Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (<i>Solanum lycopersicum</i>) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses
Iron (Fe) and manganese (Mn) are two essential elements for plants that compete for the same uptake transporters and show conflicting interactions at the regulatory level. In order to understand the differential response to both metal deficiencies in plants, two proteomic techniques (two-dimensional...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/7/3719 |
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| author | Laura Ceballos-Laita Daisuke Takahashi Matsuo Uemura Javier Abadía Ana Flor López-Millán Jorge Rodríguez-Celma |
| author_facet | Laura Ceballos-Laita Daisuke Takahashi Matsuo Uemura Javier Abadía Ana Flor López-Millán Jorge Rodríguez-Celma |
| author_sort | Laura Ceballos-Laita |
| collection | DOAJ |
| description | Iron (Fe) and manganese (Mn) are two essential elements for plants that compete for the same uptake transporters and show conflicting interactions at the regulatory level. In order to understand the differential response to both metal deficiencies in plants, two proteomic techniques (two-dimensional gel electrophoresis and label-free shotgun) were used to study the proteome profiles of roots from tomato plants grown under Fe or Mn deficiency. A total of 119 proteins changing in relative abundance were confidently quantified and identified, including 35 and 91 in the cases of Fe deficiency and Mn deficiency, respectively, with 7 of them changing in both deficiencies. The identified proteins were categorized according to function, and GO-enrichment analysis was performed. Data showed that both deficiencies provoked a common and intense cell wall remodelling. However, the response observed for Fe and Mn deficiencies differed greatly in relation to oxidative stress, coumarin production, protein, nitrogen, and energy metabolism. |
| first_indexed | 2024-03-09T11:46:22Z |
| format | Article |
| id | doaj.art-3f7d9e36546142a7ab841738412556a6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T11:46:22Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3f7d9e36546142a7ab841738412556a62023-11-30T23:21:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01237371910.3390/ijms23073719Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (<i>Solanum lycopersicum</i>) Using Two-Dimensional Electrophoresis and Label-Free Shotgun AnalysesLaura Ceballos-Laita0Daisuke Takahashi1Matsuo Uemura2Javier Abadía3Ana Flor López-Millán4Jorge Rodríguez-Celma5Plant Stress Physiology Group, Plant Nutrition Department, Aula Dei Experimental Station, CSIC, 50059 Zaragoza, SpainUnited Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, JapanUnited Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, JapanPlant Stress Physiology Group, Plant Nutrition Department, Aula Dei Experimental Station, CSIC, 50059 Zaragoza, SpainPlant Stress Physiology Group, Plant Nutrition Department, Aula Dei Experimental Station, CSIC, 50059 Zaragoza, SpainPlant Stress Physiology Group, Plant Nutrition Department, Aula Dei Experimental Station, CSIC, 50059 Zaragoza, SpainIron (Fe) and manganese (Mn) are two essential elements for plants that compete for the same uptake transporters and show conflicting interactions at the regulatory level. In order to understand the differential response to both metal deficiencies in plants, two proteomic techniques (two-dimensional gel electrophoresis and label-free shotgun) were used to study the proteome profiles of roots from tomato plants grown under Fe or Mn deficiency. A total of 119 proteins changing in relative abundance were confidently quantified and identified, including 35 and 91 in the cases of Fe deficiency and Mn deficiency, respectively, with 7 of them changing in both deficiencies. The identified proteins were categorized according to function, and GO-enrichment analysis was performed. Data showed that both deficiencies provoked a common and intense cell wall remodelling. However, the response observed for Fe and Mn deficiencies differed greatly in relation to oxidative stress, coumarin production, protein, nitrogen, and energy metabolism.https://www.mdpi.com/1422-0067/23/7/3719rootshotgun proteomicsMn deficiencyFe deficiencytomato2-DE |
| spellingShingle | Laura Ceballos-Laita Daisuke Takahashi Matsuo Uemura Javier Abadía Ana Flor López-Millán Jorge Rodríguez-Celma Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (<i>Solanum lycopersicum</i>) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses International Journal of Molecular Sciences root shotgun proteomics Mn deficiency Fe deficiency tomato 2-DE |
| title | Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (<i>Solanum lycopersicum</i>) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses |
| title_full | Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (<i>Solanum lycopersicum</i>) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses |
| title_fullStr | Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (<i>Solanum lycopersicum</i>) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses |
| title_full_unstemmed | Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (<i>Solanum lycopersicum</i>) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses |
| title_short | Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (<i>Solanum lycopersicum</i>) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses |
| title_sort | effects of fe and mn deficiencies on the root protein profiles of tomato i solanum lycopersicum i using two dimensional electrophoresis and label free shotgun analyses |
| topic | root shotgun proteomics Mn deficiency Fe deficiency tomato 2-DE |
| url | https://www.mdpi.com/1422-0067/23/7/3719 |
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