Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review
<b>: </b>Antifungal susceptibility testing is an important tool for managing patients with invasive fungal infections, as well as for epidemiological surveillance of emerging resistance. For routine testing in clinical microbiology laboratories, ready-to-use commercial methods are more p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/5/4/108 |
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| author | Eric Dannaoui Ana Espinel-Ingroff |
| author_facet | Eric Dannaoui Ana Espinel-Ingroff |
| author_sort | Eric Dannaoui |
| collection | DOAJ |
| description | <b>: </b>Antifungal susceptibility testing is an important tool for managing patients with invasive fungal infections, as well as for epidemiological surveillance of emerging resistance. For routine testing in clinical microbiology laboratories, ready-to-use commercial methods are more practical than homemade reference techniques. Among commercially available methods, the concentration gradient Etest strip technique is widely used. It combines an agar-based diffusion method with a dilution method that determinates a minimal inhibitory concentration (MIC) in µg/mL. Many studies have evaluated the agreement between the gradient strip method and the reference methods for both yeasts and filamentous fungi. This agreement has been variable depending on the antifungal, the species, and the incubation time. It has also been shown that the gradient strip method could be a valuable alternative for detection of emerging resistance (non-wild-type isolates) as Etest epidemiological cutoff values have been recently defined for several drug-species combinations. Furthermore, the Etest could be useful for direct antifungal susceptibility testing on blood samples and basic research studies (e.g., the evaluation of the in vitro activity of antifungal combinations). This review summarizes the available data on the performance and potential use of the gradient strip method. |
| first_indexed | 2024-12-10T04:12:12Z |
| format | Article |
| id | doaj.art-3f2bc354bf0240748a6946c31afa23c5 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-12-10T04:12:12Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-3f2bc354bf0240748a6946c31afa23c52022-12-22T02:02:42ZengMDPI AGJournal of Fungi2309-608X2019-11-015410810.3390/jof5040108jof5040108Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A ReviewEric Dannaoui0Ana Espinel-Ingroff1Paris-Descartes University, Faculty of Medicine, 75006 Paris, FranceVirginia Commonwealth University (VCU) Medical Center, Richmond, VA 23219, USA<b>: </b>Antifungal susceptibility testing is an important tool for managing patients with invasive fungal infections, as well as for epidemiological surveillance of emerging resistance. For routine testing in clinical microbiology laboratories, ready-to-use commercial methods are more practical than homemade reference techniques. Among commercially available methods, the concentration gradient Etest strip technique is widely used. It combines an agar-based diffusion method with a dilution method that determinates a minimal inhibitory concentration (MIC) in µg/mL. Many studies have evaluated the agreement between the gradient strip method and the reference methods for both yeasts and filamentous fungi. This agreement has been variable depending on the antifungal, the species, and the incubation time. It has also been shown that the gradient strip method could be a valuable alternative for detection of emerging resistance (non-wild-type isolates) as Etest epidemiological cutoff values have been recently defined for several drug-species combinations. Furthermore, the Etest could be useful for direct antifungal susceptibility testing on blood samples and basic research studies (e.g., the evaluation of the in vitro activity of antifungal combinations). This review summarizes the available data on the performance and potential use of the gradient strip method.https://www.mdpi.com/2309-608X/5/4/108gradient strip methodetestantifungal susceptibility testing |
| spellingShingle | Eric Dannaoui Ana Espinel-Ingroff Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review Journal of Fungi gradient strip method etest antifungal susceptibility testing |
| title | Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review |
| title_full | Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review |
| title_fullStr | Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review |
| title_full_unstemmed | Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review |
| title_short | Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review |
| title_sort | antifungal susceptibly testing by concentration gradient strip etest method for fungal isolates a review |
| topic | gradient strip method etest antifungal susceptibility testing |
| url | https://www.mdpi.com/2309-608X/5/4/108 |
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