ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons.

ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons.

The correct subcellular distribution of proteins establishes the complex morphology and function of neurons. Fluorescence microscopy techniques are invaluable to investigate subcellular protein distribution, but they suffer from the limited ability to efficiently and reliably label endogenous protei...

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Bibliographic Details
Main Authors:	Jelmer Willems, Arthur P H de Jong, Nicky Scheefhals, Eline Mertens, Lisa A E Catsburg, Rogier B Poorthuis, Fred de Winter, Joost Verhaagen, Frank J Meye, Harold D MacGillavry
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2020-04-01
Series:	PLoS Biology
Online Access:	https://doi.org/10.1371/journal.pbio.3000665

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