Rapid and ultrasensitive detection of SARS-CoV-2 spike protein based on upconversion luminescence biosensor for COVID-19 point-of-care diagnostics
Here, we firstly introduce a detection system consisting of upconversion nanoparticles (UCNPs) and Au nanorods (AuNRs) for an ultrasensitive, rapid, quantitative and on-site detection of SARS-CoV-2 spike (S) protein based on Förster resonance energy transfer (FRET) effect. Briefly, the UCNPs capture...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522008851 |
| _version_ | 1797990291491258368 |
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| author | Lihua Li Menglin Song Xinyue Lao Sin-Yi Pang Yuan Liu Man-Chung Wong Yingjin Ma Mo Yang Jianhua Hao |
| author_facet | Lihua Li Menglin Song Xinyue Lao Sin-Yi Pang Yuan Liu Man-Chung Wong Yingjin Ma Mo Yang Jianhua Hao |
| author_sort | Lihua Li |
| collection | DOAJ |
| description | Here, we firstly introduce a detection system consisting of upconversion nanoparticles (UCNPs) and Au nanorods (AuNRs) for an ultrasensitive, rapid, quantitative and on-site detection of SARS-CoV-2 spike (S) protein based on Förster resonance energy transfer (FRET) effect. Briefly, the UCNPs capture the S protein of lysed SARS-CoV-2 in the swabs and subsequently they are bound with the anti-S antibodies modified AuNRs, resulting in significant nonradiative transitions from UCNPs (donors) to AuNRs (acceptors) at 480 nm and 800 nm, respectively. Notably, the specific recognition and quantitation of S protein can be realized in minutes at 800 nm because of the low autofluorescence and high Yb-Tm energy transfer in upconversion process. Inspiringly, the limit of detection (LOD) of the S protein can reach down to 1.06 fg mL−1, while the recognition of nucleocapsid protein is also comparable with a commercial test kit in a shorter time (only 5 min). The established strategy is technically superior to those reported point-of-care biosensors in terms of detection time, cost, and sensitivity, which paves a new avenue for future on-site rapid viral screening and point-of-care diagnostics. |
| first_indexed | 2024-04-11T08:34:06Z |
| format | Article |
| id | doaj.art-3f36dba6341a450c89c9048d6d444903 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-11T08:34:06Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-3f36dba6341a450c89c9048d6d4449032022-12-22T04:34:23ZengElsevierMaterials & Design0264-12752022-11-01223111263Rapid and ultrasensitive detection of SARS-CoV-2 spike protein based on upconversion luminescence biosensor for COVID-19 point-of-care diagnosticsLihua Li0Menglin Song1Xinyue Lao2Sin-Yi Pang3Yuan Liu4Man-Chung Wong5Yingjin Ma6Mo Yang7Jianhua Hao8Department of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China; Corresponding author.Here, we firstly introduce a detection system consisting of upconversion nanoparticles (UCNPs) and Au nanorods (AuNRs) for an ultrasensitive, rapid, quantitative and on-site detection of SARS-CoV-2 spike (S) protein based on Förster resonance energy transfer (FRET) effect. Briefly, the UCNPs capture the S protein of lysed SARS-CoV-2 in the swabs and subsequently they are bound with the anti-S antibodies modified AuNRs, resulting in significant nonradiative transitions from UCNPs (donors) to AuNRs (acceptors) at 480 nm and 800 nm, respectively. Notably, the specific recognition and quantitation of S protein can be realized in minutes at 800 nm because of the low autofluorescence and high Yb-Tm energy transfer in upconversion process. Inspiringly, the limit of detection (LOD) of the S protein can reach down to 1.06 fg mL−1, while the recognition of nucleocapsid protein is also comparable with a commercial test kit in a shorter time (only 5 min). The established strategy is technically superior to those reported point-of-care biosensors in terms of detection time, cost, and sensitivity, which paves a new avenue for future on-site rapid viral screening and point-of-care diagnostics.http://www.sciencedirect.com/science/article/pii/S0264127522008851Upconversion nanoparticlesAu nanorodsCOVID-19 point-of-care diagnosticsFRET effectSARS-CoV-2 spike protein |
| spellingShingle | Lihua Li Menglin Song Xinyue Lao Sin-Yi Pang Yuan Liu Man-Chung Wong Yingjin Ma Mo Yang Jianhua Hao Rapid and ultrasensitive detection of SARS-CoV-2 spike protein based on upconversion luminescence biosensor for COVID-19 point-of-care diagnostics Materials & Design Upconversion nanoparticles Au nanorods COVID-19 point-of-care diagnostics FRET effect SARS-CoV-2 spike protein |
| title | Rapid and ultrasensitive detection of SARS-CoV-2 spike protein based on upconversion luminescence biosensor for COVID-19 point-of-care diagnostics |
| title_full | Rapid and ultrasensitive detection of SARS-CoV-2 spike protein based on upconversion luminescence biosensor for COVID-19 point-of-care diagnostics |
| title_fullStr | Rapid and ultrasensitive detection of SARS-CoV-2 spike protein based on upconversion luminescence biosensor for COVID-19 point-of-care diagnostics |
| title_full_unstemmed | Rapid and ultrasensitive detection of SARS-CoV-2 spike protein based on upconversion luminescence biosensor for COVID-19 point-of-care diagnostics |
| title_short | Rapid and ultrasensitive detection of SARS-CoV-2 spike protein based on upconversion luminescence biosensor for COVID-19 point-of-care diagnostics |
| title_sort | rapid and ultrasensitive detection of sars cov 2 spike protein based on upconversion luminescence biosensor for covid 19 point of care diagnostics |
| topic | Upconversion nanoparticles Au nanorods COVID-19 point-of-care diagnostics FRET effect SARS-CoV-2 spike protein |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522008851 |
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