Chemical Composition, Antioxidant and Hypoglycemic Activities of Phenolic Fractions from Sargassum pallidum
Three forms of soluble phenolics, free phenolics (FP), esterified-bound phenolics (EP) and glycoside-bound phenolics (GP), were extracted from Sargassum pallidum, and their chemical composition was identified by ultra-high performance liquid chromatography-quadrupole-time of flight-tandem mass spect...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-05-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-10-035.pdf |
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| author | XIE Xing, WANG Siyu, WANG Peixin, ZHANG Lu, TU Zongcai, CHEN Chun, FU Xiong |
| author_facet | XIE Xing, WANG Siyu, WANG Peixin, ZHANG Lu, TU Zongcai, CHEN Chun, FU Xiong |
| author_sort | XIE Xing, WANG Siyu, WANG Peixin, ZHANG Lu, TU Zongcai, CHEN Chun, FU Xiong |
| collection | DOAJ |
| description | Three forms of soluble phenolics, free phenolics (FP), esterified-bound phenolics (EP) and glycoside-bound phenolics (GP), were extracted from Sargassum pallidum, and their chemical composition was identified by ultra-high performance liquid chromatography-quadrupole-time of flight-tandem mass spectrometry (UPLC-QTOF-MS/MS). Their antioxidant and α-glucosidase inhibitory activities in vitro were evaluated. Results demonstrated that the soluble phenolics in S. pallidum mainly existed in a bound form, GP and EP fractions contained the highest contents of total phenolics (68.30 mg/g) and flavonoids (83.49 mg/g), respectively. A total of 22 compounds were identified in the three phenolic fractions, including three simple phenolics, four flavonoids and two phenolic acids. Biological activity tests indicated GP had the highest cellular antioxidant activity (CAA value = 18.18 μmol/g), 1,1-dipheny1-2-picrylhydrazyl (DPPH) radical scavenging activity (IC50 = 84.6 μg/mL) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity (IC50 = 127.01 μg/mL), while EP had the strongest oxygen radical absorbance capacity (5 322.85 μmol/g) and α-glucosidase inhibitory activity with IC50 value of 27.09 μg/mL. The hypoglycemic activity of EP was significantly higher than that of acarbose. Inhibition kinetics analysis showed that the inhibition mode of α-glucosidase by the three phenolic fractions was a mixed type. In conclusion, bound phenolics from Sargassum pallidum have excellent antioxidant and α-glucosidase inhibitory activities, which will provide a scientific basis for the development of natural antioxidants and hypoglycemic foods. |
| first_indexed | 2024-03-13T05:53:40Z |
| format | Article |
| id | doaj.art-3f3b720e122f42cabf29e205035e92a8 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-13T05:53:40Z |
| publishDate | 2023-05-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj.art-3f3b720e122f42cabf29e205035e92a82023-06-13T07:42:12ZengChina Food Publishing CompanyShipin Kexue1002-66302023-05-01441028129010.7506/spkx1002-6630-20220801-006Chemical Composition, Antioxidant and Hypoglycemic Activities of Phenolic Fractions from Sargassum pallidumXIE Xing, WANG Siyu, WANG Peixin, ZHANG Lu, TU Zongcai, CHEN Chun, FU Xiong0(1. School of Health, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China;2. School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China)Three forms of soluble phenolics, free phenolics (FP), esterified-bound phenolics (EP) and glycoside-bound phenolics (GP), were extracted from Sargassum pallidum, and their chemical composition was identified by ultra-high performance liquid chromatography-quadrupole-time of flight-tandem mass spectrometry (UPLC-QTOF-MS/MS). Their antioxidant and α-glucosidase inhibitory activities in vitro were evaluated. Results demonstrated that the soluble phenolics in S. pallidum mainly existed in a bound form, GP and EP fractions contained the highest contents of total phenolics (68.30 mg/g) and flavonoids (83.49 mg/g), respectively. A total of 22 compounds were identified in the three phenolic fractions, including three simple phenolics, four flavonoids and two phenolic acids. Biological activity tests indicated GP had the highest cellular antioxidant activity (CAA value = 18.18 μmol/g), 1,1-dipheny1-2-picrylhydrazyl (DPPH) radical scavenging activity (IC50 = 84.6 μg/mL) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity (IC50 = 127.01 μg/mL), while EP had the strongest oxygen radical absorbance capacity (5 322.85 μmol/g) and α-glucosidase inhibitory activity with IC50 value of 27.09 μg/mL. The hypoglycemic activity of EP was significantly higher than that of acarbose. Inhibition kinetics analysis showed that the inhibition mode of α-glucosidase by the three phenolic fractions was a mixed type. In conclusion, bound phenolics from Sargassum pallidum have excellent antioxidant and α-glucosidase inhibitory activities, which will provide a scientific basis for the development of natural antioxidants and hypoglycemic foods.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-10-035.pdfsargassum pallidum; phenolics; antioxidant; α-glucosidase inhibitory activity; ultra-high performance liquid chromatography-quadrupole-time of flight-tandem mass spectrometry |
| spellingShingle | XIE Xing, WANG Siyu, WANG Peixin, ZHANG Lu, TU Zongcai, CHEN Chun, FU Xiong Chemical Composition, Antioxidant and Hypoglycemic Activities of Phenolic Fractions from Sargassum pallidum Shipin Kexue sargassum pallidum; phenolics; antioxidant; α-glucosidase inhibitory activity; ultra-high performance liquid chromatography-quadrupole-time of flight-tandem mass spectrometry |
| title | Chemical Composition, Antioxidant and Hypoglycemic Activities of Phenolic Fractions from Sargassum pallidum |
| title_full | Chemical Composition, Antioxidant and Hypoglycemic Activities of Phenolic Fractions from Sargassum pallidum |
| title_fullStr | Chemical Composition, Antioxidant and Hypoglycemic Activities of Phenolic Fractions from Sargassum pallidum |
| title_full_unstemmed | Chemical Composition, Antioxidant and Hypoglycemic Activities of Phenolic Fractions from Sargassum pallidum |
| title_short | Chemical Composition, Antioxidant and Hypoglycemic Activities of Phenolic Fractions from Sargassum pallidum |
| title_sort | chemical composition antioxidant and hypoglycemic activities of phenolic fractions from sargassum pallidum |
| topic | sargassum pallidum; phenolics; antioxidant; α-glucosidase inhibitory activity; ultra-high performance liquid chromatography-quadrupole-time of flight-tandem mass spectrometry |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-10-035.pdf |
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