Defining basic rules for hardening influenza A virus liquid condensates
In biological systems, liquid and solid-like biomolecular condensates may contain the same molecules but their behaviour, including movement, elasticity, and viscosity, is different on account of distinct physicochemical properties. As such, it is known that phase transitions affect the function of...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-04-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/85182 |
| _version_ | 1797834999499587584 |
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| author | Temitope Akhigbe Etibor Silvia Vale-Costa Sindhuja Sridharan Daniela Brás Isabelle Becher Victor Hugo Mello Filipe Ferreira Marta Alenquer Mikhail M Savitski Maria-João Amorim |
| author_facet | Temitope Akhigbe Etibor Silvia Vale-Costa Sindhuja Sridharan Daniela Brás Isabelle Becher Victor Hugo Mello Filipe Ferreira Marta Alenquer Mikhail M Savitski Maria-João Amorim |
| author_sort | Temitope Akhigbe Etibor |
| collection | DOAJ |
| description | In biological systems, liquid and solid-like biomolecular condensates may contain the same molecules but their behaviour, including movement, elasticity, and viscosity, is different on account of distinct physicochemical properties. As such, it is known that phase transitions affect the function of biological condensates and that material properties can be tuned by several factors including temperature, concentration, and valency. It is, however, unclear if some factors are more efficient than others at regulating their behaviour. Viral infections are good systems to address this question as they form condensates de novo as part of their replication programmes. Here, we used influenza A virus (IAV) liquid cytosolic condensates, AKA viral inclusions, to provide a proof of concept that liquid condensate hardening via changes in the valency of its components is more efficient than altering their concentration or the temperature of the cell. Liquid IAV inclusions may be hardened by targeting vRNP (viral ribonucleoprotein) interactions via the known NP (nucleoprotein) oligomerising molecule, nucleozin, both in vitro and in vivo without affecting host proteome abundance nor solubility. This study is a starting point for understanding how to pharmacologically modulate the material properties of IAV inclusions and may offer opportunities for alternative antiviral strategies. |
| first_indexed | 2024-04-09T14:47:36Z |
| format | Article |
| id | doaj.art-3f7caaf707fb473f862bd3c0a076c3a3 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-09T14:47:36Z |
| publishDate | 2023-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-3f7caaf707fb473f862bd3c0a076c3a32023-05-02T12:03:46ZengeLife Sciences Publications LtdeLife2050-084X2023-04-011210.7554/eLife.85182Defining basic rules for hardening influenza A virus liquid condensatesTemitope Akhigbe Etibor0https://orcid.org/0000-0002-9024-4310Silvia Vale-Costa1Sindhuja Sridharan2Daniela Brás3Isabelle Becher4https://orcid.org/0000-0001-7170-2235Victor Hugo Mello5Filipe Ferreira6Marta Alenquer7https://orcid.org/0000-0003-3062-1222Mikhail M Savitski8https://orcid.org/0000-0003-2011-9247Maria-João Amorim9https://orcid.org/0000-0002-4129-6659Cell Biology of Viral Infection Lab, Instituto Gulbenkian de Ciência, Oeiras, PortugalCell Biology of Viral Infection Lab, Instituto Gulbenkian de Ciência, Oeiras, PortugalEuropean Molecular Biology Laboratory, Heidelberg, GermanyCell Biology of Viral Infection Lab, Instituto Gulbenkian de Ciência, Oeiras, PortugalEuropean Molecular Biology Laboratory, Heidelberg, GermanyCell Biology of Viral Infection Lab, Instituto Gulbenkian de Ciência, Oeiras, PortugalCell Biology of Viral Infection Lab, Instituto Gulbenkian de Ciência, Oeiras, PortugalCell Biology of Viral Infection Lab, Instituto Gulbenkian de Ciência, Oeiras, Portugal; Cell Biology of Viral Infection Lab, Universidade Católica Portuguesa, Católica Medical School, Católica Biomedical Research Centre, Lisbon, PortugalEuropean Molecular Biology Laboratory, Heidelberg, GermanyCell Biology of Viral Infection Lab, Instituto Gulbenkian de Ciência, Oeiras, Portugal; Cell Biology of Viral Infection Lab, Universidade Católica Portuguesa, Católica Medical School, Católica Biomedical Research Centre, Lisbon, PortugalIn biological systems, liquid and solid-like biomolecular condensates may contain the same molecules but their behaviour, including movement, elasticity, and viscosity, is different on account of distinct physicochemical properties. As such, it is known that phase transitions affect the function of biological condensates and that material properties can be tuned by several factors including temperature, concentration, and valency. It is, however, unclear if some factors are more efficient than others at regulating their behaviour. Viral infections are good systems to address this question as they form condensates de novo as part of their replication programmes. Here, we used influenza A virus (IAV) liquid cytosolic condensates, AKA viral inclusions, to provide a proof of concept that liquid condensate hardening via changes in the valency of its components is more efficient than altering their concentration or the temperature of the cell. Liquid IAV inclusions may be hardened by targeting vRNP (viral ribonucleoprotein) interactions via the known NP (nucleoprotein) oligomerising molecule, nucleozin, both in vitro and in vivo without affecting host proteome abundance nor solubility. This study is a starting point for understanding how to pharmacologically modulate the material properties of IAV inclusions and may offer opportunities for alternative antiviral strategies.https://elifesciences.org/articles/85182influenza A virusbiomolecular condensatesviral inclusionsphase transitionsproteome-wide solubility |
| spellingShingle | Temitope Akhigbe Etibor Silvia Vale-Costa Sindhuja Sridharan Daniela Brás Isabelle Becher Victor Hugo Mello Filipe Ferreira Marta Alenquer Mikhail M Savitski Maria-João Amorim Defining basic rules for hardening influenza A virus liquid condensates eLife influenza A virus biomolecular condensates viral inclusions phase transitions proteome-wide solubility |
| title | Defining basic rules for hardening influenza A virus liquid condensates |
| title_full | Defining basic rules for hardening influenza A virus liquid condensates |
| title_fullStr | Defining basic rules for hardening influenza A virus liquid condensates |
| title_full_unstemmed | Defining basic rules for hardening influenza A virus liquid condensates |
| title_short | Defining basic rules for hardening influenza A virus liquid condensates |
| title_sort | defining basic rules for hardening influenza a virus liquid condensates |
| topic | influenza A virus biomolecular condensates viral inclusions phase transitions proteome-wide solubility |
| url | https://elifesciences.org/articles/85182 |
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