Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E.
We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4321833?pdf=render |
| _version_ | 1819106489598476288 |
|---|---|
| author | Silvia Ratto-Kim Mark S de Souza Jeffrey R Currier Nicos Karasavvas John Sidney Morgane Rolland Anais Valencia-Micolta Sirinan Madnote Alessandro Sette Sorachai Nitayaphan Punnee Pitisuttuthum Jaranit Kaewkungwal Supachai Rerks-Ngarm Robert O'Connell Nelson Michael Merlin L Robb Mary Marovich Jerome H Kim |
| author_facet | Silvia Ratto-Kim Mark S de Souza Jeffrey R Currier Nicos Karasavvas John Sidney Morgane Rolland Anais Valencia-Micolta Sirinan Madnote Alessandro Sette Sorachai Nitayaphan Punnee Pitisuttuthum Jaranit Kaewkungwal Supachai Rerks-Ngarm Robert O'Connell Nelson Michael Merlin L Robb Mary Marovich Jerome H Kim |
| author_sort | Silvia Ratto-Kim |
| collection | DOAJ |
| description | We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody. |
| first_indexed | 2024-12-22T02:38:57Z |
| format | Article |
| id | doaj.art-3f48103a5af34f3c907fa9c1c7b22e76 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T02:38:57Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3f48103a5af34f3c907fa9c1c7b22e762022-12-21T18:41:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011558210.1371/journal.pone.0115582Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E.Silvia Ratto-KimMark S de SouzaJeffrey R CurrierNicos KarasavvasJohn SidneyMorgane RollandAnais Valencia-MicoltaSirinan MadnoteAlessandro SetteSorachai NitayaphanPunnee PitisuttuthumJaranit KaewkungwalSupachai Rerks-NgarmRobert O'ConnellNelson MichaelMerlin L RobbMary MarovichJerome H KimWe performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody.http://europepmc.org/articles/PMC4321833?pdf=render |
| spellingShingle | Silvia Ratto-Kim Mark S de Souza Jeffrey R Currier Nicos Karasavvas John Sidney Morgane Rolland Anais Valencia-Micolta Sirinan Madnote Alessandro Sette Sorachai Nitayaphan Punnee Pitisuttuthum Jaranit Kaewkungwal Supachai Rerks-Ngarm Robert O'Connell Nelson Michael Merlin L Robb Mary Marovich Jerome H Kim Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E. PLoS ONE |
| title | Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E. |
| title_full | Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E. |
| title_fullStr | Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E. |
| title_full_unstemmed | Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E. |
| title_short | Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E. |
| title_sort | identification of immunodominant cd4 restricted epitopes co located with antibody binding sites in individuals vaccinated with alvac hiv and aidsvax b e |
| url | http://europepmc.org/articles/PMC4321833?pdf=render |
| work_keys_str_mv | AT silviarattokim identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT marksdesouza identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT jeffreyrcurrier identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT nicoskarasavvas identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT johnsidney identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT morganerolland identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT anaisvalenciamicolta identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT sirinanmadnote identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT alessandrosette identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT sorachainitayaphan identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT punneepitisuttuthum identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT jaranitkaewkungwal identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT supachairerksngarm identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT robertoconnell identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT nelsonmichael identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT merlinlrobb identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT marymarovich identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe AT jeromehkim identificationofimmunodominantcd4restrictedepitopescolocatedwithantibodybindingsitesinindividualsvaccinatedwithalvachivandaidsvaxbe |