Fungicidal activity of volatile organic compounds from Paenibacillus bacteria against Colletotrichum gloeosporioides
Some bacteria release volatile organic compounds (VOCs) that can influence the growth of other microorganisms including some pathogens. Identifying bacteria with antifungal activity makes it possible to use such bacteria in the development of biocontrol agents. Thus, the present study focuses on scr...
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| Format: | Article |
| Language: | English |
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Universidad Nacional de Colombia
2020-01-01
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| Series: | Revista Colombiana de Química |
| Subjects: | |
| Online Access: | https://revistas.unal.edu.co/index.php/rcolquim/article/view/81996 |
| _version_ | 1819143850992599040 |
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| author | Laura Carolina Coconubo Guio Diana Cristina Sinuco León Leonardo Castellanos Hernández |
| author_facet | Laura Carolina Coconubo Guio Diana Cristina Sinuco León Leonardo Castellanos Hernández |
| author_sort | Laura Carolina Coconubo Guio |
| collection | DOAJ |
| description | Some bacteria release volatile organic compounds (VOCs) that can influence the growth of other microorganisms including some pathogens. Identifying bacteria with antifungal activity makes it possible to use such bacteria in the development of biocontrol agents. Thus, the present study focuses on screening VOCs released by eight isolates from Paenibacillus genus, collected at Old Providence and Santa Catalina coral reef (Colombian Caribbean Sea), with antifungal activity against phytopathogenic fungi Colletotrichum gloeosporioides 26B. The VOCs from Paenibacillus sp (PNM-50) showed inhibition rates higher than 50% in the mycelial fungi growth accompanied by macroscopic morphological changes and a reduction in conidiation. In order to identify the VOCs responsible for this antifungal bioactivity, Headspace-Solid Phase Microextraction (HS-SPME) from the bacterial culture was conducted, followed by Gas Chromatography Mass Spectrometry (GC-MS). The chromatographic results revealed a high abundance of VOCs released just by culture media. Once, the difference between VOCs emitted by culture media and bacteria was established, it was possible to make a putative identification of 2-furanmethanol, phenylacetonitrile, and 2,4-dimethylpentanol as possible VOCs responsible for the antifungal activity. |
| first_indexed | 2024-12-22T12:32:48Z |
| format | Article |
| id | doaj.art-3f4b892903184075a7696720dfdf4efb |
| institution | Directory Open Access Journal |
| issn | 0120-2804 2357-3791 |
| language | English |
| last_indexed | 2024-12-22T12:32:48Z |
| publishDate | 2020-01-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Química |
| spelling | doaj.art-3f4b892903184075a7696720dfdf4efb2022-12-21T18:25:37ZengUniversidad Nacional de ColombiaRevista Colombiana de Química0120-28042357-37912020-01-01491202510.15446/rev.colomb.quim.v1n49.8199652141Fungicidal activity of volatile organic compounds from Paenibacillus bacteria against Colletotrichum gloeosporioidesLaura Carolina Coconubo GuioDiana Cristina Sinuco LeónLeonardo Castellanos HernándezSome bacteria release volatile organic compounds (VOCs) that can influence the growth of other microorganisms including some pathogens. Identifying bacteria with antifungal activity makes it possible to use such bacteria in the development of biocontrol agents. Thus, the present study focuses on screening VOCs released by eight isolates from Paenibacillus genus, collected at Old Providence and Santa Catalina coral reef (Colombian Caribbean Sea), with antifungal activity against phytopathogenic fungi Colletotrichum gloeosporioides 26B. The VOCs from Paenibacillus sp (PNM-50) showed inhibition rates higher than 50% in the mycelial fungi growth accompanied by macroscopic morphological changes and a reduction in conidiation. In order to identify the VOCs responsible for this antifungal bioactivity, Headspace-Solid Phase Microextraction (HS-SPME) from the bacterial culture was conducted, followed by Gas Chromatography Mass Spectrometry (GC-MS). The chromatographic results revealed a high abundance of VOCs released just by culture media. Once, the difference between VOCs emitted by culture media and bacteria was established, it was possible to make a putative identification of 2-furanmethanol, phenylacetonitrile, and 2,4-dimethylpentanol as possible VOCs responsible for the antifungal activity.https://revistas.unal.edu.co/index.php/rcolquim/article/view/81996marine bacteriaantifungal activityhs-spme-gc-ms. |
| spellingShingle | Laura Carolina Coconubo Guio Diana Cristina Sinuco León Leonardo Castellanos Hernández Fungicidal activity of volatile organic compounds from Paenibacillus bacteria against Colletotrichum gloeosporioides Revista Colombiana de Química marine bacteria antifungal activity hs-spme-gc-ms. |
| title | Fungicidal activity of volatile organic compounds from Paenibacillus bacteria against Colletotrichum gloeosporioides |
| title_full | Fungicidal activity of volatile organic compounds from Paenibacillus bacteria against Colletotrichum gloeosporioides |
| title_fullStr | Fungicidal activity of volatile organic compounds from Paenibacillus bacteria against Colletotrichum gloeosporioides |
| title_full_unstemmed | Fungicidal activity of volatile organic compounds from Paenibacillus bacteria against Colletotrichum gloeosporioides |
| title_short | Fungicidal activity of volatile organic compounds from Paenibacillus bacteria against Colletotrichum gloeosporioides |
| title_sort | fungicidal activity of volatile organic compounds from paenibacillus bacteria against colletotrichum gloeosporioides |
| topic | marine bacteria antifungal activity hs-spme-gc-ms. |
| url | https://revistas.unal.edu.co/index.php/rcolquim/article/view/81996 |
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