Form, synapses and orientation topography of a new cell type in layer 6 of the cat’s primary visual cortex
Abstract Here we report the morpho-functional features of a novel type of deep-layer neuron. The neuron was selected from a large pool of intracellularly labelled cells based on the large cell body, numerous spine-free dendrites with an overall interneuron morphology. However, the axon gave off long...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19746-9 |
| _version_ | 1797995499858427904 |
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| author | Mohit Srivastava Cintia Angel Réka Eszter Kisvárday Zsolt Kocsis András Stelescu Petra Talapka Zoltán Kisvárday |
| author_facet | Mohit Srivastava Cintia Angel Réka Eszter Kisvárday Zsolt Kocsis András Stelescu Petra Talapka Zoltán Kisvárday |
| author_sort | Mohit Srivastava |
| collection | DOAJ |
| description | Abstract Here we report the morpho-functional features of a novel type of deep-layer neuron. The neuron was selected from a large pool of intracellularly labelled cells based on the large cell body, numerous spine-free dendrites with an overall interneuron morphology. However, the axon gave off long-range axons up to 2.8 mm from the parent soma in layers 5/6 before entering the white matter. The boutons were uniformly distributed along the axon without forming distinct clusters. Dendritic length, surface area and volume values were at least 3 times larger than any known cortical neuron types with the exception of giant pyramidal cells of layer 5. Electron microscopy of the boutons revealed that they targeted dendritic spines (78%) and less frequently dendritic shafts (22%). Nearly half of the postsynaptic dendrites were immunopositive to GABA. Superimposing the axonal field on the orientation map obtained with optical imaging showed a preponderance of boutons to cross-orientations (38%) and an equal representation of iso- and oblique orientations (31%). The results suggest an integrating role for the layer 6 stellate neuron which projects to a functionally broad range of neurons in the deep cortical layers and to other cortical and/or subcortical regions. |
| first_indexed | 2024-04-11T10:01:39Z |
| format | Article |
| id | doaj.art-3f3726a1853b48548316a7f5522d9499 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T10:01:39Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3f3726a1853b48548316a7f5522d94992022-12-22T04:30:22ZengNature PortfolioScientific Reports2045-23222022-09-0112111510.1038/s41598-022-19746-9Form, synapses and orientation topography of a new cell type in layer 6 of the cat’s primary visual cortexMohit Srivastava0Cintia Angel1Réka Eszter Kisvárday2Zsolt Kocsis3András Stelescu4Petra Talapka5Zoltán Kisvárday6ELKH-DE Neuroscience Research Group, Department of Anatomy, Histology and Embryology, University of DebrecenELKH-DE Neuroscience Research Group, Department of Anatomy, Histology and Embryology, University of DebrecenDepartment of Neuroanatomy and Molecular Brain Research, Faculty of Medicine, Ruhr-Universitat BochumDepartment of Anatomy, Histology and Embryology, Faculty of Medicine, University of DebrecenDepartment of Anatomy, Histology and Embryology, Faculty of Medicine, University of DebrecenELKH-DE Neuroscience Research Group, Department of Anatomy, Histology and Embryology, University of DebrecenELKH-DE Neuroscience Research Group, Department of Anatomy, Histology and Embryology, University of DebrecenAbstract Here we report the morpho-functional features of a novel type of deep-layer neuron. The neuron was selected from a large pool of intracellularly labelled cells based on the large cell body, numerous spine-free dendrites with an overall interneuron morphology. However, the axon gave off long-range axons up to 2.8 mm from the parent soma in layers 5/6 before entering the white matter. The boutons were uniformly distributed along the axon without forming distinct clusters. Dendritic length, surface area and volume values were at least 3 times larger than any known cortical neuron types with the exception of giant pyramidal cells of layer 5. Electron microscopy of the boutons revealed that they targeted dendritic spines (78%) and less frequently dendritic shafts (22%). Nearly half of the postsynaptic dendrites were immunopositive to GABA. Superimposing the axonal field on the orientation map obtained with optical imaging showed a preponderance of boutons to cross-orientations (38%) and an equal representation of iso- and oblique orientations (31%). The results suggest an integrating role for the layer 6 stellate neuron which projects to a functionally broad range of neurons in the deep cortical layers and to other cortical and/or subcortical regions.https://doi.org/10.1038/s41598-022-19746-9 |
| spellingShingle | Mohit Srivastava Cintia Angel Réka Eszter Kisvárday Zsolt Kocsis András Stelescu Petra Talapka Zoltán Kisvárday Form, synapses and orientation topography of a new cell type in layer 6 of the cat’s primary visual cortex Scientific Reports |
| title | Form, synapses and orientation topography of a new cell type in layer 6 of the cat’s primary visual cortex |
| title_full | Form, synapses and orientation topography of a new cell type in layer 6 of the cat’s primary visual cortex |
| title_fullStr | Form, synapses and orientation topography of a new cell type in layer 6 of the cat’s primary visual cortex |
| title_full_unstemmed | Form, synapses and orientation topography of a new cell type in layer 6 of the cat’s primary visual cortex |
| title_short | Form, synapses and orientation topography of a new cell type in layer 6 of the cat’s primary visual cortex |
| title_sort | form synapses and orientation topography of a new cell type in layer 6 of the cat s primary visual cortex |
| url | https://doi.org/10.1038/s41598-022-19746-9 |
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