BMP-15 m-RNA expression of mouse oocytes in vitro maturation in different droplet medium volume
Objective: To investigate droplet medium volume effect on the BMP-15 m-RNA expression. Methods: Oocytes are collected from mice ovaries by puncturing with a sterile 26-G needle. The droplet medium volumes are using 50 μL, 100 μL and 200 μL. The BMP-15 m-RNA expression is determined in each group. Re...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2015-12-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2305050015000202 |
| Summary: | Objective: To investigate droplet medium volume effect on the BMP-15 m-RNA expression.
Methods: Oocytes are collected from mice ovaries by puncturing with a sterile 26-G needle. The droplet medium volumes are using 50 μL, 100 μL and 200 μL. The BMP-15 m-RNA expression is determined in each group.
Results: The results indicated that BMP-15 m-RNA expression did not significantly difference when oocyte were cultured in 50 and 100 μL/droplet medium volume, but significantly difference (P < 0.05) when oocytes were cultured in 200 μL/droplet medium volume.
Conclusions: The highest BMP-15 m-RNA expression occurs when oocytes are cultured in 200 μL/droplet medium volume. |
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| ISSN: | 2305-0500 |