Sensitive colorimetric assay using insulin G-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticles
Described here is a methodology for fabrication of a sensitive colorimetric nanoassay for measurement of insulin using G-quadruplex aptamer arrays on DNA nanotubes (DNTs) coupled with magnetic nanoparticles. The spectroscopic findings (e.g. visible spectra, velocity assay and limit of detection dete...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2018-01-01
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| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171835 |
| _version_ | 1818348629198372864 |
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| author | A. Rafati A. Zarrabi S. Abediankenari M. Aarabi P. Gill |
| author_facet | A. Rafati A. Zarrabi S. Abediankenari M. Aarabi P. Gill |
| author_sort | A. Rafati |
| collection | DOAJ |
| description | Described here is a methodology for fabrication of a sensitive colorimetric nanoassay for measurement of insulin using G-quadruplex aptamer arrays on DNA nanotubes (DNTs) coupled with magnetic nanoparticles. The spectroscopic findings (e.g. visible spectra, velocity assay and limit of detection determination) indicated a highly sensitive performance of this new nanoassay in comparison to those results obtained from the insulin assay with non-arrayed aptamers. The clinical performance statistics (i.e. paired sample t-test, Bland–Altman plot and scatter diagram) from the newly developed assay and the enzyme-linked immunosorbent assay suggested its reliable precision and its acceptable repeatability for measurement of insulin in human sera. This is, to our knowledge, the first study for the application of magnetic nanoparticle-coupled DNTs for carrying G-quadruplex aptamers for detection of biomolecules (such as insulin) in human serum. |
| first_indexed | 2024-12-13T17:53:05Z |
| format | Article |
| id | doaj.art-3f4b2c3dc5e043608e5921e6ea1f3be3 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-13T17:53:05Z |
| publishDate | 2018-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-3f4b2c3dc5e043608e5921e6ea1f3be32022-12-21T23:36:27ZengThe Royal SocietyRoyal Society Open Science2054-57032018-01-015310.1098/rsos.171835171835Sensitive colorimetric assay using insulin G-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticlesA. RafatiA. ZarrabiS. AbediankenariM. AarabiP. GillDescribed here is a methodology for fabrication of a sensitive colorimetric nanoassay for measurement of insulin using G-quadruplex aptamer arrays on DNA nanotubes (DNTs) coupled with magnetic nanoparticles. The spectroscopic findings (e.g. visible spectra, velocity assay and limit of detection determination) indicated a highly sensitive performance of this new nanoassay in comparison to those results obtained from the insulin assay with non-arrayed aptamers. The clinical performance statistics (i.e. paired sample t-test, Bland–Altman plot and scatter diagram) from the newly developed assay and the enzyme-linked immunosorbent assay suggested its reliable precision and its acceptable repeatability for measurement of insulin in human sera. This is, to our knowledge, the first study for the application of magnetic nanoparticle-coupled DNTs for carrying G-quadruplex aptamers for detection of biomolecules (such as insulin) in human serum.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171835g-quadruplex aptamermnp-coupled dna nanotubeinsulin colorimetric assay |
| spellingShingle | A. Rafati A. Zarrabi S. Abediankenari M. Aarabi P. Gill Sensitive colorimetric assay using insulin G-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticles Royal Society Open Science g-quadruplex aptamer mnp-coupled dna nanotube insulin colorimetric assay |
| title | Sensitive colorimetric assay using insulin G-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticles |
| title_full | Sensitive colorimetric assay using insulin G-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticles |
| title_fullStr | Sensitive colorimetric assay using insulin G-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticles |
| title_full_unstemmed | Sensitive colorimetric assay using insulin G-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticles |
| title_short | Sensitive colorimetric assay using insulin G-quadruplex aptamer arrays on DNA nanotubes coupled with magnetic nanoparticles |
| title_sort | sensitive colorimetric assay using insulin g quadruplex aptamer arrays on dna nanotubes coupled with magnetic nanoparticles |
| topic | g-quadruplex aptamer mnp-coupled dna nanotube insulin colorimetric assay |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171835 |
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