Serum metabolomic profiles in dogs with chronic enteropathy
Abstract Background Metabolic profiles differ between healthy humans and those with inflammatory bowel disease. Few studies have examined metabolic profiles in dogs with chronic enteropathy (CE). Hypothesis Serum metabolic profiles of dogs with CE are significantly different from those of healthy do...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-09-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16419 |
| _version_ | 1811258712902336512 |
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| author | Hannah K. Walker Alisdair M. Boag Claudia Ottka Hannes Lohi Ian Handel Adam G. Gow Richard J. Mellanby |
| author_facet | Hannah K. Walker Alisdair M. Boag Claudia Ottka Hannes Lohi Ian Handel Adam G. Gow Richard J. Mellanby |
| author_sort | Hannah K. Walker |
| collection | DOAJ |
| description | Abstract Background Metabolic profiles differ between healthy humans and those with inflammatory bowel disease. Few studies have examined metabolic profiles in dogs with chronic enteropathy (CE). Hypothesis Serum metabolic profiles of dogs with CE are significantly different from those of healthy dogs. Animals Fifty‐five dogs with CE and 204 healthy controls. Methods A cross‐sectional study. The serum concentrations of 99 metabolites measured using a canine‐specific proton nuclear magnetic resonance spectroscopy platform were studied. A 2‐sample unpaired t‐test was used to compare the 2 study samples. The threshold for significance was set at P < .05 with a Bonferroni correction for each metabolite group. Results Nineteen metabolites and 18 indices of lipoprotein composition were significantly different between the CE and healthy dogs. Four metabolites were significantly higher in dogs with CE, including phenylalanine (mean and SD) (healthy: 0.0417 mmol/L; [SD] 0.0100; CE: 0.0480 mmol/L; SD: 0.0125; P value: <.001) and lactate (healthy: 1.8751 mmol/L; SD: 0.7808; CE: 2.4827 mmol/L; SD CE: 1.4166; P value: .003). Fifteen metabolites were significantly lower in dogs with CE, including total fatty acids, and glycine (healthy: 0.2273 mmol/L; SD: 0.0794; CE: 0.1828 mmol/L; SD CE: 0.0517; P value: <.001). Conclusions and Clinical Importance The metabolic profile of dogs with CE is significantly different from that of healthy dogs, this opens novel research avenues to develop better diagnostic and prognostic approaches as well as therapeutic trials. |
| first_indexed | 2024-04-12T18:18:43Z |
| format | Article |
| id | doaj.art-3f4297c6338541579f54e52002a537f6 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-12T18:18:43Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-3f4297c6338541579f54e52002a537f62022-12-22T03:21:32ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762022-09-013651752175910.1111/jvim.16419Serum metabolomic profiles in dogs with chronic enteropathyHannah K. Walker0Alisdair M. Boag1Claudia Ottka2Hannes Lohi3Ian Handel4Adam G. Gow5Richard J. Mellanby6The Royal (Dick) School of Veterinary Studies and the Roslin Institute, Easter Bush Campus The University of Edinburgh Midlothian United KingdomThe Royal (Dick) School of Veterinary Studies and the Roslin Institute, Easter Bush Campus The University of Edinburgh Midlothian United KingdomPetBiomics Ltd Helsinki FinlandPetBiomics Ltd Helsinki FinlandThe Royal (Dick) School of Veterinary Studies and the Roslin Institute, Easter Bush Campus The University of Edinburgh Midlothian United KingdomThe Royal (Dick) School of Veterinary Studies and the Roslin Institute, Easter Bush Campus The University of Edinburgh Midlothian United KingdomThe Royal (Dick) School of Veterinary Studies and the Roslin Institute, Easter Bush Campus The University of Edinburgh Midlothian United KingdomAbstract Background Metabolic profiles differ between healthy humans and those with inflammatory bowel disease. Few studies have examined metabolic profiles in dogs with chronic enteropathy (CE). Hypothesis Serum metabolic profiles of dogs with CE are significantly different from those of healthy dogs. Animals Fifty‐five dogs with CE and 204 healthy controls. Methods A cross‐sectional study. The serum concentrations of 99 metabolites measured using a canine‐specific proton nuclear magnetic resonance spectroscopy platform were studied. A 2‐sample unpaired t‐test was used to compare the 2 study samples. The threshold for significance was set at P < .05 with a Bonferroni correction for each metabolite group. Results Nineteen metabolites and 18 indices of lipoprotein composition were significantly different between the CE and healthy dogs. Four metabolites were significantly higher in dogs with CE, including phenylalanine (mean and SD) (healthy: 0.0417 mmol/L; [SD] 0.0100; CE: 0.0480 mmol/L; SD: 0.0125; P value: <.001) and lactate (healthy: 1.8751 mmol/L; SD: 0.7808; CE: 2.4827 mmol/L; SD CE: 1.4166; P value: .003). Fifteen metabolites were significantly lower in dogs with CE, including total fatty acids, and glycine (healthy: 0.2273 mmol/L; SD: 0.0794; CE: 0.1828 mmol/L; SD CE: 0.0517; P value: <.001). Conclusions and Clinical Importance The metabolic profile of dogs with CE is significantly different from that of healthy dogs, this opens novel research avenues to develop better diagnostic and prognostic approaches as well as therapeutic trials.https://doi.org/10.1111/jvim.16419amino acidcanineglycoprotein acetylslipidmetabolomics |
| spellingShingle | Hannah K. Walker Alisdair M. Boag Claudia Ottka Hannes Lohi Ian Handel Adam G. Gow Richard J. Mellanby Serum metabolomic profiles in dogs with chronic enteropathy Journal of Veterinary Internal Medicine amino acid canine glycoprotein acetyls lipid metabolomics |
| title | Serum metabolomic profiles in dogs with chronic enteropathy |
| title_full | Serum metabolomic profiles in dogs with chronic enteropathy |
| title_fullStr | Serum metabolomic profiles in dogs with chronic enteropathy |
| title_full_unstemmed | Serum metabolomic profiles in dogs with chronic enteropathy |
| title_short | Serum metabolomic profiles in dogs with chronic enteropathy |
| title_sort | serum metabolomic profiles in dogs with chronic enteropathy |
| topic | amino acid canine glycoprotein acetyls lipid metabolomics |
| url | https://doi.org/10.1111/jvim.16419 |
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