Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components
The Smc5/6 complex in Saccharomyces cerevisiae contains six essential non-Smc elements, Nse1-6. With the exception of Nse2 (also known as Mms21), which is an E3 small ubiquitin-like modifier (SUMO) ligase, very little is understood about the role of these components or their contribution to Smc5/6 f...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2016-06-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/5/6/777 |
| _version_ | 1818865585845436416 |
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| author | Denise E. Bustard Lindsay G. Ball Jennifer A. Cobb |
| author_facet | Denise E. Bustard Lindsay G. Ball Jennifer A. Cobb |
| author_sort | Denise E. Bustard |
| collection | DOAJ |
| description | The Smc5/6 complex in Saccharomyces cerevisiae contains six essential non-Smc elements, Nse1-6. With the exception of Nse2 (also known as Mms21), which is an E3 small ubiquitin-like modifier (SUMO) ligase, very little is understood about the role of these components or their contribution to Smc5/6 functionality. Our characterization of Nse5 establishes a previously unidentified relationship between the Smc5/6 complex and factors of the SUMO pathway. Nse5 physically associates with the E2 conjugating enzyme, Ubc9, where contacts are stabilized by non-covalent interactions with SUMO. SUMO also mediates the interactions between Nse5 and the two PIAS family E3 SUMO ligases, Siz1 and Siz2. Cells carrying the nse5-ts1 allele or lacking either SIZ1 or SIZ2 exhibit a reduction in Smc5 sumoylation upon MMS treatment and demonstrate functional redundancy for SUMO mediated events in the presence of DNA damage. Overall, given the extensive connection between Nse5 and components of the SUMO pathway, we speculate that one function of the Smc5/6 complex might be as a scaffold center to enable sumoylation events in budding yeast. |
| first_indexed | 2024-12-19T10:49:54Z |
| format | Article |
| id | doaj.art-3f7eda7b76d3435fb152954a396672f3 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-19T10:49:54Z |
| publishDate | 2016-06-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-3f7eda7b76d3435fb152954a396672f32022-12-21T20:25:03ZengThe Company of BiologistsBiology Open2046-63902016-06-015677778510.1242/bio.018440018440Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway componentsDenise E. Bustard0Lindsay G. Ball1Jennifer A. Cobb2 Department of Biochemistry and Molecular Biology, Robson DNA Science Centre, Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1 Department of Biochemistry and Molecular Biology, Robson DNA Science Centre, Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1 Department of Biochemistry and Molecular Biology, Robson DNA Science Centre, Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1 The Smc5/6 complex in Saccharomyces cerevisiae contains six essential non-Smc elements, Nse1-6. With the exception of Nse2 (also known as Mms21), which is an E3 small ubiquitin-like modifier (SUMO) ligase, very little is understood about the role of these components or their contribution to Smc5/6 functionality. Our characterization of Nse5 establishes a previously unidentified relationship between the Smc5/6 complex and factors of the SUMO pathway. Nse5 physically associates with the E2 conjugating enzyme, Ubc9, where contacts are stabilized by non-covalent interactions with SUMO. SUMO also mediates the interactions between Nse5 and the two PIAS family E3 SUMO ligases, Siz1 and Siz2. Cells carrying the nse5-ts1 allele or lacking either SIZ1 or SIZ2 exhibit a reduction in Smc5 sumoylation upon MMS treatment and demonstrate functional redundancy for SUMO mediated events in the presence of DNA damage. Overall, given the extensive connection between Nse5 and components of the SUMO pathway, we speculate that one function of the Smc5/6 complex might be as a scaffold center to enable sumoylation events in budding yeast.http://bio.biologists.org/content/5/6/777Smc5/6 complexSUMOUbc9PIAS E3 SUMO ligases |
| spellingShingle | Denise E. Bustard Lindsay G. Ball Jennifer A. Cobb Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components Biology Open Smc5/6 complex SUMO Ubc9 PIAS E3 SUMO ligases |
| title | Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components |
| title_full | Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components |
| title_fullStr | Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components |
| title_full_unstemmed | Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components |
| title_short | Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components |
| title_sort | non smc element 5 nse5 of the smc5 6 complex interacts with sumo pathway components |
| topic | Smc5/6 complex SUMO Ubc9 PIAS E3 SUMO ligases |
| url | http://bio.biologists.org/content/5/6/777 |
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