Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis
Burkholderia contaminans, an emerging pathogen related to cystic fibrosis, is known to cause potentially fatal infections in humans and ruminants, especially in immunocompromised individuals. However, the immune responses in cows following its infection have not been fully elucidated. In this study,...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-03-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1099623/full |
| _version_ | 1811158902941679616 |
|---|---|
| author | Zhipeng Zhang Zhipeng Zhang Yiyang Yao Jiayu Yang Hui Jiang Ye Meng Wenqiang Cao Fuzhen Zhou Kun Wang Zhangping Yang Zhangping Yang Chunhua Yang Jie Sun Yi Yang Yi Yang |
| author_facet | Zhipeng Zhang Zhipeng Zhang Yiyang Yao Jiayu Yang Hui Jiang Ye Meng Wenqiang Cao Fuzhen Zhou Kun Wang Zhangping Yang Zhangping Yang Chunhua Yang Jie Sun Yi Yang Yi Yang |
| author_sort | Zhipeng Zhang |
| collection | DOAJ |
| description | Burkholderia contaminans, an emerging pathogen related to cystic fibrosis, is known to cause potentially fatal infections in humans and ruminants, especially in immunocompromised individuals. However, the immune responses in cows following its infection have not been fully elucidated. In this study, T- and B-lymphocytes-mediated immune responses were evaluated in 15 B. contaminans-induced mastitis cows and 15 healthy cows with multi-parameter flow cytometry. The results showed that infection with B. contaminans was associated with a significant decrease in the number and percentage of B lymphocytes but with a significant increase in the proportion of IgG+CD27+ B lymphocytes. This indicated that humoral immune response may not be adequate to fight intracellular infection, which could contribute to the persistent bacterial infection. In addition, B. contaminans infection induced significant increase of γδ T cells and double positive (DP) CD4+CD8+ T cells but not CD4+ or CD8+ (single positive) T cells in blood. Phenotypic analysis showed that the percentages of activated WC1+ γδ T cells in peripheral blood were increased in the B. contaminans infected cows. Interestingly, intracellular cytokine staining showed that cattle naturally infected with B. contaminans exhibited multifunctional TNF-α+IFN-γ+IL-2+B. contaminans-specific DP T cells. Our results, for the first time, revealed a potential role of IgG+CD27+ B cells, CD4+CD8+ T cells and WC1+ γδ T cells in the defense of B. contaminans-induced mastitis in cows. |
| first_indexed | 2024-04-10T05:32:15Z |
| format | Article |
| id | doaj.art-3f561dafeb984b8bb8662b69a5251d40 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T05:32:15Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3f561dafeb984b8bb8662b69a5251d402023-03-07T05:58:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.10996231099623Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitisZhipeng Zhang0Zhipeng Zhang1Yiyang Yao2Jiayu Yang3Hui Jiang4Ye Meng5Wenqiang Cao6Fuzhen Zhou7Kun Wang8Zhangping Yang9Zhangping Yang10Chunhua Yang11Jie Sun12Yi Yang13Yi Yang14Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou, ChinaInstitute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, ChinaShenzhen Academy of Inspection and Quarantine Sciences, Shenzhen, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou, ChinaBurkholderia contaminans, an emerging pathogen related to cystic fibrosis, is known to cause potentially fatal infections in humans and ruminants, especially in immunocompromised individuals. However, the immune responses in cows following its infection have not been fully elucidated. In this study, T- and B-lymphocytes-mediated immune responses were evaluated in 15 B. contaminans-induced mastitis cows and 15 healthy cows with multi-parameter flow cytometry. The results showed that infection with B. contaminans was associated with a significant decrease in the number and percentage of B lymphocytes but with a significant increase in the proportion of IgG+CD27+ B lymphocytes. This indicated that humoral immune response may not be adequate to fight intracellular infection, which could contribute to the persistent bacterial infection. In addition, B. contaminans infection induced significant increase of γδ T cells and double positive (DP) CD4+CD8+ T cells but not CD4+ or CD8+ (single positive) T cells in blood. Phenotypic analysis showed that the percentages of activated WC1+ γδ T cells in peripheral blood were increased in the B. contaminans infected cows. Interestingly, intracellular cytokine staining showed that cattle naturally infected with B. contaminans exhibited multifunctional TNF-α+IFN-γ+IL-2+B. contaminans-specific DP T cells. Our results, for the first time, revealed a potential role of IgG+CD27+ B cells, CD4+CD8+ T cells and WC1+ γδ T cells in the defense of B. contaminans-induced mastitis in cows.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1099623/fulldairy cowsadaptive immune responsesBurkholderia contaminansmastitisflow cytometry |
| spellingShingle | Zhipeng Zhang Zhipeng Zhang Yiyang Yao Jiayu Yang Hui Jiang Ye Meng Wenqiang Cao Fuzhen Zhou Kun Wang Zhangping Yang Zhangping Yang Chunhua Yang Jie Sun Yi Yang Yi Yang Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis Frontiers in Microbiology dairy cows adaptive immune responses Burkholderia contaminans mastitis flow cytometry |
| title | Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis |
| title_full | Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis |
| title_fullStr | Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis |
| title_full_unstemmed | Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis |
| title_short | Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis |
| title_sort | assessment of adaptive immune responses of dairy cows with burkholderia contaminans induced mastitis |
| topic | dairy cows adaptive immune responses Burkholderia contaminans mastitis flow cytometry |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1099623/full |
| work_keys_str_mv | AT zhipengzhang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT zhipengzhang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT yiyangyao assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT jiayuyang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT huijiang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT yemeng assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT wenqiangcao assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT fuzhenzhou assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT kunwang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT zhangpingyang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT zhangpingyang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT chunhuayang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT jiesun assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT yiyang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis AT yiyang assessmentofadaptiveimmuneresponsesofdairycowswithburkholderiacontaminansinducedmastitis |