Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
Super-resolution fluorescent imaging typically makes use of intense phototoxic illumination. Here the authors achieve live-cell super-resolution imaging using low-illumination standard microscopes with the aid of a new analytical approach called Super-Resolution Radial Fluctuations (SRRF), provided...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2016-08-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms12471 |
| _version_ | 1818991478858317824 |
|---|---|
| author | Nils Gustafsson Siân Culley George Ashdown Dylan M. Owen Pedro Matos Pereira Ricardo Henriques |
| author_facet | Nils Gustafsson Siân Culley George Ashdown Dylan M. Owen Pedro Matos Pereira Ricardo Henriques |
| author_sort | Nils Gustafsson |
| collection | DOAJ |
| description | Super-resolution fluorescent imaging typically makes use of intense phototoxic illumination. Here the authors achieve live-cell super-resolution imaging using low-illumination standard microscopes with the aid of a new analytical approach called Super-Resolution Radial Fluctuations (SRRF), provided as an ImageJ plugin. |
| first_indexed | 2024-12-20T20:10:55Z |
| format | Article |
| id | doaj.art-3f58ca8cc60e4b8db69d99381d87fa14 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-20T20:10:55Z |
| publishDate | 2016-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-3f58ca8cc60e4b8db69d99381d87fa142022-12-21T19:27:48ZengNature PortfolioNature Communications2041-17232016-08-01711910.1038/ncomms12471Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuationsNils Gustafsson0Siân Culley1George Ashdown2Dylan M. Owen3Pedro Matos Pereira4Ricardo Henriques5Department of Cell and Developmental Biology, Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology, University College LondonDepartment of Cell and Developmental Biology, Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology, University College LondonDepartment of Physics, Randall Division of Cell and Molecular Biophysics, King’s College LondonDepartment of Physics, Randall Division of Cell and Molecular Biophysics, King’s College LondonDepartment of Cell and Developmental Biology, Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology, University College LondonDepartment of Cell and Developmental Biology, Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology, University College LondonSuper-resolution fluorescent imaging typically makes use of intense phototoxic illumination. Here the authors achieve live-cell super-resolution imaging using low-illumination standard microscopes with the aid of a new analytical approach called Super-Resolution Radial Fluctuations (SRRF), provided as an ImageJ plugin.https://doi.org/10.1038/ncomms12471 |
| spellingShingle | Nils Gustafsson Siân Culley George Ashdown Dylan M. Owen Pedro Matos Pereira Ricardo Henriques Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations Nature Communications |
| title | Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations |
| title_full | Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations |
| title_fullStr | Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations |
| title_full_unstemmed | Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations |
| title_short | Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations |
| title_sort | fast live cell conventional fluorophore nanoscopy with imagej through super resolution radial fluctuations |
| url | https://doi.org/10.1038/ncomms12471 |
| work_keys_str_mv | AT nilsgustafsson fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT sianculley fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT georgeashdown fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT dylanmowen fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT pedromatospereira fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations AT ricardohenriques fastlivecellconventionalfluorophorenanoscopywithimagejthroughsuperresolutionradialfluctuations |