Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and Development
Salvia miltiorrhiza Bunge (S. miltiorrhiza), a traditional Chinese medicinal herb, contains numerous bioactive components with broad range of pharmacological properties. By increasing the levels of endogenous jasmonate (JA) in plants or treating them with methyl jasmonate (MeJA), the level of tanshi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.630424/full |
| _version_ | 1819284234556145664 |
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| author | Lin Li Yuanchu Liu Ying Huang Bin Li Wen Ma Donghao Wang Xiaoyan Cao Zhezhi Wang |
| author_facet | Lin Li Yuanchu Liu Ying Huang Bin Li Wen Ma Donghao Wang Xiaoyan Cao Zhezhi Wang |
| author_sort | Lin Li |
| collection | DOAJ |
| description | Salvia miltiorrhiza Bunge (S. miltiorrhiza), a traditional Chinese medicinal herb, contains numerous bioactive components with broad range of pharmacological properties. By increasing the levels of endogenous jasmonate (JA) in plants or treating them with methyl jasmonate (MeJA), the level of tanshinones and salvianolic acids can be greatly enhanced. The jasmonate ZIM (JAZ) proteins belong to the TIFY family, and act as repressors, releasing targeted transcriptional factors in the JA signaling pathway. Herein, we identified and characterized 15 TIFY proteins present in S. miltiorrhiza. Quantitative reverse transcription PCR analysis indicated that the JAZ genes were all constitutively expressed in different tissues and were induced by MeJA treatments. SmJAZ3, which negatively regulates the tanshinones biosynthesis pathway in S. miltiorrhiza and the detailed molecular mechanism is poorly understood. SmJAZ3 acts as a bait protein to capture and identify a WD-repeat containing the protein SmWD40-170. Further molecular and genetic analysis revealed that SmWD40-170 is a positive regulator, promoting the accumulation of secondary metabolites in S. miltiorrhiza. Our study systematically analyzed the TIFY family and speculated a module of the JAZ-WD40 complex provides new insights into the mechanisms regulating the biosynthesis of secondary metabolites in S. miltiorrhiza. |
| first_indexed | 2024-12-24T01:44:08Z |
| format | Article |
| id | doaj.art-3f5469e5594846d684b8c8250eb35b78 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-24T01:44:08Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3f5469e5594846d684b8c8250eb35b782022-12-21T17:21:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-02-011210.3389/fpls.2021.630424630424Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and DevelopmentLin LiYuanchu LiuYing HuangBin LiWen MaDonghao WangXiaoyan CaoZhezhi WangSalvia miltiorrhiza Bunge (S. miltiorrhiza), a traditional Chinese medicinal herb, contains numerous bioactive components with broad range of pharmacological properties. By increasing the levels of endogenous jasmonate (JA) in plants or treating them with methyl jasmonate (MeJA), the level of tanshinones and salvianolic acids can be greatly enhanced. The jasmonate ZIM (JAZ) proteins belong to the TIFY family, and act as repressors, releasing targeted transcriptional factors in the JA signaling pathway. Herein, we identified and characterized 15 TIFY proteins present in S. miltiorrhiza. Quantitative reverse transcription PCR analysis indicated that the JAZ genes were all constitutively expressed in different tissues and were induced by MeJA treatments. SmJAZ3, which negatively regulates the tanshinones biosynthesis pathway in S. miltiorrhiza and the detailed molecular mechanism is poorly understood. SmJAZ3 acts as a bait protein to capture and identify a WD-repeat containing the protein SmWD40-170. Further molecular and genetic analysis revealed that SmWD40-170 is a positive regulator, promoting the accumulation of secondary metabolites in S. miltiorrhiza. Our study systematically analyzed the TIFY family and speculated a module of the JAZ-WD40 complex provides new insights into the mechanisms regulating the biosynthesis of secondary metabolites in S. miltiorrhiza.https://www.frontiersin.org/articles/10.3389/fpls.2021.630424/fullSalvia miltiorrhizajasmonateTIFY proteinsSmJAZ3SmWD40-170 |
| spellingShingle | Lin Li Yuanchu Liu Ying Huang Bin Li Wen Ma Donghao Wang Xiaoyan Cao Zhezhi Wang Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and Development Frontiers in Plant Science Salvia miltiorrhiza jasmonate TIFY proteins SmJAZ3 SmWD40-170 |
| title | Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and Development |
| title_full | Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and Development |
| title_fullStr | Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and Development |
| title_full_unstemmed | Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and Development |
| title_short | Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and Development |
| title_sort | genome wide identification of the tify family in salvia miltiorrhiza reveals that smjaz3 interacts with smwd40 170 a relevant protein that modulates secondary metabolism and development |
| topic | Salvia miltiorrhiza jasmonate TIFY proteins SmJAZ3 SmWD40-170 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.630424/full |
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