A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination

The efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced in plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A <i>Nicotiana benthamiana</i> tissue culture system was used to obtain a stable production of the E...

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Main Authors: Youngmin Park, Yeonsu Oh, Miaomiao Wang, Llilianne Ganges, José Alejandro Bohórquez, Soohong Park, Sungmin Gu, Jungae Park, Sangmin Lee, Jongkook Kim, Eun-Ju Sohn
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Vaccines
Subjects:
Online Access:https://www.mdpi.com/2076-393X/9/5/418
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author Youngmin Park
Yeonsu Oh
Miaomiao Wang
Llilianne Ganges
José Alejandro Bohórquez
Soohong Park
Sungmin Gu
Jungae Park
Sangmin Lee
Jongkook Kim
Eun-Ju Sohn
author_facet Youngmin Park
Yeonsu Oh
Miaomiao Wang
Llilianne Ganges
José Alejandro Bohórquez
Soohong Park
Sungmin Gu
Jungae Park
Sangmin Lee
Jongkook Kim
Eun-Ju Sohn
author_sort Youngmin Park
collection DOAJ
description The efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced in plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A <i>Nicotiana benthamiana</i> tissue culture system was used to obtain a stable production of the E2-glycoprotein fused to the porcine Fc region of IgG. Ten pregnant sows were divided into three groups: Groups 1 and 2 (four sows each) were vaccinated with either 100 μg/dose or 300 μg/dose of the subunit vaccine at 64 days of pregnancy. Group 3 (two sows) was injected with PBS. Groups 1 and 2 were boosted with the same vaccine dose. At 10 days post second vaccination, the sows in Groups 2 and 3 were challenged with a highly virulent CSFV strain. The vaccinated sows remained clinically healthy and seroconverted rapidly, showing efficient neutralizing antibodies. The fetuses from vaccinated sows did not show gross lesions, and all analyzed tissue samples tested negative for CSFV replication. However, fetuses of non-vaccinated sows had high CSFV replication in tested tissue samples. The results suggested that in vaccinated sows, the plant produced E2 marker vaccine induced the protective immunogenicity at challenge, leading to protection from vertical transmission to fetuses.
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spelling doaj.art-3f59d211ed73452ea8b91898c5c899392023-11-21T16:39:57ZengMDPI AGVaccines2076-393X2021-04-019541810.3390/vaccines9050418A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double VaccinationYoungmin Park0Yeonsu Oh1Miaomiao Wang2Llilianne Ganges3José Alejandro Bohórquez4Soohong Park5Sungmin Gu6Jungae Park7Sangmin Lee8Jongkook Kim9Eun-Ju Sohn10BioApplications Inc., Pohang 37668, KoreaCollege of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, KoreaOIE Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Barcelona, SpainOIE Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Barcelona, SpainOIE Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Barcelona, SpainBioApplications Inc., Pohang 37668, KoreaBioApplications Inc., Pohang 37668, KoreaBioApplications Inc., Pohang 37668, KoreaBioApplications Inc., Pohang 37668, KoreaBioApplications Inc., Pohang 37668, KoreaBioApplications Inc., Pohang 37668, KoreaThe efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced in plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A <i>Nicotiana benthamiana</i> tissue culture system was used to obtain a stable production of the E2-glycoprotein fused to the porcine Fc region of IgG. Ten pregnant sows were divided into three groups: Groups 1 and 2 (four sows each) were vaccinated with either 100 μg/dose or 300 μg/dose of the subunit vaccine at 64 days of pregnancy. Group 3 (two sows) was injected with PBS. Groups 1 and 2 were boosted with the same vaccine dose. At 10 days post second vaccination, the sows in Groups 2 and 3 were challenged with a highly virulent CSFV strain. The vaccinated sows remained clinically healthy and seroconverted rapidly, showing efficient neutralizing antibodies. The fetuses from vaccinated sows did not show gross lesions, and all analyzed tissue samples tested negative for CSFV replication. However, fetuses of non-vaccinated sows had high CSFV replication in tested tissue samples. The results suggested that in vaccinated sows, the plant produced E2 marker vaccine induced the protective immunogenicity at challenge, leading to protection from vertical transmission to fetuses.https://www.mdpi.com/2076-393X/9/5/418classical swine fever virusE2 glycoproteinmarker vaccinevertical transmissionplantprotection
spellingShingle Youngmin Park
Yeonsu Oh
Miaomiao Wang
Llilianne Ganges
José Alejandro Bohórquez
Soohong Park
Sungmin Gu
Jungae Park
Sangmin Lee
Jongkook Kim
Eun-Ju Sohn
A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination
Vaccines
classical swine fever virus
E2 glycoprotein
marker vaccine
vertical transmission
plant
protection
title A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination
title_full A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination
title_fullStr A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination
title_full_unstemmed A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination
title_short A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination
title_sort novel e2 glycoprotein subunit marker vaccine produced in plant is able to prevent classical swine fever virus vertical transmission after double vaccination
topic classical swine fever virus
E2 glycoprotein
marker vaccine
vertical transmission
plant
protection
url https://www.mdpi.com/2076-393X/9/5/418
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