An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin

Abstract Background The dermal papilla cells are a specialized population of mesenchymal cells located at the base of the hair follicle (HF), which possess the capacity to regulate HF morphogenesis and regeneration. However, lack of cell-type specific surface markers restricts the isolation of DP ce...

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Main Authors: Lijuan Du, Yuyang Gan, Bowen Zheng, Junfei Huang, Zhiqi Hu, Yong Miao
Format: Article
Language:English
Published: BMC 2023-05-01
Series:Stem Cell Research & Therapy
Subjects:
Online Access:https://doi.org/10.1186/s13287-023-03343-2
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author Lijuan Du
Yuyang Gan
Bowen Zheng
Junfei Huang
Zhiqi Hu
Yong Miao
author_facet Lijuan Du
Yuyang Gan
Bowen Zheng
Junfei Huang
Zhiqi Hu
Yong Miao
author_sort Lijuan Du
collection DOAJ
description Abstract Background The dermal papilla cells are a specialized population of mesenchymal cells located at the base of the hair follicle (HF), which possess the capacity to regulate HF morphogenesis and regeneration. However, lack of cell-type specific surface markers restricts the isolation of DP cells and application for tissue engineering purposes. Methods We describe a novel force-triggered density gradient sedimentation (FDGS) method to efficiently obtain purified follicular DP-spheres cells from neonatal mouse back skin, utilizing only centrifugation and optimized density gradients. Results Expression of characteristic DP cell markers, alkaline phosphatase, β-catenin, versican, and neural cell adhesion molecules, were confirmed by immunofluorescence. Further, the patch assays demonstrated that DP cells maintained their hair regenerative capacity in vivo. Compared with current methods, including microdissection and fluorescence-activated cell sorting, the FDGS technique is simpler and more efficient for isolating DP cells from neonatal mouse skin. Conclusions The FDGS method will improve the research potential of neonatal mouse pelage-derived DP cells for tissue engineering purposes.
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spelling doaj.art-3f3dcdc05c9a48039302e0e1894cf2d82023-05-28T11:10:02ZengBMCStem Cell Research & Therapy1757-65122023-05-0114111010.1186/s13287-023-03343-2An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skinLijuan Du0Yuyang Gan1Bowen Zheng2Junfei Huang3Zhiqi Hu4Yong Miao5Department of Plastic and Aesthetic Surgery, Nan Fang Hospital of Southern Medical UniversityDepartment of Plastic and Aesthetic Surgery, Nan Fang Hospital of Southern Medical UniversityDepartment of Plastic and Aesthetic Surgery, Nan Fang Hospital of Southern Medical UniversityDepartment of Plastic and Aesthetic Surgery, Nan Fang Hospital of Southern Medical UniversityDepartment of Plastic and Aesthetic Surgery, Nan Fang Hospital of Southern Medical UniversityDepartment of Plastic and Aesthetic Surgery, Nan Fang Hospital of Southern Medical UniversityAbstract Background The dermal papilla cells are a specialized population of mesenchymal cells located at the base of the hair follicle (HF), which possess the capacity to regulate HF morphogenesis and regeneration. However, lack of cell-type specific surface markers restricts the isolation of DP cells and application for tissue engineering purposes. Methods We describe a novel force-triggered density gradient sedimentation (FDGS) method to efficiently obtain purified follicular DP-spheres cells from neonatal mouse back skin, utilizing only centrifugation and optimized density gradients. Results Expression of characteristic DP cell markers, alkaline phosphatase, β-catenin, versican, and neural cell adhesion molecules, were confirmed by immunofluorescence. Further, the patch assays demonstrated that DP cells maintained their hair regenerative capacity in vivo. Compared with current methods, including microdissection and fluorescence-activated cell sorting, the FDGS technique is simpler and more efficient for isolating DP cells from neonatal mouse skin. Conclusions The FDGS method will improve the research potential of neonatal mouse pelage-derived DP cells for tissue engineering purposes.https://doi.org/10.1186/s13287-023-03343-2Pelage follicleDermal papillaForce-triggered density gradient sedimentationFDGS
spellingShingle Lijuan Du
Yuyang Gan
Bowen Zheng
Junfei Huang
Zhiqi Hu
Yong Miao
An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin
Stem Cell Research & Therapy
Pelage follicle
Dermal papilla
Force-triggered density gradient sedimentation
FDGS
title An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin
title_full An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin
title_fullStr An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin
title_full_unstemmed An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin
title_short An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin
title_sort optimized force triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin
topic Pelage follicle
Dermal papilla
Force-triggered density gradient sedimentation
FDGS
url https://doi.org/10.1186/s13287-023-03343-2
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