SIMPLE AND SENSITIVE SPECTROPHOTO¬METRIC ASSAY OF OFLOXACIN IN PHARMACEUTICALS BASED ON ION-PAIR REACTION
Two simple, sensitive, economical and extraction-free spectrophotometric methods have been developed for the determination of ofloxacin (OFX) in pure form and in tablets. The methods are based on the interaction of OFX with two sulphonphthalein dyes, namely, bromothymol blue (method A) and bromophen...
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| Format: | Article |
| Language: | English |
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Association of the Chemical Engineers of Serbia
2010-12-01
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| Series: | Chemical Industry and Chemical Engineering Quarterly |
| Subjects: | |
| Online Access: | http://www.ache.org.rs/CICEQ/2010/No4/CICEQ_Vol16_%20No4_pp353-362_Oct-Dec_2010.pdf |
| _version_ | 1828236521254158336 |
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| author | K.B. VINAY O. ZENITA DEVI K. BASAVAIAH P.J. RAMESH |
| author_facet | K.B. VINAY O. ZENITA DEVI K. BASAVAIAH P.J. RAMESH |
| author_sort | K.B. VINAY |
| collection | DOAJ |
| description | Two simple, sensitive, economical and extraction-free spectrophotometric methods have been developed for the determination of ofloxacin (OFX) in pure form and in tablets. The methods are based on the interaction of OFX with two sulphonphthalein dyes, namely, bromothymol blue (method A) and bromophenol blue (method B) in dichloromethane medium to form stable, yellow-colored ion–pair complexes peaking at 410 nm. Under the optimum conditions, OFX could be assayed in the concentration ranges 1.25-20 and 1.0-16 µg mL-1 OFX by methods A and B, respectively, with correlation coefficient of 0.999 in both methods. The apparent molar absorptivity values are calculated to be 1.74104 and 2.18104, L moL-1 cm-1, for method A and B, respectively, with corres¬pond¬ing Sandell sensitivity values of 0.021 and 0.017 µg cm-2. The limits of detec¬tion (LOD) and quantification (LOQ) are also reported. The stoichiometry of the reaction was found to be 1:1 in both cases and the conditional stability cons¬tants (Kf) of the complexes have also been reported. The intra-day and inter-day variation was assessed. The methods were applied to determine OFX from marked tablet formulations. Statistical analysis proved that the proposed methods were both accurate and precise. |
| first_indexed | 2024-04-12T20:33:33Z |
| format | Article |
| id | doaj.art-3f4ea00ceaeb455b8fb2d5ec4597face |
| institution | Directory Open Access Journal |
| issn | 1451-9372 |
| language | English |
| last_indexed | 2024-04-12T20:33:33Z |
| publishDate | 2010-12-01 |
| publisher | Association of the Chemical Engineers of Serbia |
| record_format | Article |
| series | Chemical Industry and Chemical Engineering Quarterly |
| spelling | doaj.art-3f4ea00ceaeb455b8fb2d5ec4597face2022-12-22T03:17:39ZengAssociation of the Chemical Engineers of SerbiaChemical Industry and Chemical Engineering Quarterly1451-93722010-12-01164353362SIMPLE AND SENSITIVE SPECTROPHOTO¬METRIC ASSAY OF OFLOXACIN IN PHARMACEUTICALS BASED ON ION-PAIR REACTIONK.B. VINAYO. ZENITA DEVIK. BASAVAIAHP.J. RAMESHTwo simple, sensitive, economical and extraction-free spectrophotometric methods have been developed for the determination of ofloxacin (OFX) in pure form and in tablets. The methods are based on the interaction of OFX with two sulphonphthalein dyes, namely, bromothymol blue (method A) and bromophenol blue (method B) in dichloromethane medium to form stable, yellow-colored ion–pair complexes peaking at 410 nm. Under the optimum conditions, OFX could be assayed in the concentration ranges 1.25-20 and 1.0-16 µg mL-1 OFX by methods A and B, respectively, with correlation coefficient of 0.999 in both methods. The apparent molar absorptivity values are calculated to be 1.74104 and 2.18104, L moL-1 cm-1, for method A and B, respectively, with corres¬pond¬ing Sandell sensitivity values of 0.021 and 0.017 µg cm-2. The limits of detec¬tion (LOD) and quantification (LOQ) are also reported. The stoichiometry of the reaction was found to be 1:1 in both cases and the conditional stability cons¬tants (Kf) of the complexes have also been reported. The intra-day and inter-day variation was assessed. The methods were applied to determine OFX from marked tablet formulations. Statistical analysis proved that the proposed methods were both accurate and precise.http://www.ache.org.rs/CICEQ/2010/No4/CICEQ_Vol16_%20No4_pp353-362_Oct-Dec_2010.pdfdeterminationsulphonphthalein dyesion-pair |
| spellingShingle | K.B. VINAY O. ZENITA DEVI K. BASAVAIAH P.J. RAMESH SIMPLE AND SENSITIVE SPECTROPHOTO¬METRIC ASSAY OF OFLOXACIN IN PHARMACEUTICALS BASED ON ION-PAIR REACTION Chemical Industry and Chemical Engineering Quarterly determination sulphonphthalein dyes ion-pair |
| title | SIMPLE AND SENSITIVE SPECTROPHOTO¬METRIC ASSAY OF OFLOXACIN IN PHARMACEUTICALS BASED ON ION-PAIR REACTION |
| title_full | SIMPLE AND SENSITIVE SPECTROPHOTO¬METRIC ASSAY OF OFLOXACIN IN PHARMACEUTICALS BASED ON ION-PAIR REACTION |
| title_fullStr | SIMPLE AND SENSITIVE SPECTROPHOTO¬METRIC ASSAY OF OFLOXACIN IN PHARMACEUTICALS BASED ON ION-PAIR REACTION |
| title_full_unstemmed | SIMPLE AND SENSITIVE SPECTROPHOTO¬METRIC ASSAY OF OFLOXACIN IN PHARMACEUTICALS BASED ON ION-PAIR REACTION |
| title_short | SIMPLE AND SENSITIVE SPECTROPHOTO¬METRIC ASSAY OF OFLOXACIN IN PHARMACEUTICALS BASED ON ION-PAIR REACTION |
| title_sort | simple and sensitive spectrophoto¬metric assay of ofloxacin in pharmaceuticals based on ion pair reaction |
| topic | determination sulphonphthalein dyes ion-pair |
| url | http://www.ache.org.rs/CICEQ/2010/No4/CICEQ_Vol16_%20No4_pp353-362_Oct-Dec_2010.pdf |
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