pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH.

pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH.

We report the development of a genetically encodable and ratiometic pH probe named "pHlash" that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation. The pHlash sensor-composed of a donor luciferase that is genetically fused to a Venus fluorophore-...

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Bibliographic Details
Main Authors: Yunfei Zhang, Qiguang Xie, J Brian Robertson, Carl Hirschie Johnson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22905204/?tool=EBI

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