Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas
Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2020-06-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/9180.pdf |
| _version_ | 1827606003862994944 |
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| author | Robert W.A. Potts Alejandro P. Gutierrez Yennifer Cortés-Araya Ross D. Houston Tim P. Bean |
| author_facet | Robert W.A. Potts Alejandro P. Gutierrez Yennifer Cortés-Araya Ross D. Houston Tim P. Bean |
| author_sort | Robert W.A. Potts |
| collection | DOAJ |
| description | Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease. |
| first_indexed | 2024-03-09T06:31:51Z |
| format | Article |
| id | doaj.art-3f66fe8a46b0498ba7760e353e22fcba |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:31:51Z |
| publishDate | 2020-06-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-3f66fe8a46b0498ba7760e353e22fcba2023-12-03T11:04:22ZengPeerJ Inc.PeerJ2167-83592020-06-018e918010.7717/peerj.9180Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigasRobert W.A. Potts0Alejandro P. Gutierrez1Yennifer Cortés-Araya2Ross D. Houston3Tim P. Bean4The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United KingdomCell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease.https://peerj.com/articles/9180.pdfPrimary cell culturePacific oysterTissue explantLive cell imaging |
| spellingShingle | Robert W.A. Potts Alejandro P. Gutierrez Yennifer Cortés-Araya Ross D. Houston Tim P. Bean Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas PeerJ Primary cell culture Pacific oyster Tissue explant Live cell imaging |
| title | Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
| title_full | Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
| title_fullStr | Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
| title_full_unstemmed | Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
| title_short | Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
| title_sort | developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve crassostrea gigas |
| topic | Primary cell culture Pacific oyster Tissue explant Live cell imaging |
| url | https://peerj.com/articles/9180.pdf |
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