An early burst of IFN-γ induced by the pre-erythrocytic stage favours <it>Plasmodium yoelii </it>parasitaemia in B6 mice
<p>Abstract</p> <p>Background</p> <p>In murine models of malaria, an early proinflammatory response has been associated with the resolution of blood-stage infection. To dissect the protective immune mechanims that allow the control of parasitaemia, the early immune resp...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-06-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/8/1/128 |
| _version_ | 1811277553768333312 |
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| author | Barbier Eliane Gorgette Olivier Roland Jacques Soulard Valérie Cazenave Pierre-André Pied Sylviane |
| author_facet | Barbier Eliane Gorgette Olivier Roland Jacques Soulard Valérie Cazenave Pierre-André Pied Sylviane |
| author_sort | Barbier Eliane |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In murine models of malaria, an early proinflammatory response has been associated with the resolution of blood-stage infection. To dissect the protective immune mechanims that allow the control of parasitaemia, the early immune response of C57BL/6 mice induced during a non-lethal plasmodial infection was analysed.</p> <p>Methods</p> <p>Mice were infected with <it>Plasmodium yoelii </it>265BY sporozoites, the natural invasive form of the parasite, in order to complete its full life cycle. The concentrations of three proinflammatory cytokines in the sera of mice were determined by ELISA at different time points of infection. The contribution of the liver and the spleen to this cytokinic response was evaluated and the cytokine-producing lymphocytes were identified by flow cytometry. The physiological relevance of these results was tested by monitoring parasitaemia in genetically deficient C57BL/6 mice or wild-type mice treated with anti-cytokine neutralizing antibody. Finally, the cytokinic response in sera of mice infected with parasitized-RBCs was analysed.</p> <p>Results</p> <p>The early immune response of C57BL/6 mice to sporozoite-induced malaria is characterized by a peak of IFN-γ in the serum at day 5 of infection and splenic CD4 T lymphocytes are the major producer of this cytokine at this time point. Somewhat unexpected, the parasitaemia is significantly lower in <it>P. yoelii</it>-infected mice in the absence of IFN-γ. More precisely, at early time points of infection, IFN-γ favours parasitaemia, whereas helping to clear efficiently the blood-stage parasites at later time points. Interestingly, the early IFN-γ burst is induced by the pre-erythrocytic stage.</p> <p>Conclusion</p> <p>These results challenge the current view regarding the role of IFN-γ on the control of parasite growth since they show that IFN-γ is not an essential mediator of protection in <it>P. yoelii</it>-infected C57BL/6 mice. Moreover, the mice parasitaemia is more efficiently controlled in the absence of an early IFN-γ production, suggesting that this cytokine promotes parasite's growth. Finally, this early burst of IFN-γ is induced by the pre-erythrocytic stage, showing the impact of this stage on the immune response taking place during the subsequent erythrocytic stage.</p> |
| first_indexed | 2024-04-13T00:18:51Z |
| format | Article |
| id | doaj.art-3f5a9b271dbb41cc8d93977783f180f8 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-04-13T00:18:51Z |
| publishDate | 2009-06-01 |
| publisher | BMC |
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| series | Malaria Journal |
| spelling | doaj.art-3f5a9b271dbb41cc8d93977783f180f82022-12-22T03:10:50ZengBMCMalaria Journal1475-28752009-06-018112810.1186/1475-2875-8-128An early burst of IFN-γ induced by the pre-erythrocytic stage favours <it>Plasmodium yoelii </it>parasitaemia in B6 miceBarbier ElianeGorgette OlivierRoland JacquesSoulard ValérieCazenave Pierre-AndréPied Sylviane<p>Abstract</p> <p>Background</p> <p>In murine models of malaria, an early proinflammatory response has been associated with the resolution of blood-stage infection. To dissect the protective immune mechanims that allow the control of parasitaemia, the early immune response of C57BL/6 mice induced during a non-lethal plasmodial infection was analysed.</p> <p>Methods</p> <p>Mice were infected with <it>Plasmodium yoelii </it>265BY sporozoites, the natural invasive form of the parasite, in order to complete its full life cycle. The concentrations of three proinflammatory cytokines in the sera of mice were determined by ELISA at different time points of infection. The contribution of the liver and the spleen to this cytokinic response was evaluated and the cytokine-producing lymphocytes were identified by flow cytometry. The physiological relevance of these results was tested by monitoring parasitaemia in genetically deficient C57BL/6 mice or wild-type mice treated with anti-cytokine neutralizing antibody. Finally, the cytokinic response in sera of mice infected with parasitized-RBCs was analysed.</p> <p>Results</p> <p>The early immune response of C57BL/6 mice to sporozoite-induced malaria is characterized by a peak of IFN-γ in the serum at day 5 of infection and splenic CD4 T lymphocytes are the major producer of this cytokine at this time point. Somewhat unexpected, the parasitaemia is significantly lower in <it>P. yoelii</it>-infected mice in the absence of IFN-γ. More precisely, at early time points of infection, IFN-γ favours parasitaemia, whereas helping to clear efficiently the blood-stage parasites at later time points. Interestingly, the early IFN-γ burst is induced by the pre-erythrocytic stage.</p> <p>Conclusion</p> <p>These results challenge the current view regarding the role of IFN-γ on the control of parasite growth since they show that IFN-γ is not an essential mediator of protection in <it>P. yoelii</it>-infected C57BL/6 mice. Moreover, the mice parasitaemia is more efficiently controlled in the absence of an early IFN-γ production, suggesting that this cytokine promotes parasite's growth. Finally, this early burst of IFN-γ is induced by the pre-erythrocytic stage, showing the impact of this stage on the immune response taking place during the subsequent erythrocytic stage.</p>http://www.malariajournal.com/content/8/1/128 |
| spellingShingle | Barbier Eliane Gorgette Olivier Roland Jacques Soulard Valérie Cazenave Pierre-André Pied Sylviane An early burst of IFN-γ induced by the pre-erythrocytic stage favours <it>Plasmodium yoelii </it>parasitaemia in B6 mice Malaria Journal |
| title | An early burst of IFN-γ induced by the pre-erythrocytic stage favours <it>Plasmodium yoelii </it>parasitaemia in B6 mice |
| title_full | An early burst of IFN-γ induced by the pre-erythrocytic stage favours <it>Plasmodium yoelii </it>parasitaemia in B6 mice |
| title_fullStr | An early burst of IFN-γ induced by the pre-erythrocytic stage favours <it>Plasmodium yoelii </it>parasitaemia in B6 mice |
| title_full_unstemmed | An early burst of IFN-γ induced by the pre-erythrocytic stage favours <it>Plasmodium yoelii </it>parasitaemia in B6 mice |
| title_short | An early burst of IFN-γ induced by the pre-erythrocytic stage favours <it>Plasmodium yoelii </it>parasitaemia in B6 mice |
| title_sort | early burst of ifn γ induced by the pre erythrocytic stage favours it plasmodium yoelii it parasitaemia in b6 mice |
| url | http://www.malariajournal.com/content/8/1/128 |
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