Effect of oral moisturizers containing chitosan and poloxamer 407 on biofilm formation of Candida species and Streptococcus mutans: in vitro
Oral moisturizers are topical agents which can directly relieve xerostomia and hyposalivation. This study evaluated the effects of three newly developed oral moisturizers (1) a formulation containing 0.5% chitosan (CHI) with 15% poloxamer 407 (P407), (2) a formulation containing only 0.5% CHI, and...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2022-10-01
|
| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://sjst.psu.ac.th/journal/44-5/19.pdf |
| _version_ | 1797839721033891840 |
|---|---|
| author | Wilairat Sarideechaigul Suwipa Ungphaiboon Hathaipan Ardpolthai Arnanee Jehsama-ae Kulrudee Sangsuttiwongsa Suwimol Taweechaisupapong |
| author_facet | Wilairat Sarideechaigul Suwipa Ungphaiboon Hathaipan Ardpolthai Arnanee Jehsama-ae Kulrudee Sangsuttiwongsa Suwimol Taweechaisupapong |
| author_sort | Wilairat Sarideechaigul |
| collection | DOAJ |
| description | Oral moisturizers are topical agents which can directly relieve xerostomia and hyposalivation. This study evaluated the
effects of three newly developed oral moisturizers (1) a formulation containing 0.5% chitosan (CHI) with 15% poloxamer 407
(P407), (2) a formulation containing only 0.5% CHI, and (3) a formulation containing only 15% P407, on adhesion and biofilm
formation of three Candida strains, i.e., Candida albicans DMST 5815, Candida krusei and Candida tropicalis from clinical
isolates and Streptococcous mutans DMST 18777, compared with a commercially available oral moisturizer by using crystal
violet assay. The results showed, in contrast to the commercial oral moisturizer, a significant reduction of Candida species and S.
mutans adhesion and biofilm formation after exposure to the three formulations (P < 0.05). The formulation containing CHI with
P407 exhibited the strongest anti-adhesion and anti-biofilm properties against Candida species. At the same time, these
combinations of CHI with P407 formulation may not be good for against S. mutans adhesion and biofilm. In contrast, the
formulation containing only CHI displayed strikingly stronger anti-adhesion and anti-biofilm effects against S. mutans than the
CHI with P407 formulation. This evidence confirms the great potential of anti-adhesion and anti-biofilm properties of CHI
against Candida species and S. mutans. |
| first_indexed | 2024-04-09T16:02:43Z |
| format | Article |
| id | doaj.art-3f35a840a07f43d38a1728f833bcc7e8 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-09T16:02:43Z |
| publishDate | 2022-10-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-3f35a840a07f43d38a1728f833bcc7e82023-04-25T09:29:37ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952022-10-014451298130510.14456/sjst-psu.2022.169Effect of oral moisturizers containing chitosan and poloxamer 407 on biofilm formation of Candida species and Streptococcus mutans: in vitroWilairat Sarideechaigul0Suwipa Ungphaiboon1Hathaipan Ardpolthai2Arnanee Jehsama-ae3Kulrudee Sangsuttiwongsa4Suwimol Taweechaisupapong5Department of Oral Biomedical Science, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandDrug Delivery System Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90110 ThailandDepartment of Oral Biomedical Science, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandDepartment of Oral Biomedical Science, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandDepartment of Oral Biomedical Science, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandDepartment of Oral Biomedical Science, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandOral moisturizers are topical agents which can directly relieve xerostomia and hyposalivation. This study evaluated the effects of three newly developed oral moisturizers (1) a formulation containing 0.5% chitosan (CHI) with 15% poloxamer 407 (P407), (2) a formulation containing only 0.5% CHI, and (3) a formulation containing only 15% P407, on adhesion and biofilm formation of three Candida strains, i.e., Candida albicans DMST 5815, Candida krusei and Candida tropicalis from clinical isolates and Streptococcous mutans DMST 18777, compared with a commercially available oral moisturizer by using crystal violet assay. The results showed, in contrast to the commercial oral moisturizer, a significant reduction of Candida species and S. mutans adhesion and biofilm formation after exposure to the three formulations (P < 0.05). The formulation containing CHI with P407 exhibited the strongest anti-adhesion and anti-biofilm properties against Candida species. At the same time, these combinations of CHI with P407 formulation may not be good for against S. mutans adhesion and biofilm. In contrast, the formulation containing only CHI displayed strikingly stronger anti-adhesion and anti-biofilm effects against S. mutans than the CHI with P407 formulation. This evidence confirms the great potential of anti-adhesion and anti-biofilm properties of CHI against Candida species and S. mutans.https://sjst.psu.ac.th/journal/44-5/19.pdforal moisturizerchitosanpoloxamer 407candida speciesstreptococcus mutans |
| spellingShingle | Wilairat Sarideechaigul Suwipa Ungphaiboon Hathaipan Ardpolthai Arnanee Jehsama-ae Kulrudee Sangsuttiwongsa Suwimol Taweechaisupapong Effect of oral moisturizers containing chitosan and poloxamer 407 on biofilm formation of Candida species and Streptococcus mutans: in vitro Songklanakarin Journal of Science and Technology (SJST) oral moisturizer chitosan poloxamer 407 candida species streptococcus mutans |
| title | Effect of oral moisturizers containing chitosan and poloxamer 407 on biofilm formation of Candida species and Streptococcus mutans: in vitro |
| title_full | Effect of oral moisturizers containing chitosan and poloxamer 407 on biofilm formation of Candida species and Streptococcus mutans: in vitro |
| title_fullStr | Effect of oral moisturizers containing chitosan and poloxamer 407 on biofilm formation of Candida species and Streptococcus mutans: in vitro |
| title_full_unstemmed | Effect of oral moisturizers containing chitosan and poloxamer 407 on biofilm formation of Candida species and Streptococcus mutans: in vitro |
| title_short | Effect of oral moisturizers containing chitosan and poloxamer 407 on biofilm formation of Candida species and Streptococcus mutans: in vitro |
| title_sort | effect of oral moisturizers containing chitosan and poloxamer 407 on biofilm formation of candida species and streptococcus mutans in vitro |
| topic | oral moisturizer chitosan poloxamer 407 candida species streptococcus mutans |
| url | https://sjst.psu.ac.th/journal/44-5/19.pdf |
| work_keys_str_mv | AT wilairatsarideechaigul effectoforalmoisturizerscontainingchitosanandpoloxamer407onbiofilmformationofcandidaspeciesandstreptococcusmutansinvitro AT suwipaungphaiboon effectoforalmoisturizerscontainingchitosanandpoloxamer407onbiofilmformationofcandidaspeciesandstreptococcusmutansinvitro AT hathaipanardpolthai effectoforalmoisturizerscontainingchitosanandpoloxamer407onbiofilmformationofcandidaspeciesandstreptococcusmutansinvitro AT arnaneejehsamaae effectoforalmoisturizerscontainingchitosanandpoloxamer407onbiofilmformationofcandidaspeciesandstreptococcusmutansinvitro AT kulrudeesangsuttiwongsa effectoforalmoisturizerscontainingchitosanandpoloxamer407onbiofilmformationofcandidaspeciesandstreptococcusmutansinvitro AT suwimoltaweechaisupapong effectoforalmoisturizerscontainingchitosanandpoloxamer407onbiofilmformationofcandidaspeciesandstreptococcusmutansinvitro |