Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps;">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance
Hydrogen sulfide (H<sub>2</sub>S) is an important signaling molecule that regulates diverse cellular signaling pathways through persulfidation. Our previous study revealed that H<sub>2</sub>S is involved in the improvement of rice drought tolerance. However, the corresponding...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12119 |
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| author | Heng Zhou Ying Zhou Feng Zhang Wenxue Guan Ye Su Xingxing Yuan Yanjie Xie |
| author_facet | Heng Zhou Ying Zhou Feng Zhang Wenxue Guan Ye Su Xingxing Yuan Yanjie Xie |
| author_sort | Heng Zhou |
| collection | DOAJ |
| description | Hydrogen sulfide (H<sub>2</sub>S) is an important signaling molecule that regulates diverse cellular signaling pathways through persulfidation. Our previous study revealed that H<sub>2</sub>S is involved in the improvement of rice drought tolerance. However, the corresponding enzymatic sources of H<sub>2</sub>S and its regulatory mechanism in response to drought stress are not clear. Here, we cloned and characterized a putative <i><span style="font-variant: small-caps;">l</span>-cysteine desulfhydrase</i> (<i>LCD</i>) gene in rice, which encodes a protein possessing H<sub>2</sub>S-producing activity and was named <i>OsLCD1</i>. Overexpression of <i>OsLCD1</i> results in enhanced H<sub>2</sub>S production, persulfidation of total soluble protein, and confers rice drought tolerance. Further, we found that nitrate reductase (NR) activity was decreased under drought stress, and the inhibition of NR activity was controlled by endogenous H<sub>2</sub>S production. Persulfidation of NIA2, an NR isoform responsible for the main NR activity, led to a decrease in total NR activity in rice. Furthermore, drought stress-triggered inhibition of NR activity and persulfidation of NIA2 was intensified in the <i>OsLCD1</i> overexpression line. Phenotypical and molecular analysis revealed that mutation of <i>NIA2</i> enhanced rice drought tolerance by activating the expression of genes encoding antioxidant enzymes and ABA-responsive genes. Taken together, our results showed the role of <i>OsLCD1</i> in modulating H<sub>2</sub>S production and provided insight into H<sub>2</sub>S-regulated persulfidation of NIA2 in the control of rice drought stress. |
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| spelling | doaj.art-3f42c4ec2a9c4ca19abbe6186d9a10f52023-11-22T23:37:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221211910.3390/ijms222212119Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps;">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought ToleranceHeng Zhou0Ying Zhou1Feng Zhang2Wenxue Guan3Ye Su4Xingxing Yuan5Yanjie Xie6Laboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaLaboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaHydrogen sulfide (H<sub>2</sub>S) is an important signaling molecule that regulates diverse cellular signaling pathways through persulfidation. Our previous study revealed that H<sub>2</sub>S is involved in the improvement of rice drought tolerance. However, the corresponding enzymatic sources of H<sub>2</sub>S and its regulatory mechanism in response to drought stress are not clear. Here, we cloned and characterized a putative <i><span style="font-variant: small-caps;">l</span>-cysteine desulfhydrase</i> (<i>LCD</i>) gene in rice, which encodes a protein possessing H<sub>2</sub>S-producing activity and was named <i>OsLCD1</i>. Overexpression of <i>OsLCD1</i> results in enhanced H<sub>2</sub>S production, persulfidation of total soluble protein, and confers rice drought tolerance. Further, we found that nitrate reductase (NR) activity was decreased under drought stress, and the inhibition of NR activity was controlled by endogenous H<sub>2</sub>S production. Persulfidation of NIA2, an NR isoform responsible for the main NR activity, led to a decrease in total NR activity in rice. Furthermore, drought stress-triggered inhibition of NR activity and persulfidation of NIA2 was intensified in the <i>OsLCD1</i> overexpression line. Phenotypical and molecular analysis revealed that mutation of <i>NIA2</i> enhanced rice drought tolerance by activating the expression of genes encoding antioxidant enzymes and ABA-responsive genes. Taken together, our results showed the role of <i>OsLCD1</i> in modulating H<sub>2</sub>S production and provided insight into H<sub>2</sub>S-regulated persulfidation of NIA2 in the control of rice drought stress.https://www.mdpi.com/1422-0067/22/22/12119hydrogen sulfidepersulfidationdrought stressnitrate reductase<span style="font-variant: small-caps;">l</span>-cysteine desulfhydrase |
| spellingShingle | Heng Zhou Ying Zhou Feng Zhang Wenxue Guan Ye Su Xingxing Yuan Yanjie Xie Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps;">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance International Journal of Molecular Sciences hydrogen sulfide persulfidation drought stress nitrate reductase <span style="font-variant: small-caps;">l</span>-cysteine desulfhydrase |
| title | Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps;">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance |
| title_full | Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps;">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance |
| title_fullStr | Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps;">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance |
| title_full_unstemmed | Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps;">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance |
| title_short | Persulfidation of Nitrate Reductase 2 Is Involved in <span style="font-variant: small-caps;">l</span>-Cysteine Desulfhydrase-Regulated Rice Drought Tolerance |
| title_sort | persulfidation of nitrate reductase 2 is involved in span style font variant small caps l span cysteine desulfhydrase regulated rice drought tolerance |
| topic | hydrogen sulfide persulfidation drought stress nitrate reductase <span style="font-variant: small-caps;">l</span>-cysteine desulfhydrase |
| url | https://www.mdpi.com/1422-0067/22/22/12119 |
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