Construction of a Ginseng Root-Meristem Sensor and a Sensing Kinetics Study on the Main Nitrogen Nutrients
Severe continuous cropping obstacles exist in ginseng cultivation. In order to assess these obstacles, a “sandwich” ginseng root tissue sensor was developed for the kinetic determination of five nitrogen nutrients. The results showed that the sensing parameters of the sensor reached an ultrasensitiv...
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/1424-8220/21/3/681 |
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| author | Shiang Wang Dingqiang Lu Guangchang Pang |
| author_facet | Shiang Wang Dingqiang Lu Guangchang Pang |
| author_sort | Shiang Wang |
| collection | DOAJ |
| description | Severe continuous cropping obstacles exist in ginseng cultivation. In order to assess these obstacles, a “sandwich” ginseng root tissue sensor was developed for the kinetic determination of five nitrogen nutrients. The results showed that the sensing parameters of the sensor reached an ultrasensitive level (limit of detection up to 5.451 × 10<sup>−24</sup> mol/L) for the five nitrogen nutrients, and exhibited good stability and reproducibility. In the order of two-, four-, and six-year-old ginseng plants, the sensitivity to inorganic nitrogen nutrients (sodium nitrate and urea) showed an upward trend following an initial decline (the interconnected allosteric constant Ka values acted as the parameter). The fluctuations in sensor sensitivity to organic nitrogen nutrients, specifically nucleotides (disodium inosinate and disodium guanylate), were relatively small. The sensor sensitivity of two-, four-, and six-year-old ginseng plants to sodium glutamate was 9.277 × 10<sup>−19</sup> mol/L, 6.980 × 10<sup>−21</sup> mol/L, and 5.451 × 10<sup>−24</sup> mol/L, respectively. Based on the survival rate of the seedlings and mortality rate of the ginseng in each age group, a Hardy–Weinberg equilibrium analysis was carried out. The results showed that the sensing ability of the root system to sodium glutamate may be an important factor affecting its survival under continuous cropping obstacles with increasing age. |
| first_indexed | 2024-03-09T04:12:34Z |
| format | Article |
| id | doaj.art-3f3500066e5e41b082fd7674e5da5733 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T04:12:34Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-3f3500066e5e41b082fd7674e5da57332023-12-03T13:58:19ZengMDPI AGSensors1424-82202021-01-0121368110.3390/s21030681Construction of a Ginseng Root-Meristem Sensor and a Sensing Kinetics Study on the Main Nitrogen NutrientsShiang Wang0Dingqiang Lu1Guangchang Pang2Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300314, ChinaTianjin Key Laboratory of Food Biotechnology, College of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300314, ChinaTianjin Key Laboratory of Food Biotechnology, College of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300314, ChinaSevere continuous cropping obstacles exist in ginseng cultivation. In order to assess these obstacles, a “sandwich” ginseng root tissue sensor was developed for the kinetic determination of five nitrogen nutrients. The results showed that the sensing parameters of the sensor reached an ultrasensitive level (limit of detection up to 5.451 × 10<sup>−24</sup> mol/L) for the five nitrogen nutrients, and exhibited good stability and reproducibility. In the order of two-, four-, and six-year-old ginseng plants, the sensitivity to inorganic nitrogen nutrients (sodium nitrate and urea) showed an upward trend following an initial decline (the interconnected allosteric constant Ka values acted as the parameter). The fluctuations in sensor sensitivity to organic nitrogen nutrients, specifically nucleotides (disodium inosinate and disodium guanylate), were relatively small. The sensor sensitivity of two-, four-, and six-year-old ginseng plants to sodium glutamate was 9.277 × 10<sup>−19</sup> mol/L, 6.980 × 10<sup>−21</sup> mol/L, and 5.451 × 10<sup>−24</sup> mol/L, respectively. Based on the survival rate of the seedlings and mortality rate of the ginseng in each age group, a Hardy–Weinberg equilibrium analysis was carried out. The results showed that the sensing ability of the root system to sodium glutamate may be an important factor affecting its survival under continuous cropping obstacles with increasing age.https://www.mdpi.com/1424-8220/21/3/681ginsengnitrogen nutrientscontinuous croppingroot meristemelectrochemical sensor |
| spellingShingle | Shiang Wang Dingqiang Lu Guangchang Pang Construction of a Ginseng Root-Meristem Sensor and a Sensing Kinetics Study on the Main Nitrogen Nutrients Sensors ginseng nitrogen nutrients continuous cropping root meristem electrochemical sensor |
| title | Construction of a Ginseng Root-Meristem Sensor and a Sensing Kinetics Study on the Main Nitrogen Nutrients |
| title_full | Construction of a Ginseng Root-Meristem Sensor and a Sensing Kinetics Study on the Main Nitrogen Nutrients |
| title_fullStr | Construction of a Ginseng Root-Meristem Sensor and a Sensing Kinetics Study on the Main Nitrogen Nutrients |
| title_full_unstemmed | Construction of a Ginseng Root-Meristem Sensor and a Sensing Kinetics Study on the Main Nitrogen Nutrients |
| title_short | Construction of a Ginseng Root-Meristem Sensor and a Sensing Kinetics Study on the Main Nitrogen Nutrients |
| title_sort | construction of a ginseng root meristem sensor and a sensing kinetics study on the main nitrogen nutrients |
| topic | ginseng nitrogen nutrients continuous cropping root meristem electrochemical sensor |
| url | https://www.mdpi.com/1424-8220/21/3/681 |
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