Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)
Kinnow mandarin (Citrus reticulata Blanco) is a popular citrus crop of northwestern India and it occupies maximum fruit area in Punjab. However, citrus juice processing industry is still suffering from delayed bitterness problem caused mainly by limonoid aglycones such as limonin. In order to study...
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University of Zagreb Faculty of Food Technology and Biotechnology
2018-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/299532 |
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| author | Sumedha Arora Prashant Mohanpuria Gurupkar Singh Sidhu Inderjit Singh Yadav Vandna Kumari |
| author_facet | Sumedha Arora Prashant Mohanpuria Gurupkar Singh Sidhu Inderjit Singh Yadav Vandna Kumari |
| author_sort | Sumedha Arora |
| collection | DOAJ |
| description | Kinnow mandarin (Citrus reticulata Blanco) is a popular citrus crop of northwestern India and it occupies maximum fruit area in Punjab. However, citrus juice processing industry is still suffering from delayed bitterness problem caused mainly by limonoid aglycones such as limonin. In order to study citrus limonoid metabolism, limonoid glucosyltransferase (LGT) gene, which encodes a natural debittering enzyme, was isolated from the fruit tissues of Kinnow mandarin. After confirmation and characterization, its full-length gene sequence (1533 bp) was submitted to National Centre for Biotechnology Information. Citrus reticulata limonoid glucosyltransferase (CrLGT) occupies a position on an independent branch in the largest subgroup and is phylogenetically different from those in other mandarin species like C. unshiu, showing its uniqueness in several features. The transcript expression of CrLGT, evaluated in different tissues such as young leaf, flavedo, albedo, sac covering and seed of Kinnow mandarin during early (90 days after flowering (DAF)), mid (150-210 DAF) and late (240 DAF) fruit developmental stages using semi-quantitative method, showed the highest expression in flavedo. Thus, it was concluded that the isolated LGT gene has an effect on limonoid metabolic engineering in citrus. Overexpression of this gene can reduce the delayed bitterness problem in citrus juice and enhance the accumulation of specific glucosides that have anticancer effects. |
| first_indexed | 2024-04-24T09:25:32Z |
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| id | doaj.art-3f444b39a5744a419ad4fdf82d59e37d |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:25:32Z |
| publishDate | 2018-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-3f444b39a5744a419ad4fdf82d59e37d2024-04-15T14:55:09ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062018-01-0156222823710.17113/ftb.56.02.18.5349Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco)Sumedha Arora0Prashant Mohanpuria1Gurupkar Singh Sidhu2Inderjit Singh Yadav3Vandna Kumari4School of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, IndiaSchool of Agricultural Biotechnology, Punjab Agricultural University, IN- 141004 Ludhiana, IndiaBotany Department, Punjabi University, IN-147002 Patiala, IndiaKinnow mandarin (Citrus reticulata Blanco) is a popular citrus crop of northwestern India and it occupies maximum fruit area in Punjab. However, citrus juice processing industry is still suffering from delayed bitterness problem caused mainly by limonoid aglycones such as limonin. In order to study citrus limonoid metabolism, limonoid glucosyltransferase (LGT) gene, which encodes a natural debittering enzyme, was isolated from the fruit tissues of Kinnow mandarin. After confirmation and characterization, its full-length gene sequence (1533 bp) was submitted to National Centre for Biotechnology Information. Citrus reticulata limonoid glucosyltransferase (CrLGT) occupies a position on an independent branch in the largest subgroup and is phylogenetically different from those in other mandarin species like C. unshiu, showing its uniqueness in several features. The transcript expression of CrLGT, evaluated in different tissues such as young leaf, flavedo, albedo, sac covering and seed of Kinnow mandarin during early (90 days after flowering (DAF)), mid (150-210 DAF) and late (240 DAF) fruit developmental stages using semi-quantitative method, showed the highest expression in flavedo. Thus, it was concluded that the isolated LGT gene has an effect on limonoid metabolic engineering in citrus. Overexpression of this gene can reduce the delayed bitterness problem in citrus juice and enhance the accumulation of specific glucosides that have anticancer effects.https://hrcak.srce.hr/file/299532Kinnow mandarindelayed bitternesslimonoid glucosyltransferaseanticancer propertiessemi-quantitative PCR |
| spellingShingle | Sumedha Arora Prashant Mohanpuria Gurupkar Singh Sidhu Inderjit Singh Yadav Vandna Kumari Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco) Food Technology and Biotechnology Kinnow mandarin delayed bitterness limonoid glucosyltransferase anticancer properties semi-quantitative PCR |
| title | Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco) |
| title_full | Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco) |
| title_fullStr | Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco) |
| title_full_unstemmed | Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco) |
| title_short | Cloning and Characterization of Limonoid Glucosyltransferase from Kinnow Mandarin (Citrus reticulata Blanco) |
| title_sort | cloning and characterization of limonoid glucosyltransferase from kinnow mandarin citrus reticulata blanco |
| topic | Kinnow mandarin delayed bitterness limonoid glucosyltransferase anticancer properties semi-quantitative PCR |
| url | https://hrcak.srce.hr/file/299532 |
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