyjournal.org/optimization-kerosene-bio-degradation-by-a-local-soil-bacterium-isolate-klebsiella-pneumoniae-sp-pneumonia/
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AT mohanadjmridha optimizationkerosenebiodegradationbyalocalsoilbacteriumisolateklebsiellapneumoniaesppneumonia
AT ahmedhusseintaly optimizationkerosenebiodegradationbyalocalsoilbacteriumisolateklebsiellapneumoniaesppneumonia