Recognition of lyso-phospholipids by human natural killer T lymphocytes.
Natural killer T (NKT) cells are a subset of T lymphocytes with potent immunoregulatory properties. Recognition of self-antigens presented by CD1d molecules is an important route of NKT cell activation; however, the molecular identity of specific autoantigens that stimulate human NKT cells remains u...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-10-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC2760207?pdf=render |
| _version_ | 1819125233295032320 |
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| author | Lisa M Fox Daryl G Cox Jennifer L Lockridge Xiaohua Wang Xiuxu Chen Louise Scharf David L Trott Rachel M Ndonye Natacha Veerapen Gurdyal S Besra Amy R Howell Mark E Cook Erin J Adams William H Hildebrand Jenny E Gumperz |
| author_facet | Lisa M Fox Daryl G Cox Jennifer L Lockridge Xiaohua Wang Xiuxu Chen Louise Scharf David L Trott Rachel M Ndonye Natacha Veerapen Gurdyal S Besra Amy R Howell Mark E Cook Erin J Adams William H Hildebrand Jenny E Gumperz |
| author_sort | Lisa M Fox |
| collection | DOAJ |
| description | Natural killer T (NKT) cells are a subset of T lymphocytes with potent immunoregulatory properties. Recognition of self-antigens presented by CD1d molecules is an important route of NKT cell activation; however, the molecular identity of specific autoantigens that stimulate human NKT cells remains unclear. Here, we have analyzed human NKT cell recognition of CD1d cellular ligands. The most clearly antigenic species was lyso-phosphatidylcholine (LPC). Diacylated phosphatidylcholine and lyso-phosphoglycerols differing in the chemistry of the head group stimulated only weak responses from human NKT cells. However, lyso-sphingomyelin, which shares the phosphocholine head group of LPC, also activated NKT cells. Antigen-presenting cells pulsed with LPC were capable of stimulating increased cytokine responses by NKT cell clones and by freshly isolated peripheral blood lymphocytes. These results demonstrate that human NKT cells recognize cholinated lyso-phospholipids as antigens presented by CD1d. Since these lyso-phospholipids serve as lipid messengers in normal physiological processes and are present at elevated levels during inflammatory responses, these findings point to a novel link between NKT cells and cellular signaling pathways that are associated with human disease pathophysiology. |
| first_indexed | 2024-12-22T07:36:53Z |
| format | Article |
| id | doaj.art-3f5a22c8a7f44061b89d85e01ac33a7a |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-22T07:36:53Z |
| publishDate | 2009-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-3f5a22c8a7f44061b89d85e01ac33a7a2022-12-21T18:33:51ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852009-10-01710e100022810.1371/journal.pbio.1000228Recognition of lyso-phospholipids by human natural killer T lymphocytes.Lisa M FoxDaryl G CoxJennifer L LockridgeXiaohua WangXiuxu ChenLouise ScharfDavid L TrottRachel M NdonyeNatacha VeerapenGurdyal S BesraAmy R HowellMark E CookErin J AdamsWilliam H HildebrandJenny E GumperzNatural killer T (NKT) cells are a subset of T lymphocytes with potent immunoregulatory properties. Recognition of self-antigens presented by CD1d molecules is an important route of NKT cell activation; however, the molecular identity of specific autoantigens that stimulate human NKT cells remains unclear. Here, we have analyzed human NKT cell recognition of CD1d cellular ligands. The most clearly antigenic species was lyso-phosphatidylcholine (LPC). Diacylated phosphatidylcholine and lyso-phosphoglycerols differing in the chemistry of the head group stimulated only weak responses from human NKT cells. However, lyso-sphingomyelin, which shares the phosphocholine head group of LPC, also activated NKT cells. Antigen-presenting cells pulsed with LPC were capable of stimulating increased cytokine responses by NKT cell clones and by freshly isolated peripheral blood lymphocytes. These results demonstrate that human NKT cells recognize cholinated lyso-phospholipids as antigens presented by CD1d. Since these lyso-phospholipids serve as lipid messengers in normal physiological processes and are present at elevated levels during inflammatory responses, these findings point to a novel link between NKT cells and cellular signaling pathways that are associated with human disease pathophysiology.http://europepmc.org/articles/PMC2760207?pdf=render |
| spellingShingle | Lisa M Fox Daryl G Cox Jennifer L Lockridge Xiaohua Wang Xiuxu Chen Louise Scharf David L Trott Rachel M Ndonye Natacha Veerapen Gurdyal S Besra Amy R Howell Mark E Cook Erin J Adams William H Hildebrand Jenny E Gumperz Recognition of lyso-phospholipids by human natural killer T lymphocytes. PLoS Biology |
| title | Recognition of lyso-phospholipids by human natural killer T lymphocytes. |
| title_full | Recognition of lyso-phospholipids by human natural killer T lymphocytes. |
| title_fullStr | Recognition of lyso-phospholipids by human natural killer T lymphocytes. |
| title_full_unstemmed | Recognition of lyso-phospholipids by human natural killer T lymphocytes. |
| title_short | Recognition of lyso-phospholipids by human natural killer T lymphocytes. |
| title_sort | recognition of lyso phospholipids by human natural killer t lymphocytes |
| url | http://europepmc.org/articles/PMC2760207?pdf=render |
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