Astrovirus Diagnostics

Various methods exist to detect an astrovirus infection. Current methods include electron microscopy (EM), cell culture, immunoassays, polymerase chain reaction (PCR) and various other molecular approaches that can be applied in the context of diagnostic or in surveillance studies. With the advent o...

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Main Authors: Philippe Pérot, Marc Lecuit, Marc Eloit
Format: Article
Language:English
Published: MDPI AG 2017-01-01
Series:Viruses
Subjects:
Online Access:http://www.mdpi.com/1999-4915/9/1/10
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author Philippe Pérot
Marc Lecuit
Marc Eloit
author_facet Philippe Pérot
Marc Lecuit
Marc Eloit
author_sort Philippe Pérot
collection DOAJ
description Various methods exist to detect an astrovirus infection. Current methods include electron microscopy (EM), cell culture, immunoassays, polymerase chain reaction (PCR) and various other molecular approaches that can be applied in the context of diagnostic or in surveillance studies. With the advent of metagenomics, novel human astrovirus (HAstV) strains have been found in immunocompromised individuals in association with central nervous system (CNS) infections. This work reviews the past and current methods for astrovirus detection and their uses in both research laboratories and for medical diagnostic purposes.
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spelling doaj.art-3f76f15d552944faad805917e7252be62022-12-22T02:20:08ZengMDPI AGViruses1999-49152017-01-01911010.3390/v9010010v9010010Astrovirus DiagnosticsPhilippe Pérot0Marc Lecuit1Marc Eloit2Institut Pasteur, Biology of Infection Unit, Inserm U1117, Laboratory of Pathogen Discovery, 75015 Paris, FranceInstitut Pasteur, Biology of Infection Unit, Inserm U1117, Laboratory of Pathogen Discovery, 75015 Paris, FranceInstitut Pasteur, Biology of Infection Unit, Inserm U1117, Laboratory of Pathogen Discovery, 75015 Paris, FranceVarious methods exist to detect an astrovirus infection. Current methods include electron microscopy (EM), cell culture, immunoassays, polymerase chain reaction (PCR) and various other molecular approaches that can be applied in the context of diagnostic or in surveillance studies. With the advent of metagenomics, novel human astrovirus (HAstV) strains have been found in immunocompromised individuals in association with central nervous system (CNS) infections. This work reviews the past and current methods for astrovirus detection and their uses in both research laboratories and for medical diagnostic purposes.http://www.mdpi.com/1999-4915/9/1/10astrovirusvirologydetection methodsmolecular diagnostics
spellingShingle Philippe Pérot
Marc Lecuit
Marc Eloit
Astrovirus Diagnostics
Viruses
astrovirus
virology
detection methods
molecular diagnostics
title Astrovirus Diagnostics
title_full Astrovirus Diagnostics
title_fullStr Astrovirus Diagnostics
title_full_unstemmed Astrovirus Diagnostics
title_short Astrovirus Diagnostics
title_sort astrovirus diagnostics
topic astrovirus
virology
detection methods
molecular diagnostics
url http://www.mdpi.com/1999-4915/9/1/10
work_keys_str_mv AT philippeperot astrovirusdiagnostics
AT marclecuit astrovirusdiagnostics
AT marceloit astrovirusdiagnostics