Autofluorescence in freshly isolated adult human liver sinusoidal cells
Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2021-12-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | https://www.ejh.it/index.php/ejh/article/view/3337 |
| _version_ | 1818891220242399232 |
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| author | Anett Kristin Larsen Jaione Simón-Santamaría Kjetil Elvevold Bo Göran Ericzon Kim Erlend Mortensen Peter McCourt Bård Smedsrød Karen Kristine Sørensen |
| author_facet | Anett Kristin Larsen Jaione Simón-Santamaría Kjetil Elvevold Bo Göran Ericzon Kim Erlend Mortensen Peter McCourt Bård Smedsrød Karen Kristine Sørensen |
| author_sort | Anett Kristin Larsen |
| collection | DOAJ |
| description | Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment with a lipofuscin autofluorescence quencher reduced autofluorescence, most efficiently in the near UV-area. These results, combined with the knowledge of the very active blood clearance function of LSECs support the notion that lysosomally located autofluorescent material reflected accumulation of lipofuscin in the intact liver. These results illustrate the importance of careful selection of fluorophores, especially when labelling of live cells where the quencher is not compatible. |
| first_indexed | 2024-12-19T17:37:21Z |
| format | Article |
| id | doaj.art-3f7d7211341a433daf08507dbcaf4dd7 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-19T17:37:21Z |
| publishDate | 2021-12-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-3f7d7211341a433daf08507dbcaf4dd72022-12-21T20:12:18ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062021-12-0165410.4081/ejh.2021.3337Autofluorescence in freshly isolated adult human liver sinusoidal cellsAnett Kristin Larsen0Jaione Simón-Santamaría1Kjetil Elvevold2Bo Göran Ericzon3Kim Erlend Mortensen4Peter McCourt5Bård Smedsrød6Karen Kristine Sørensen7Department of Medical Biology, UiT – The Arctic University of Norway, TromsøDepartment of Medical Biology, UiT – The Arctic University of Norway, TromsøDepartment of Medical Biology, UiT – The Arctic University of Norway, TromsøDepartment of Clinical Science, Intervention and Technology (CLINTEC), Karolinska University Hospital, StockholmUniversity Hospital of North Norway, TromsøDepartment of Medical Biology, UiT – The Arctic University of Norway, TromsøDepartment of Medical Biology, UiT – The Arctic University of Norway, TromsøDepartment of Medical Biology, UiT – The Arctic University of Norway, TromsøAutofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment with a lipofuscin autofluorescence quencher reduced autofluorescence, most efficiently in the near UV-area. These results, combined with the knowledge of the very active blood clearance function of LSECs support the notion that lysosomally located autofluorescent material reflected accumulation of lipofuscin in the intact liver. These results illustrate the importance of careful selection of fluorophores, especially when labelling of live cells where the quencher is not compatible.https://www.ejh.it/index.php/ejh/article/view/3337Autofluorescenceendogenous fluorophoresliverendothelial cellslipofuscin |
| spellingShingle | Anett Kristin Larsen Jaione Simón-Santamaría Kjetil Elvevold Bo Göran Ericzon Kim Erlend Mortensen Peter McCourt Bård Smedsrød Karen Kristine Sørensen Autofluorescence in freshly isolated adult human liver sinusoidal cells European Journal of Histochemistry Autofluorescence endogenous fluorophores liver endothelial cells lipofuscin |
| title | Autofluorescence in freshly isolated adult human liver sinusoidal cells |
| title_full | Autofluorescence in freshly isolated adult human liver sinusoidal cells |
| title_fullStr | Autofluorescence in freshly isolated adult human liver sinusoidal cells |
| title_full_unstemmed | Autofluorescence in freshly isolated adult human liver sinusoidal cells |
| title_short | Autofluorescence in freshly isolated adult human liver sinusoidal cells |
| title_sort | autofluorescence in freshly isolated adult human liver sinusoidal cells |
| topic | Autofluorescence endogenous fluorophores liver endothelial cells lipofuscin |
| url | https://www.ejh.it/index.php/ejh/article/view/3337 |
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