Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysis
Traditionally, in the cigarettes industry, the determination of ammonium ion in the mainstream smoke is performed by ion chromatography. This work studies this determination and compares the results of this technique with the use of external and internal standard calibration. A reference cigarette s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2010-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000400041 |
| _version_ | 1811312175478734848 |
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| author | Elcio Cruz de Oliveira Edson I. Muller Fernanda Abad Juliana Dallarosa Cristine Adriano |
| author_facet | Elcio Cruz de Oliveira Edson I. Muller Fernanda Abad Juliana Dallarosa Cristine Adriano |
| author_sort | Elcio Cruz de Oliveira |
| collection | DOAJ |
| description | Traditionally, in the cigarettes industry, the determination of ammonium ion in the mainstream smoke is performed by ion chromatography. This work studies this determination and compares the results of this technique with the use of external and internal standard calibration. A reference cigarette sample presented measurement uncertainty of 2.0 μg/cigarette and 1.5 μg/cigarette, with external and internal standard, respectively. It is observed that the greatest source of uncertainty is the bias correction factor and that it is even more significant when using external standard, confirming thus the importance of internal standardization for this correction. |
| first_indexed | 2024-04-13T10:31:25Z |
| format | Article |
| id | doaj.art-3f757937b8cc40f593319d7ae05d091f |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-04-13T10:31:25Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-3f757937b8cc40f593319d7ae05d091f2022-12-22T02:50:09ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642010-01-0133498498710.1590/S0100-40422010000400041Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysisElcio Cruz de OliveiraEdson I. MullerFernanda AbadJuliana DallarosaCristine AdrianoTraditionally, in the cigarettes industry, the determination of ammonium ion in the mainstream smoke is performed by ion chromatography. This work studies this determination and compares the results of this technique with the use of external and internal standard calibration. A reference cigarette sample presented measurement uncertainty of 2.0 μg/cigarette and 1.5 μg/cigarette, with external and internal standard, respectively. It is observed that the greatest source of uncertainty is the bias correction factor and that it is even more significant when using external standard, confirming thus the importance of internal standardization for this correction.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000400041internal and external standard calibrationmeasurement uncertaintybias correction factor |
| spellingShingle | Elcio Cruz de Oliveira Edson I. Muller Fernanda Abad Juliana Dallarosa Cristine Adriano Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysis Química Nova internal and external standard calibration measurement uncertainty bias correction factor |
| title | Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysis |
| title_full | Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysis |
| title_fullStr | Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysis |
| title_full_unstemmed | Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysis |
| title_short | Internal standard versus external standard calibration: an uncertainty case study of a liquid chromatography analysis |
| title_sort | internal standard versus external standard calibration an uncertainty case study of a liquid chromatography analysis |
| topic | internal and external standard calibration measurement uncertainty bias correction factor |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000400041 |
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